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EP0127304A1
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Process for producing heterologous protein in yeast, expression vehicle therefor, and yeast transformed therewith
Assignee: Genentech Inc
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Filing: 1984-04-24
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Granted: —
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Expires: 2004-04-24
Abstract
Abstract The secretion from yeast of heterologous protein via a preprotein in which the heterologous protein is fused to the signal peptide of a yeast protein. The preprotein is processed by the cells to produce and secrete mature heterologous protein.
Inventors
- 👤 Chung Nan Chang
- 👤 Mark Douglas Matteucci
- 👤 Ronald Arthur Hitzeman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 02:20
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
49 words
A yeast expression vehicle comprising the DNA sequence of a yeast promoter operably linked to at least a substantial portion of a DNA sequence encoding a homologous yeast signal peptide which is connected in translation reading frame to a DNA sequence encoding a protein heterologous to the yeast organism.
Claim 2
Dependent Claim
49 words
A yeast expression vehicle comprising the DNA sequence of a yeast promoter operably linked to at least a substantial portion of a DNA sequence encoding a homologous yeast signal peptide which is connected in translation reading frame to a DNA sequence encoding a protein heterologous to the yeast organism.
Claim 3
Dependent Claim
27 words
An expression vehicle according to Claim 1 wherein the DNA sequence encoding a substantial portion of signal peptide corresponds to that of the signal peptide for invertase.
Claim 4
Dependent Claim
13 words
The expression vehicle of Claim 1 wherein the promoter is the invertase promoter.
Claim 5
Dependent Claim
16 words
The expression vehicle according to Claim 1 wherein the promoter is the yeast 3-phospoglycerate kinase promoter.
Claim 6
Dependent Claim
20 words
The expression vehicle of Claim 2 wherein the promoter is the yeast invertase promoter or the yeast 3-phospoglycerate kinase promoter.
Claim 7
Dependent Claim
19 words
The expression vehicle of Claims 1 to 5 wherein the heterologous protein encoding sequence encodes human leukocyte interferon alpha.
Claim 8
Dependent Claim
13 words
A yeast organism transformed by the expression vehicle of Claims 1 to 6.
Claim 9
Dependent Claim
10 words
The yeast organism of Claim 7 which is Saccharomyces cerevisiae.
Claim 10
Dependent Claim
12 words
A yeast cell culture containing the organism of Claim 7 or 8.
Claim 11
Dependent Claim
37 words
A process for obtaining a protein heterologous to yeast, which comprises transforming a yeast organism with the expression vehicle of Claims 1 to 6; culturing the transformed organism; and recovering the heterologous protein from the culture medium.
Claim 12
Dependent Claim
34 words
A process for secreting a discrete protein heterologous to yeast into the culture medium, which process comprises transforming a yeast organism with expression vehicle of Claims 1 to 6 and culturing the transformed organism.
References Cited (Backward Citations)
EP0088632A2
Cited
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