Patent EP0101228A1 successfully refreshed from Google Patents.
EP0101228A1
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Particle agglutination assay of antigens
Assignee: de Duve Institute
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Filing: 1983-07-29
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Granted: —
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Expires: 2003-07-29
Abstract
Abstract In a particle agglutination assay for an antigen (Ag), there is included in the mixture a limited amount of a substance which binds univalently with a proportion of the Ag present, that Ag which is so bound being unable then to cause agglutination of the particles. In this way, unusually large concentrations of Ag can be assayed in that a proportion of the Ag is bound to the univalent substance and the particle agglutination assay is in effect conducted on the smaller amount of Ag still remaining free in solution.
Inventors
- 👤 Daniel Collet-Cassart
- 👤 Jean-Claude Mareschal
- 👤 Pierre Lucien Masson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 22:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; G01N33/6857; G01N33/53; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/823; Y10S436/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
138 words
A particle agglutination assay for an antigen (Ag) in a liquid sample, which comprises forming a mixture of the liquid sample and finely divided particles bearing a reagent, the reagent being such as to bind with the Ag to cause agglutination of the particles; and measuring the extent of agglutination of the particles and therefrom determining the amount of Ag present; characterised in that there is also included in solution in the reaction mixture a known quantity of a substance which is univalent towards the Ag and is capable of binding therewith so that the Ag so bound is unable to agglutinate the particles, said quantity of substance being insufficient to bind with all the Ag under assay, and wherein the amount of Ag is determined from the extent of agglutination and the said quantity of substance used.
Claim 2
Dependent Claim
138 words
A particle agglutination assay for an antigen (Ag) in a liquid sample, which comprises forming a mixture of the liquid sample and finely divided particles bearing a reagent, the reagent being such as to bind with the Ag to cause agglutination of the particles; and measuring the extent of agglutination of the particles and therefrom determining the amount of Ag present; characterised in that there is also included in solution in the reaction mixture a known quantity of a substance which is univalent towards the Ag and is capable of binding therewith so that the Ag so bound is unable to agglutinate the particles, said quantity of substance being insufficient to bind with all the Ag under assay, and wherein the amount of Ag is determined from the extent of agglutination and the said quantity of substance used.
Claim 3
Dependent Claim
22 words
A method according to claim 1, characterised in that said univalent substance is derived from whole antibody to the Ag under assay.
Claim 4
Dependent Claim
25 words
A method according to claim 2, characterised in that said univalent substance is a fragment of said whole antibody which is univalent to said Ag.
Claim 5
Dependent Claim
22 words
A method according to claim 2, characterised in that said univalent substance is a hybrid antibody which is univalent to said Ag.
Claim 6
Independent Claim
28 words
A method according to any of claims 1 to 4, characterised in that said reagent is whole antibody, or F(ab') 2 fragments of whole antibody, to said Ag.
Claim 7
Independent Claim
32 words
A method according to any of claims 1 to 5, characterised in that the said Ag under assay is C-reactive protein, thyroxin binding globulin, human placental lactogen, prealbumin or pregnancy protein SP1.
Claim 8
Dependent Claim
188 words
In a method of assaying an Ag in a sample by a particle agglutination assay, in which the sample is mixed with a suspension of unagglutinated finely divided particles bearing a reagent which binds with the Ag to cause agglutination of the particles, the amount of Ag being determined by measuring the extent of agglutination and referring to standard results obtained by measuring the extent of agglutination with a range of known amounts of Ag, the improvement whereby amounts of Ag greater than said range can be assayed, which improvement comprises a) establishing new standard results for an assay in which there is also included in the mixture a known quantity of a substance which is univalent towards the Ag and is capable of binding therewith so that Ag so bound is not able to agglutinate the particles, said quantity of substance being insufficient to bind with all the Ag under assay; and b) conducting said assay, using said known quantity of substance, on said sample of Ag and determining from the extent of agglutination and the said new standard results, the amount of Ag in the sample.
Claim 9
Dependent Claim
188 words
In a method of assaying an Ag in a sample by a particle agglutination assay, in which the sample is mixed with a suspension of unagglutinated finely divided particles bearing a reagent which binds with the Ag to cause agglutination of the particles, the amount of Ag being determined by measuring the extent of agglutination and referring to standard results obtained by measuring the extent of agglutination with a range of known amounts of Ag, the improvement whereby amounts of Ag greater than said range can be assayed, which improvement comprises a) establishing new standard results for an assay in which there is also included in the mixture a known quantity of a substance which is univalent towards the Ag and is capable of binding therewith so that Ag so bound is not able to agglutinate the particles, said quantity of substance being insufficient to bind with all the Ag under assay; and b) conducting said assay, using said known quantity of substance, on said sample of Ag and determining from the extent of agglutination and the said new standard results, the amount of Ag in the sample.
References Cited (Backward Citations)
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