EP0083869A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method of immunoassay
Assignee: de Duve Institute
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Filing: 1982-12-23
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Granted: —
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Expires: 2002-12-23
Abstract
Abstract A biological fluid from a first animal species (e.g. human serum) is assayed for an immunogen therein by mixing with the fluid (lg-minus-Fc) fragments of an immunoglobulin from a second animal species (e.g. rabbit), the immunoglobulin being immunospecific to the immunogen or another component of the mixture whereby the immunogen can be determined. Interference can occur from reaction between first animal species antibodies and the said fragments, and in the method of the invention this interference is avoided or overcome, preferably by also including in the mixture different (lg-minus-Fc) immunoglobulin fragments from the second animal species which react with said antibodies but not with said immunogen. These different fragments are preferably aggregated. The method of the invention is particularly applicable to particle agglutination assays.
Inventors
- 👤 Daniel Collet-Cassart
- 👤 Carl-Gustav M. Magnusson
- 👤 Pierre Lucien Masson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 18:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/563
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
106 words
A method of immunoassay of an immunogen in a biological fluid from a first animal species, in which method there is used as a reactant (Ig-minus-Fc) fragments (hereinafter called "specific fragments") of an immune- globulin from a second animal species, in the absence of the whole said immunoglobulin and of the F(c) fragments thereof, the said immunoglobulin being immunologically specific to a component of the assay reaction mixture, characterised in that the method includes the step of avoiding or reducing interference in the assay from reaction between the said "specific fragments" and any first species antibodies reactive therewith which are present in the said biological fluid.
Claim 2
Independent Claim
106 words
A method of immunoassay of an immunogen in a biological fluid from a first animal species, in which method there is used as a reactant (Ig-minus-Fc) fragments (hereinafter called "specific fragments") of an immune- globulin from a second animal species, in the absence of the whole said immunoglobulin and of the F(c) fragments thereof, the said immunoglobulin being immunologically specific to a component of the assay reaction mixture, characterised in that the method includes the step of avoiding or reducing interference in the assay from reaction between the said "specific fragments" and any first species antibodies reactive therewith which are present in the said biological fluid.
Claim 3
Dependent Claim
71 words
A method according to claim 1, characterised in that the said biological fluid is contacted with (Ig-minus-Fc) fragments (hereinafter called "non-specific fragments") of an immunoglobulin from said second animal species, in the absence of the whole said immunoglobulin and of the F(c) fragments thereof, which immunoglobulin does not bind to said component of the assay mixture but does bind to said first species antibodies and thereby removes or reduces said interference.
Claim 4
Dependent Claim
16 words
A method according to claim 2, characterised in that said "non-specific fragments" are F(ab') 2 fragments.
Claim 5
Dependent Claim
16 words
A method according to claim 2 or 3, characterised in that said "non-specific fragments" are aggregated.
Claim 6
Dependent Claim
15 words
A method according to claim 4, characterised in that said "non-specific fragments" are chemically aggregated.
Claim 7
Dependent Claim
26 words
A method according to claim 2 or 3, characterised in that said "non-specific fragments" are coupled to a solid insoluble support or to a soluble substance.
Claim 8
Independent Claim
37 words
A method according to any of claims 2 to 6, wherein the immunoassay is effected by a particle counting technique, characterised in that said "non-specific fragments" are present in solution in the reaction mixture during the assay.
Claim 9
Independent Claim
19 words
A method according to any preceding claim characterised in that the "specific fragments" are F(ab') 2 fragments of IgG.
Claim 10
Independent Claim
123 words
A method of immunoassay of an immunogen in human serum which comprises mixing with the human serum finely divided particles bearing F(ab') 2 fragments of an immunoglobulin from a non-human animal, the mixture being free from the whole said immunoglobulin and from F(c) fragments thereof, the immunoglobulin being immunospecific to a component of the mixture, and assaying the amount of immunogen present by counting the unagglutinated particles, characterised in that there is also included in the mixture the F(ab') 2 fragments of another immunoglobulin from said non-human animal, said another immunoglobulin being non-reactive with said component of the mixture but reactive with human antibodies to animal F(ab') 2 fragments, the said mixture being free from whole said another immunoglobulin and F(c) fragments thereof.
Claim 11
Independent Claim
123 words
A method of immunoassay of an immunogen in human serum which comprises mixing with the human serum finely divided particles bearing F(ab') 2 fragments of an immunoglobulin from a non-human animal, the mixture being free from the whole said immunoglobulin and from F(c) fragments thereof, the immunoglobulin being immunospecific to a component of the mixture, and assaying the amount of immunogen present by counting the unagglutinated particles, characterised in that there is also included in the mixture the F(ab') 2 fragments of another immunoglobulin from said non-human animal, said another immunoglobulin being non-reactive with said component of the mixture but reactive with human antibodies to animal F(ab') 2 fragments, the said mixture being free from whole said another immunoglobulin and F(c) fragments thereof.
Claim 12
Dependent Claim
21 words
A method according to claim 9, characterised in that both said F(ab') 2 fragments are derived from the same animal source.
References Cited (Backward Citations)
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