EP0080108A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Diagnostic reagent and use thereof
Assignee: New York Blood Center
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Filing: 1982-11-09
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Granted: —
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Expires: 2002-11-09
Abstract
Abstract A process for the detection of an antigen or antibody in a specimen which process comprises: a) contacting said specimen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen or antibody in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; b) contacting the washed material of step 'a' with a radioactive material labeled or enzyme labeled antibody or antigen, incubating the so-contacted material and washing the same; and c) effecting radioimmunoassay if said antibody or antigen is radioactive or enzyme labeled immunoassay is said antibody or antigen is enzyme labeled.
Inventors
- 👤 Robert A. Neurath
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 22:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/54306; G01N33/54393
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
114 words
A process for the detection of an antigen in a specimen which process is characterized by: . a) contacting said specimen with a substrate having bound.thereon a mixture of antigens and antibodies to said antiget in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; b) contacting the washed material of step 'a' with a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted material and washing the same; and c) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay is said antibody is enzyme labeled.
Claim 2
Dependent Claim
114 words
A process for the detection of an antigen in a specimen which process is characterized by: . a) contacting said specimen with a substrate having bound.thereon a mixture of antigens and antibodies to said antiget in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; b) contacting the washed material of step 'a' with a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted material and washing the same; and c) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay is said antibody is enzyme labeled.
Claim 3
Dependent Claim
82 words
A process according to claim 1, characte- that. zed in / the washed material of step 'a' is contacted with a hapten conjugated antibody against said antigen of said specimen, the so-contacted material is incubated and washed and thereafter the resultant washed material is contacted with a radioactive material labeled or enzyme labeled anti-hapten antibody, incubated and washed and radioimmunoassay is effected if said anti- hapten antibody is radio labeled or enzyme labeled immunoassay is effected if said.anti-hapten antibody is enzyme labeled.
Claim 4
Dependent Claim
45 words
A process according to claims 1 or 2, characterized in thatsaid substrate having bound thereon a mixture of antigens and antibodies is a protein binding solid support in which the sites free of antigen or antibody are substantially filled with a protein binding site occupier.
Claim 5
Dependent Claim
26 words
A process according to claim 3, characterized in that said protein site occupier is bovine serum albumin, gelatin, a normal human serum or an animal serum.
Claim 6
Dependent Claim
20 words
A process according to claim 3, characterized in that said protein binding solit support is derivatized paper or plastic material.
Claim 7
Dependent Claim
27 words
A process according to claim 3, characterized in that said solid support is a paper derivative; such"as nitrocellulose paper, a diazobenzyloxymethyl paper or a diazo- phenylthioether paper.
Claim 8
Dependent Claim
26 words
A process according to claim 3, characterized in that solid support is or comprises a plastic material, such as polystyrene, a polyvinyl material, or a polyolefin.
Claim 9
Dependent Claim
137 words
µ 8. A process for detecting the presence of an antigen in a specimen which process is characterized by: a) contacting the specimen suspected of containing an antigen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigen being bound to said substrate separately not being in the form of an immune complex, in the presence.of a radioactive material labeled or enzyme labeled antigen, incubating the so-contacted substrate and washing the substrate; b) effecting radioimmunoassay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled; and c) comparing the counts if radioimmunoassay is effected or enzymatic activity if enzyme immunoassay is effected with the counts or enzymatic activity of a control carried out without the presence of said specimen.
Claim 10
Dependent Claim
137 words
µ 8. A process for detecting the presence of an antigen in a specimen which process is characterized by: a) contacting the specimen suspected of containing an antigen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigen being bound to said substrate separately not being in the form of an immune complex, in the presence.of a radioactive material labeled or enzyme labeled antigen, incubating the so-contacted substrate and washing the substrate; b) effecting radioimmunoassay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled; and c) comparing the counts if radioimmunoassay is effected or enzymatic activity if enzyme immunoassay is effected with the counts or enzymatic activity of a control carried out without the presence of said specimen.
Claim 11
Dependent Claim
121 words
A process for the detection of the presence of an antibody in a test specimen which process is characterized by: a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibody and antigen to said antibody in said specimen, said antigens and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; b) contacting the washed material of step 'a' with a radioactive material labeled or enzyme labeled antigen, incubating the same and washing the same; and c) effecting radioimmunoasssay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled.
Claim 12
Dependent Claim
121 words
A process for the detection of the presence of an antibody in a test specimen which process is characterized by: a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibody and antigen to said antibody in said specimen, said antigens and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; b) contacting the washed material of step 'a' with a radioactive material labeled or enzyme labeled antigen, incubating the same and washing the same; and c) effecting radioimmunoasssay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled.
Claim 13
Dependent Claim
77 words
A process according to claim 9, characterized in that following step 'a', the washed material of step 'a' is contacted with a hapten conjugated antigen against said antibody, the so-contacted material is incubated and washed and the resultant washed material is contacted with a radioactive material labeled or enzyme-containing anti-hapten antibody incubated and washed after which radioimmunoassay is effected if said antibody is radio active or enzyme labeled immunoassay is effected if said antibody is enzyme labeled.
Claim 14
Dependent Claim
38 words
A process according to claims 9 or 10, characterized in that said substrate comprises a protein binding solid support whose protein binding sites not occupied by antigen or antibody are substantially filled with a protein binding site occupier.
Claim 15
Dependent Claim
26 words
A process according to claim 11, characterized in that said protein site occupier is bovine serum albumin, gelatin, a normal human serum or an animal serum.
Claim 16
Dependent Claim
27 words
A process.according to claim 11, characterized in that said protein binding solid support is or comprises a material as claimed in anyone of claims 5 to 7.
Claim 17
Dependent Claim
111 words
A process for the detection of the presence of an antibody in a specimen which process comprises: a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibodies and antigens to said antibody in said specimen, said antigen and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted substrate and washing the substrate; b) comparing the counts or enzymatic activity with the counts or enzymatic activity of a control carrier out without the use of said specimen..
Claim 18
Dependent Claim
111 words
A process for the detection of the presence of an antibody in a specimen which process comprises: a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibodies and antigens to said antibody in said specimen, said antigen and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted substrate and washing the substrate; b) comparing the counts or enzymatic activity with the counts or enzymatic activity of a control carrier out without the use of said specimen..
References Cited (Backward Citations)
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