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EP0029104A1
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A composition, test device and method of making it, and method for the determination of an analyte in a liquid
Assignee: Bayer Corp
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Filing: 1980-09-27
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Granted: —
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Expires: 2000-09-27
Abstract
Abstract A composition, test device, method of making a test device and process for determination of an analyte in a liquid sample are disclosed. More particularly, there is provided a composition for the determination of an analyte in a sample which composition comprises an analyte- responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of the analyte, an uncoupler effective to generate an oxidizing substance from the reduced form of said pyridine nucleotide, a peroxidatively active substance, and an indicator which, when oxidized, is not susceptible to reduction by said reduced pyridine nucteotide. The pyridine nucleotide is either nicotine adenine dinucleotide (NAD) or nicotine adenine dinucleotide phosphate (NADP).
Inventors
- 👤 Erma C. Cameron
- 👤 Claude R. Gunter
- 👤 Rodric H. White-Stevens
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 09:44
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/28
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
71 words
A composition for the determination of an analyte in a sample characterized in that the composition comprises: an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of the analyte, an uncoupler effective to generate an oxidizing substance from the reduced form of said pyridine nucleotide, a peroxidatively active substance, and an indicator which, when oxidized, is not susceptible to reduction by said reduced pyridine nucleotide.
Claim 2
Dependent Claim
71 words
A composition for the determination of an analyte in a sample characterized in that the composition comprises: an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of the analyte, an uncoupler effective to generate an oxidizing substance from the reduced form of said pyridine nucleotide, a peroxidatively active substance, and an indicator which, when oxidized, is not susceptible to reduction by said reduced pyridine nucleotide.
Claim 3
Dependent Claim
29 words
A composition according to claim 1 characterized in that said analyteresponsive component further comprises at least one constituent interreactive with said analyte to cause reduction of the pyridine nucleotide.
Claim 4
Dependent Claim
36 words
A composition according to claim 2 characterized in that said at least one interreactive constituent is an analyte-specific enzyme and an enzyme interreactive with the product of the reaction of said analyte-specific enzyme with the analyte.
Claim 5
Dependent Claim
18 words
A composition according to claim 2 characterized in that said interreactive component is a substrate of the analyte.
Claim 6
Dependent Claim
36 words
A composition according to claim 2 characterized in that said at least one interreactive constituent is an analyte-specific substrate and an enzyme interreactive with the product of the reaction of said analyte-specific substrate with the analyte.
Claim 7
Dependent Claim
18 words
A composition according to claim 2 characterized in that said uncoupler comprises an uncoupling hydroxylase and a pseudosubstrate.
Claim 8
Dependent Claim
21 words
A composition according to claims 1 to 6, characterized in that said redox indicator comprises a hydrazone and a single coupler.
Claim 9
Independent Claim
28 words
A device for the determination of an analyte in a sample characterized in that it comprises a carrier incorporated with a composition according to claims 1 to 7.
Claim 10
Dependent Claim
31 words
A process for preparing a device for the determination of an analyte in a sample characterized in that a carrier is incorporated with a composition according to claims 1 to 7.
Claim 11
Independent Claim
34 words
A method for the determination of an analyte in a sample characterized in that said sample is contacted with a composition according to claims 1 to 9 and any resultant color change is observed.
References Cited (Backward Citations)
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