USH201H
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensors from membrane proteins reconstituted in polymerized lipid bilayers
Assignee: United States Department of the Navy
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Filing: 1985-08-23
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Granted: 1987-01-06
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Expires: 2005-08-23
Abstract
Abstract A compact and portable biosensor which can detect the presense of an environmental stimulus is produced by extracting cell membrane proteins which are specific for the stimulus, purifying the proteins, and reincorporating the proteins into synthetic membranes. Electrodes are used to measure the voltage or current changes across the membrane when the proteins in the synthetic membranes react to the specific stimulus. The magnitude of the changes indicate the concentration of the stimulus in the environment.
Inventors
- 👤 Paul Yager
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 08:16
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
111 words
A method for detecting a stimulus such as vapors or dissolved compounds, comprising the steps of: extracting membrane proteins which react to said stimulus from biological cells; reincorporating said membrane proteins into a lipid bilayer, said bilayer being mounted so as to create an external compartment and an internal compartment, said external compartment being accessible to the environment containing said stimulus; contacting said bilayer containing said membrane proteins with the environment containing said stimulus; and measuring the change in voltage or conductivity across said bilayer, said voltage or conductivity change indicating the presence of said stimulus, the magnitude of said voltage or conductivity change indicating the amount of said stimulus present.
Claim 2
Independent Claim
111 words
A method for detecting a stimulus such as vapors or dissolved compounds, comprising the steps of: extracting membrane proteins which react to said stimulus from biological cells; reincorporating said membrane proteins into a lipid bilayer, said bilayer being mounted so as to create an external compartment and an internal compartment, said external compartment being accessible to the environment containing said stimulus; contacting said bilayer containing said membrane proteins with the environment containing said stimulus; and measuring the change in voltage or conductivity across said bilayer, said voltage or conductivity change indicating the presence of said stimulus, the magnitude of said voltage or conductivity change indicating the amount of said stimulus present.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein said lipid bilayer in said reincorporating step consists esentially of polymerizable lipids.
Claim 4
Dependent Claim
21 words
The method of claim 2 wherein said polymerizable lipids are selected from the group consisting of di-(10,12-tricosadiynoyl) phosphatidylcholine and di-(10,12-uncosadiynoyl) phosphatidylcholine.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein said membrane proteins in said reincorporating step are acetylcholine receptor proteins.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein said voltage or conductivity in said measuring step is measured using an electrode pair.
Claim 7
Dependent Claim
15 words
The method of claim 5 wherein said electrode pair is a silver-silver chloride electrode pair.
Claim 8
Dependent Claim
130 words
A biosensing device suitable for detecting a stimulus such as vapors or dissolved compounds, comprising: means for supporting a lipid bilayer; a lipid bilayer mounted on said supporting means, said suporting means and lipid bilayer creating an external compartment and an internal compartment, said external compartment being accessible to the environment containing said stimulus; membrane proteins which react specifically to said stimulus, said membrane proteins having been extracted from biological cell membranes, purified, and reincorporated into said lipid bilayer; means for facilitating the measurement of the current or voltage change across said lipid bilayer; and a shield that surrounds said supporting means and mounted bilayer for protecting said microelectrode and bilayer from external disruption, said shield having a semipermeable section in one end to allow access to the external environment.
Claim 9
Dependent Claim
130 words
A biosensing device suitable for detecting a stimulus such as vapors or dissolved compounds, comprising: means for supporting a lipid bilayer; a lipid bilayer mounted on said supporting means, said suporting means and lipid bilayer creating an external compartment and an internal compartment, said external compartment being accessible to the environment containing said stimulus; membrane proteins which react specifically to said stimulus, said membrane proteins having been extracted from biological cell membranes, purified, and reincorporated into said lipid bilayer; means for facilitating the measurement of the current or voltage change across said lipid bilayer; and a shield that surrounds said supporting means and mounted bilayer for protecting said microelectrode and bilayer from external disruption, said shield having a semipermeable section in one end to allow access to the external environment.
Claim 10
Dependent Claim
14 words
The device of claim 7 wherein said lipid bilayer consists esentially of polymerizable lipids.
Claim 11
Dependent Claim
21 words
The device of claim 8 wherein said polymerizable lipids are selected from the group consisting of di-(10,12-tricosadiynoyl) phosphatidylcholine and di-(10,12-uncosadiynoyl) phosphatidylcholine.
Claim 12
Dependent Claim
17 words
The device of claim 7 wherein said means for supporting a lipid bilayer is a glass microelectrode.
Claim 13
Dependent Claim
26 words
The device of claim 7 wherein said means for facilitating the measurement of the current or voltage change across said lipid bilayer is an electrode pair.
Claim 14
Dependent Claim
15 words
The device of claim 11 wherein said electrode pair is a silver-silver chloride electrode pair.
Claim 15
Dependent Claim
13 words
The device of claim 7 wherein said membrane proteins are acetylcholine receptor proteins.
Claim 16
Dependent Claim
160 words
A biosensing device suitable for detecting a stimulus such as vapors or dissolved compounds, comprising: a glass microelectrode having an opening at one end; a polymerizable lipid bilayer for containing reincorporated membrane proteins mounted over said opening at one end of said microelectrode, said polymerizable lipid bilayer creating an internal compartment and an external compartment; acetylcholine receptor membrane proteins which have been extracted from biological cell membranes, purified, and reincorporated into said polymerizable lipid bilayer; a silver-silver chloride electrode pair, the first electrode of said pair being mounted in the internal compartment of said glass microelectrode, the second electrode of said electrode pair being mounted in the external compartment to said microelectrode tip, said external compartment being accessible to the environment containing said stimulus; and a shield surrounding said microelectrode tip and mounted bilayer for protecting said microelectrode and polymerized bilayer from external disruption, said shield having a semipermable section in one end to allow access to the external environment.
Claim 17
Dependent Claim
160 words
A biosensing device suitable for detecting a stimulus such as vapors or dissolved compounds, comprising: a glass microelectrode having an opening at one end; a polymerizable lipid bilayer for containing reincorporated membrane proteins mounted over said opening at one end of said microelectrode, said polymerizable lipid bilayer creating an internal compartment and an external compartment; acetylcholine receptor membrane proteins which have been extracted from biological cell membranes, purified, and reincorporated into said polymerizable lipid bilayer; a silver-silver chloride electrode pair, the first electrode of said pair being mounted in the internal compartment of said glass microelectrode, the second electrode of said electrode pair being mounted in the external compartment to said microelectrode tip, said external compartment being accessible to the environment containing said stimulus; and a shield surrounding said microelectrode tip and mounted bilayer for protecting said microelectrode and polymerized bilayer from external disruption, said shield having a semipermable section in one end to allow access to the external environment.
Claim 18
Dependent Claim
21 words
The method of claim 14 wherein said polymerizable lipids are selected from the group consisting of di-(10,12-tricosadiynoyl) phosphatidylcholine and di-(10,12-uncosadiynoyl) phosphatidylcholine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-01-05 | 1990-07-07 | 1991-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-05 | 1994-07-07 | 1995-01-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-01-05 | 1998-07-07 | 1999-01-06 | $7700.00 | Window Closed / Lapsed |