Patent US9724373B2 successfully refreshed from Google Patents.
US9724373B2
Active / In Force
US
⏱️ 8.1 years remaining
FOS-based prebiotic and bacterial-based probiotic
Assignee: MicroBios Inc
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Filing: 2014-12-18
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Granted: 2017-08-08
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Expires: 2034-12-18
Abstract
Abstract Composition including a pathogen inhibiting bacterial probiotic and a fructooligosaccharide (FOS) prebiotic particularly suited as a feed additive administered to juvenile animals such as calves that promotes animal health and growth, reduces scours and mortality, and can act as an antibiotic substitute. The additive inhibits pathogen growth resulting in lower pathogen load within the animal and pathogen sheading. The prebiotic-probiotic additive includes bacteria that inhibit E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, as well as the Big-Six Escherichia coli strains. The probiotic is constituted of various combinations of four pathogen-inhibiting bacteria: Lactobacillus animalis; Lactobacillus animalis; Enterococcus faecium ; and Pediococcus acidilactici.
Inventors
- 👤 Joseph F. Flint
- 👤 Matthew Ryan Garner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 17:15
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K35/741; A61K35/744; A61K35/747; A23; A23K; A23K10/00; A23K10/10; A23K10/16; A23K10/18; A23K50/00; A23K50/60; A61K2300/00
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
105 words
A prebiotic-probiotic feed additive comprising fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 2
Dependent Claim
105 words
A prebiotic-probiotic feed additive comprising fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 3
Dependent Claim
48 words
The feed additive recited in claim 1 wherein the mixture has a Salmonella typhimurium growth inhibition rate of at least 97.4% when mixed with an equal number of viable Salmonella typhimurium bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 4
Dependent Claim
48 words
The feed additive recited in claim 2 wherein the mixture has a Salmonella enteriditis growth inhibition rate of at least 96.0% when mixed with an equal number of viable Salmonella enteriditis bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 5
Dependent Claim
48 words
The feed additive recited in claim 1 wherein the mixture has a Salmonella enteriditis growth inhibition rate of at least 96.0% when mixed with an equal number of viable Salmonella enteriditis bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 6
Dependent Claim
25 words
The feed additive recited in claim 4 wherein said equal number of viable bacteria is 5×10 5 to 1×10 12 cfu/gram of packaged dry matter.
Claim 7
Dependent Claim
48 words
The feed additive recited in claim 1 wherein the mixture is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains comprising E. coli O 121:H19; E. coli O 45:H2; E. coli O 103:H11; E. coli O 145:NM, E. coli O 26:H11; and E. coli O 111.
Claim 8
Dependent Claim
38 words
The feed additive recited in claim 1 further comprising at least one prebiotic chosen from the group comprising: inulins, isomalto-oligosaccharides, gentio-oligosaccharides, lactilol, lactosucrose, lactulose, xylosucrose, glycosyl sucrose, pyrodextrins, soybean oligosaccharides, galacto-oligosaccharides (GOS), transgalactose-oligosaccharides, xylo-oligosaccharides (XOS), and Malto-oligosaccharides (MOS).
Claim 9
Dependent Claim
44 words
The feed additive recited in claim 1 further comprising at least one prebiotic chosen from the group comprising: mannan such as guar gum, locust bean gum, konjacor xanthan gum, pentosan, beta-glucan, arabinan, galactan, palantinose-oligosaccharides, gluco-oligosaccharides, cyclo-inulo-oligosaccharides, chito-oligosaccharides, agaro-oligosaccharides, neo-oligosaccharides, garo-oligosaccharides, pectins and pectic polysaccharides.
Claim 10
Dependent Claim
18 words
The feed additive recited in claim 1 wherein each strain in the mixture is a pathogen growth inhibitor.
Claim 11
Dependent Claim
20 words
The feed additive recited in claim 1 wherein each strain in the mixture is an E. coli O157:H7 growth inhibitor.
Claim 12
Dependent Claim
50 words
The feed additive recited in claim 1 wherein each strain in the mixture is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains including E. coli O 121:H19; E. coli O 45:H2; E. coli O 103:H11; E. coli O145:NM, E. coli O 26:H11; and E. coli O 111.
Claim 13
Dependent Claim
18 words
The feed additive recited in claim 1 wherein each strain in the mixture is a Salmonella growth inhibitor.
Claim 14
Dependent Claim
19 words
The feed additive recited in claim 1 wherein each strain in the mixture is a Salmonella typhimurium growth inhibitor.
Claim 15
Dependent Claim
19 words
The feed additive recited in claim 1 wherein each strain in the mixture is a Salmonella enteriditis growth inhibitor.
Claim 16
Independent Claim
127 words
A method of administering a prebiotic-probiotic feed additive to an animal and inhibiting pathogen growth within the animal's digestive tract, the method comprising: feeding to an animal fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 17
Independent Claim
127 words
A method of administering a prebiotic-probiotic feed additive to an animal and inhibiting pathogen growth within the animal's digestive tract, the method comprising: feeding to an animal fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 18
Dependent Claim
17 words
The method recited in claim 15 wherein each strain in the mixture is a pathogen growth inhibitor.
Claim 19
Dependent Claim
19 words
The method recited in claim 15 wherein each strain in the mixture is an E. coli O157:H7 growth inhibitor.
Claim 20
Dependent Claim
50 words
The method recited in claim 15 wherein each strain in the mixture is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains including E. coli O 121:H19; E. coli O 45:H2; E. coli O 103:H11; E. coli O 145:NM, E. coli O 26:H11; and E. coli O 111.
Claim 21
Dependent Claim
17 words
The method recited in claim 15 wherein each strain in the mixture is a Salmonella growth inhibitor.
Claim 22
Dependent Claim
113 words
A prebiotic-probiotic food for animals including animal feed mixed with a composition comprising fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 23
Dependent Claim
113 words
A prebiotic-probiotic food for animals including animal feed mixed with a composition comprising fructooligosaccharide (FOS) and a mixture having an equal number of viable bacteria of each of the following four bacteria: Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and wherein the mixture has an E. coli O157:H7 growth inhibition rate of at least 93.1% when mixed with an equal number of viable E. coli O157:H7 bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 24
Dependent Claim
50 words
The prebiotic-probiotic food for animals recited in claim 20 wherein the mixture has a Salmonella typhimurium growth inhibition rate of at least 97.4% when mixed with an equal number of viable Salmonella typhimurium bacteria relative to the total number of viable bacteria comprised by the four bacteria of the mixture.
Claim 25
Dependent Claim
20 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is a pathogen growth inhibitor.
Claim 26
Dependent Claim
22 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is an E. coli O157:H7 growth inhibitor.
Claim 27
Dependent Claim
53 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains including E. coli O 121:H19; E. coli O 45:H2; E. coli O 103:H11; E. coli O 145:NM, E. coli O 26:H11; and E. coli O 111.
Claim 28
Dependent Claim
20 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is a Salmonella growth inhibitor.
Claim 29
Dependent Claim
21 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is a Salmonella typhimurium growth inhibitor.
Claim 30
Dependent Claim
21 words
The prebiotic-probiotic food for animals recited in claim 20 wherein each strain in the mixture is a Salmonella enteriditis growth inhibitor.
Claim 31
Dependent Claim
50 words
The prebiotic-probiotic food for animals recited in claim 20 further comprising at least one prebiotic chosen from the group comprising: inulins, isomalto-oligosaccharides, gentio-oligosaccharides, lactilol, lactosucrose, lactulose, xylosucrose, glycosyl sucrose, pyrodextrins, soybean oligosaccharides, galacto-oligosaccharides (GOS), transgalactose-oligosaccharides, xylo-oligosaccharides (XOS), Malto-oligosaccharides (MOS), palantinose-oligosaccharides, gluco-oligosaccharides, cyclo-inulo-oligosaccharides, chito-oligosaccharides, agaro-oligosaccharides, neo-oligosaccharides, garo-oligosaccharides, pectins and pectic polysaccharides.
Claim 32
Dependent Claim
24 words
The method of claim 15 , wherein said equal number of viable bacteria is from 5×10 5 to 1×10 12 cfu/gram of dry matter.
Claim 33
Dependent Claim
25 words
The prebiotic-probiotic food of claim 20 , wherein said equal number of viable bacteria is from 5×10 5 to 1×10 12 cfu/gram of dry matter.
References Cited (Backward Citations)
US20070286844A1
Cited
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