US6881825B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Identication of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and virues
Assignee: University of Pittsburgh
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Filing: 2000-08-31
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Granted: 2005-04-19
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Expires: 2020-08-31
Abstract
Abstract The present invention relates to internalizing peptides which facilitate the uptake and transport of cargo into the cytoplasm and nuclei of cells as well as methods for the identification of the peptides, and methods of use for the peptides. The internalizing peptides of the present invention are selected for their ability to efficiently internalize cargo into a wide variety of cell types both in vivo and in vitro.
Inventors
- 👤 Paul D. Robbins
- 👤 Zhibao Mi
- 👤 Raymond Frizzell
- 👤 Joseph C. Glorioso
- 👤 Andrea Gambotto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:04
CPC Classifications
C; C40; C40B; C40B40/00; C40B40/02; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/62; C07; C07K; C07K7/00; C07K7/04; C07K7/06; C07K7/08; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1037; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6842; A61K38/00; A61K39/00; A61K48/00; C07K2319/00; C07K2319/01; C07K2319/10; G01N2500/00; G01N2500/10
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
38 words
A peptide having a amino acid sequence selected from the group consisting of PIRRRKKLRRLK (SEQ ID NO:4); RRQRRTSKLMKR (SEQ ID NO:5); SRRKRQRSNMRI (SEQ ID NO:9); SFHQFARATLAS (SEQ ID NO:33); DPATNPGPHFPR (SEQ ID NO:58); and TLPSPLALLTVH (SEQ ID NO:59).
Claim 2
Dependent Claim
38 words
A peptide having a amino acid sequence selected from the group consisting of PIRRRKKLRRLK (SEQ ID NO:4); RRQRRTSKLMKR (SEQ ID NO:5); SRRKRQRSNMRI (SEQ ID NO:9); SFHQFARATLAS (SEQ ID NO:33); DPATNPGPHFPR (SEQ ID NO:58); and TLPSPLALLTVH (SEQ ID NO:59).
Claim 3
Dependent Claim
16 words
The peptide of claim 1 wherein said peptide facilitates cellular internalization of a cargo linked thereto.
Claim 4
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is PIRRRKKLRRLK (SEQ ID NO:4).
Claim 5
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is RRQRRTSKLMKR (SEQ ID NO:5).
Claim 6
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is SRRKRQRSNMRI (SEQ ID NO:9).
Claim 7
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is SFHQFARATLAS (SEQ ID NO:33).
Claim 8
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is DPATNPGPHFPR (SEQ ID NO:58).
Claim 9
Dependent Claim
13 words
The peptide of claim 2 wherein the peptide is TLPSPLALLTVH (SEQ ID NO:59).
Claim 10
Dependent Claim
16 words
The peptide of claim 1 wherein the peptide provides for nuclear translocation in a target cell.
Claim 11
Independent Claim
55 words
A method for inducing synovial cell death comprising administering a peptide-cargo complex to said synovial cell wherein the peptide has an amino acid sequence selected from the group consisting of PIRRRKKLRRLK (SEQ ID NO:4), RRQRRTSKLMKR (SEQ ID NO:5), SRRKRQRSNMRI (SEQ ID NO:9), SFHQFARATLAS (SEQ ID NO:33), DPATNPGPHFPR (SEQ ID NO:58) and TLPSPLALLTVH (SEQ ID NO:59).
Claim 12
Independent Claim
55 words
A method for inducing synovial cell death comprising administering a peptide-cargo complex to said synovial cell wherein the peptide has an amino acid sequence selected from the group consisting of PIRRRKKLRRLK (SEQ ID NO:4), RRQRRTSKLMKR (SEQ ID NO:5), SRRKRQRSNMRI (SEQ ID NO:9), SFHQFARATLAS (SEQ ID NO:33), DPATNPGPHFPR (SEQ ID NO:58) and TLPSPLALLTVH (SEQ ID NO:59).
Claim 13
Dependent Claim
12 words
The method of claim 10 wherein the cargo is an apoptotic protein.
Claim 14
Dependent Claim
25 words
The method of claim 10 where the apoptotic protein is selected from the group consisting of p53, caspase-3, HSV thymidine kinase and an antimicrobial peptide.
Claim 15
Dependent Claim
29 words
The method of claim 12 wherein the antimicrobial peptide has an amino acid sequence selected from the group consisting of KLAKLAK (SEQ ID NO:22) and KLAKLAKKLAKLAK (SEQ ID NO:23).
Claim 16
Dependent Claim
18 words
The method of claim 10 wherein the peptide-cargo complex has an amino acid sequence RRQRRTSKLMKRGGKLAKLAKKLAKLAK (SEQ ID NO:24).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2008-04-18 | 2008-10-18 | 2009-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2012-04-18 | 2012-10-18 | 2013-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2016-04-18 | 2016-10-18 | 2017-04-19 | $7700.00 | Window Closed / Lapsed |