US6875442B2
Expired / Lapsed
US
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Process for growing tissue in a biocompatible macroporous polymer scaffold and products therefrom
Assignee: Bonetec Corp
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Filing: 2002-08-13
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Granted: 2005-04-05
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Expires: 2022-08-13
Abstract
Abstract A polymer scaffold is provided comprising an extensively interconnected macroporous network. The polymer scaffold embodies macropores having a diameter in a range of 0.5-3.5 mm, and preferably in a range of about 1.0-2.0 mm. The polymer scaffold is prepared using a novel process which advantageously combines the techniques of particulate leaching and phase inversion to render a process that provides amplified means by which to control the morphology of the resulting polymer scaffold. The polymer scaffold has utility in the area of tissue engineering, particularly as a scaffold for both in vitro and in vivo cell growth.
Inventors
- 👤 Chantal E. Holy
- 👤 Molly S. Shoichet
- 👤 John E. Davies
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:23
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L27/00; A61L27/14; A61L27/18; A61L27/36; A61L27/38; A61L27/3804; A61L27/3821; A61L27/3839; A61L27/3843; A61L27/3847; A61L27/50; A61L27/56; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/096; C12N2533/00; C12N2533/30; C12N2533/40
Claims Analysis
Total Claims: 48
Claim 1
Independent Claim
109 words
A tissue implant prepared by seeding a biocompatible macroporous polymer scaffold with tissue cells and culturing said tissue cells, said biocompatible macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, and said macroporous polymer scaffold having a porosity of at least 50%.
Claim 2
Dependent Claim
109 words
A tissue implant prepared by seeding a biocompatible macroporous polymer scaffold with tissue cells and culturing said tissue cells, said biocompatible macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, and said macroporous polymer scaffold having a porosity of at least 50%.
Claim 3
Dependent Claim
53 words
A tissue implant as defined in claim 1 wherein said macroporous passageways connecting macropores of said macroporous polymer scaffold have a mean diameter in a range from about 200 μm to about 2 mm, and wherein said microporous passageways have a mean diameter greater than 50 μm and less than about 200 μm.
Claim 4
Dependent Claim
21 words
A tissue implant as defined in claim 2 wherein said microporous polymer struts have a thickness of less than 0.4 mm.
Claim 5
Dependent Claim
15 words
A tissue implant as defined in claim 1 wherein said macroporous polymer scaffold is biodegradable.
Claim 6
Dependent Claim
13 words
A tissue implant as defined in claim 4 wherein the polymer is poly(lactide-co-glycolide).
Claim 7
Dependent Claim
22 words
A tissue implant as defined in claim 5 wherein the polymer comprises poly(lactide-co-glycolide) in a ratio of 75% lactide and 25% glycolide.
Claim 8
Dependent Claim
36 words
A tissue implant as defined in claim 1 wherein said microporous polymer struts have a thickness of less than 0.4 mm, and wherein said polymer scaffold is biodegradable and has a porosity of at least 85%.
Claim 9
Dependent Claim
20 words
A tissue implant as defined in claim 2 wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 10
Dependent Claim
19 words
A tissue implant as defined in claim 1 , wherein said macroporous polymer scaffold treated to modify its surface.
Claim 11
Dependent Claim
43 words
A tissue implant as defined in claim 9 wherein the surface of the macroporous polymer scaffold is modified using a treatment selected from the group consisting of acid treatment, base treatment, ammonia plasma modification, collagen deposition and calcium phosphate deposition, platelet-rich plasma deposition/exposure.
Claim 12
Dependent Claim
15 words
A tissue implant as defined in claim 1 wherein said tissue cells are osteogenic cells.
Claim 13
Dependent Claim
15 words
A tissue implant as defined in claim 11 wherein said tissue cells elaborate bone matrix.
Claim 14
Dependent Claim
16 words
A tissue implant as defined in claim 12 wherein said tissue cells are of human origin.
Claim 15
Dependent Claim
35 words
A tissue implant as defined in claim 13 wherein said tissue cells are selected from the group consisting of paradontal tissue cells, cartilage tissue cells, dental tissue cells, liver tissue cells and breast tissue cells.
Claim 16
Dependent Claim
17 words
A tissue implant as defined in claim 1 wherein said cells are of mesenchymal or hematopoietic origin.
Claim 17
Dependent Claim
20 words
A tissue implant as defined in claim 1 wherein said cells are maintained for in vitro and in vivo applications.
Claim 18
Dependent Claim
21 words
A tissue implant as defined in claim 4 , wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 19
Dependent Claim
143 words
A tissue implant prepared by seeding a biocompatible macroporous polymer scaffold with tissue cells and culturing said tissue cells, said biocompatible macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, said macroporous passageways connecting macropores having a mean diameter in a range from about 200 μm to about 2 mm, and wherein said microporous passageways have a mean diameter less than about 200 μm, and said macroporous polymer scaffold having a porosity of at least 50%.
Claim 20
Dependent Claim
143 words
A tissue implant prepared by seeding a biocompatible macroporous polymer scaffold with tissue cells and culturing said tissue cells, said biocompatible macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, said macroporous passageways connecting macropores having a mean diameter in a range from about 200 μm to about 2 mm, and wherein said microporous passageways have a mean diameter less than about 200 μm, and said macroporous polymer scaffold having a porosity of at least 50%.
Claim 21
Dependent Claim
22 words
A tissue implant as defined in claim 18 wherein said microporous polymer struts have a thickness of less than about 0.4 mm.
Claim 22
Dependent Claim
16 words
A tissue implant as defined in claim 18 , wherein said macroporous polymer scaffold is biodegradable.
Claim 23
Dependent Claim
13 words
A tissue implant as defined in claim 20 wherein the polymer is poly(lactide-co-glycolide).
Claim 24
Dependent Claim
22 words
A tissue implant as defined in claim 21 wherein the polymer comprises poly(lactide-co-glycolide) in a ratio of 75% lactide and 25% glycolide.
Claim 25
Dependent Claim
21 words
A tissue implant as defined in claim 21 , wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 26
Dependent Claim
37 words
A tissue implant as defined in claim 18 wherein said microporous polymer struts have a thickness of less than about 0.4 mm, and wherein said polymer scaffold is biodegradable, and has a porosity of at least 85%.
Claim 27
Dependent Claim
20 words
A tissue implant as defined in claim 18 wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 28
Dependent Claim
20 words
A tissue implant as defined in claim 18 , wherein said macroporous polymer scaffold is treated to modify its surface.
Claim 29
Dependent Claim
43 words
A tissue implant as defined in claim 26 wherein the surface of the macroporous polymer scaffold is modified using a treatment selected from the group consisting of acid treatment, base treatment, ammonia plasma modification, collagen deposition and calcium phosphate deposition, platelet-rich plasma deposition/exposure.
Claim 30
Dependent Claim
15 words
A tissue implant as defined in claim 18 wherein said tissue cells are osteogenic cells.
Claim 31
Dependent Claim
15 words
A tissue implant as defined in claim 28 wherein said tissue cells elaborate bone matrix.
Claim 32
Dependent Claim
16 words
A tissue implant as defined in claim 29 wherein said tissue cells are of human origin.
Claim 33
Dependent Claim
35 words
A tissue implant as defined in claim 30 wherein said tissue cells are selected from the group consisting of paradontal tissue cells, cartilage tissue cells, dental tissue cells, liver tissue cells and breast tissue cells.
Claim 34
Dependent Claim
17 words
A tissue implant as defined in claim 18 wherein said cells are of mesenchymal or hematopoietic origin.
Claim 35
Dependent Claim
20 words
A tissue implant as defined in claim 18 wherein said cells are maintained for in vitro and in vivo applications.
Claim 36
Dependent Claim
21 words
A tissue implant as defined in claim 20 , wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 37
Dependent Claim
124 words
A process for growing tissue, with pervasive distribution, in a macroporous polymer scaffold including macropores to a depth of at least 2.5 times an average macropore size in the scaffold, comprising the steps of: providing a macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, and said macroporous polymer scaffold having a porosity of at least 50%;
Claim 38
Dependent Claim
124 words
A process for growing tissue, with pervasive distribution, in a macroporous polymer scaffold including macropores to a depth of at least 2.5 times an average macropore size in the scaffold, comprising the steps of: providing a macroporous polymer scaffold comprising porous walls that are essentially non-membranous, said porous walls consisting of microporous polymer struts defining macropores which are interconnected by macroporous passageways, said microporous polymer struts containing microporous passageways extending through said microporous polymer struts so that macropores on either side of a given microporous polymer strut are in communication through said given microporous polymer strut, said macropores having a mean diameter in a range from about 0.5 to about 3.5 mm, and said macroporous polymer scaffold having a porosity of at least 50%;
Claim 39
Dependent Claim
49 words
A process for growing tissue as defined in claim 35 wherein said macroporous passageways connecting macropores have a mean diameter in a range from about 200 μm to about 2 mm, and wherein said microporous passageways have a mean diameter greater than 50 μm and less than 200 μm.
Claim 40
Dependent Claim
22 words
A process for growing tissue as defined in claim 36 wherein said macroporous polymer scaffold has a porosity of at least 85%.
Claim 41
Dependent Claim
23 words
A process for growing tissue as defined in claim 35 , additionally comprising the step of modifying the surface of the polymer scaffold.
Claim 42
Dependent Claim
41 words
A process as defined in claim 38 wherein the surface of the polymer scaffold is modified using a treatment selected from the group consisting of acid treatment, base treatment, ammonia plasma modification, collagen deposition and calcium phosphate deposition, platelet-rich plasma deposition/exposure.
Claim 43
Dependent Claim
14 words
A process as defined in claim 35 wherein said tissue cells are osteogenic cells.
Claim 44
Dependent Claim
14 words
A process as defined in claim 40 wherein said tissue cells elaborate bone matrix.
Claim 45
Dependent Claim
15 words
A process as defined in claim 41 wherein said tissue cells are of human origin.
Claim 46
Dependent Claim
34 words
A process as defined in claim 42 whereln said tissue cells are selected from the group consisting of paradontal tissue cells, cartilage tissue cells, dental tissue cells, liver tissue cells and breast tissue cells.
Claim 47
Dependent Claim
15 words
A process as defined in claim 35 wherein cells are of mesenchymal or hematopoietic origin.
Claim 48
Dependent Claim
19 words
A process as defined in claim 35 wherein said cells are maintained for in vitro and in vivo applications.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2008-04-04 | 2008-10-04 | 2009-04-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2012-04-04 | 2012-10-04 | 2013-04-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2016-04-04 | 2016-10-04 | 2017-04-05 | $7700.00 | Window Closed / Lapsed |