Patent US6589727B1 successfully refreshed from Google Patents.
US6589727B1
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US
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Measurement and use of molecular interactions
Assignee: Alere Switzerland GmbH
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Filing: 2000-04-25
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Granted: 2003-07-08
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Expires: 2020-04-25
Abstract
Abstract A method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of:(i) contacting the binding partners, one of which is immobilised on a surface;(ii) oscillating the surface at increasing amplitude; and(iii) detecting a dissociation event.An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and a device (14, 15) for detecting a dissociation event.
Inventors
- 👤 David Klenerman
- 👤 Victor Petrovich Ostanin
- 👤 Fedor Nikolaievich Dultsev
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 23:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
98 words
A method for determining the affinity between first and second binding partners, which comprises the steps of: (i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other; (ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and (iii) detecting the dissociation of the first and second binding partners, wherein the affinity of the first and second binding partners is determined from the dissociation event.
Claim 2
Independent Claim
98 words
A method for determining the affinity between first and second binding partners, which comprises the steps of: (i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other; (ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and (iii) detecting the dissociation of the first and second binding partners, wherein the affinity of the first and second binding partners is determined from the dissociation event.
Claim 3
Dependent Claim
21 words
The method according to claim 1 , wherein the second binding partner is a particle or is immobilised on a particle.
Claim 4
Dependent Claim
24 words
The method according to claim 1 , wherein the first or second binding partner is a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 5
Dependent Claim
22 words
The method according to claim 1 , wherein the first or second binding partner is a cell, bacterium, virus, prion, or phage.
Claim 6
Dependent Claim
19 words
The method according to claim 1 , wherein different binding partners are immobilised at different positions on the surface.
Claim 7
Dependent Claim
16 words
The method according to claim 1 , wherein the dissociation event is detected by acoustic emission.
Claim 8
Dependent Claim
17 words
The method according to claim 1 , wherein the surface is a piezoelectric transducer or acoustic transducer.
Claim 9
Dependent Claim
20 words
The method according to claim 7 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 10
Dependent Claim
14 words
The method according to claim 1 , which is carried out in a liquid.
Claim 11
Dependent Claim
25 words
The method according to claim 1 , wherein the first and second binding partners are, independently, a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 12
Dependent Claim
23 words
The method according to claim 1 , wherein the first and second binding partners are, independently, a cell, bacterium, virus, prion, or phage.
Claim 13
Dependent Claim
14 words
The method according to claim 1 , wherein the increasing amplitude is monotonously increasing.
Claim 14
Dependent Claim
22 words
The method according to claim 1 , which additionally comprises the step: (iv) measuring the voltage at which the disassociation event occurs.
Claim 15
Independent Claim
72 words
A method for separating a target analyte from a composition, which comprises the steps of: (i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and (ii) oscillating the surface such that the amplitude increases to a value wherein the analyte, or other components of the composition, are selectively removed or dissociated from the surface, thereby separating the target analyte from the composition.
Claim 16
Independent Claim
72 words
A method for separating a target analyte from a composition, which comprises the steps of: (i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and (ii) oscillating the surface such that the amplitude increases to a value wherein the analyte, or other components of the composition, are selectively removed or dissociated from the surface, thereby separating the target analyte from the composition.
Claim 17
Dependent Claim
25 words
The method according to claim 14 , wherein the binding partner or the target analyte is a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 18
Dependent Claim
23 words
The method according to claim 14 , wherein the binding partner or the target analyte is a cell, bacterium, virus, prion, or phage.
Claim 19
Dependent Claim
19 words
The method according to claim 14 , wherein different binding partners are immobilised at different positions on the surface.
Claim 20
Dependent Claim
16 words
The method according to claim 14 , wherein the dissociation event is detected by acoustic emission.
Claim 21
Dependent Claim
19 words
The method according to claim 14 , wherein the surface is part of a piezoelectric transducer or acoustic transducer.
Claim 22
Dependent Claim
20 words
The method according to claim 19 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 23
Dependent Claim
14 words
The method according to claim 14 , which is carried out in a liquid.
Claim 24
Dependent Claim
26 words
The method according to claim 14 , wherein the binding partner and the target analyte are, independently, a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 25
Dependent Claim
24 words
The method according to claim 14 , wherein the binding partner and the target analyte are, independently, a cell, bacterium, virus, prion, or phage.
Claim 26
Dependent Claim
14 words
The method according to claim 14 , wherein the increasing amplitude is monotonously increasing.
Claim 27
Independent Claim
71 words
A method for detecting a target analyte in a composition, which comprises the steps of: (i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and (ii) oscillating the surface such that the amplitude increases to a value wherein the analyte dissociates from the binding partner, and wherein detection of the dissociation event is indicative of the target analyte in the composition.
Claim 28
Independent Claim
71 words
A method for detecting a target analyte in a composition, which comprises the steps of: (i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and (ii) oscillating the surface such that the amplitude increases to a value wherein the analyte dissociates from the binding partner, and wherein detection of the dissociation event is indicative of the target analyte in the composition.
Claim 29
Dependent Claim
25 words
The method according to claim 25 , wherein the binding partner or the target analyte is a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 30
Dependent Claim
23 words
The method according to claim 25 , wherein the binding partner or the target analyte is a cell, bacterium, virus, prion, or phage.
Claim 31
Dependent Claim
19 words
The method according to claim 25 , wherein different binding partners are immobilised at different positions on the surface.
Claim 32
Dependent Claim
16 words
The method according to claim 25 , wherein the dissociation event is detected by acoustic emission.
Claim 33
Dependent Claim
17 words
The method according to claim 25 , wherein the surface is a piezoelectric transducer or acoustic transducer.
Claim 34
Dependent Claim
20 words
The method according to claim 30 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 35
Dependent Claim
14 words
The method according to claim 25 , which is carried out in a liquid.
Claim 36
Dependent Claim
26 words
The method according to claim 25 , wherein the binding partner and the target analyte are, independently, a protein, antibody, antigen, enzyme, enzyme inhibitor, or polynucleotide.
Claim 37
Dependent Claim
24 words
The method according to claim 25 , wherein the binding partner and the target analyte are, independently, a cell, bacterium, virus, prion, or phage.
Claim 38
Dependent Claim
14 words
The method according to claim 25 , wherein the increasing amplitude is monotonously increasing.
References Cited (Backward Citations)
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