Abstract The present invention related to microorganisms producing taxanes, such as paclitaxel or related taxanes, to the use of said microorganisms for the production of taxanes, and to the procedure for the isolation of said microorganisms from plants. In particular, the present invention relates to microorganisms of the Actinomycetes group producing taxanes as well as to the biologically pure cultures of a strain of the Actinomycetes group which produces taxanes. The microorganisms of Actinomycetes group are preferably belonging to the suprageneric groups of Streptomycetes, Actinoplanetes, Maduromycetes, Thermomonosporas or Nocardioforms, more preferably they are belonging to genus Streptomyces, Actinoplanes, Nocardiopsis, Micromonospora, Actinomadura or Kitasatosporia, still more preferably to the genus Kitasatosporia. A particularly preferred microorganism is Kitasatosporia sp. CECT 4991.
A biologically pure culture of a microorganism belonging to the genus Kitasatosporia, which produces a taxane.
Claim 2
Dependent Claim
16 words
A biologically pure culture of a microorganism belonging to the genus Kitasatosporia, which produces a taxane.
Claim 3
Dependent Claim
33 words
The microorganism according to claim 1 which produces a taxane selected from the group consisting of paclitaxel, 10 deacetyltaxol, 7-xylosyltaxol, 7-epi-10-deacetyltaxol, 7-xylosyl-10-deacetyltaxol, 7-epi-taxol, 7-epi-10-deacetylbaccatin III, baccatin III, 10-deacetyl baccatin III, cephalomannine and 10-deacetylcephalomannine.
Claim 4
Dependent Claim
9 words
The microorganism according to claim 1 which produces paclitaxel.
Claim 5
Dependent Claim
18 words
The microorganism according to claim 1 which produces a taxane which binds to an antibody specific for paclitaxel.
Claim 6
Dependent Claim
19 words
The microorganism according to claim 1 which produces a taxane which binds to antibody R4, 8A10, 3C6 or 3H5.
Claim 7
Dependent Claim
9 words
A biologically pure culture of Kitasatosporia sp. CECT 4991.
Claim 8
Dependent Claim
9 words
A biologically pure culture of Kitasatosporia sp. CECT 4991.