Patent US6479079B1 successfully refreshed from Google Patents.
US6479079B1
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Anticalcification treatments for fixed biomaterials
Assignee: Carbomedics Inc
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Filing: 1999-12-13
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Granted: 2002-11-12
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Expires: 2019-12-13
Abstract
Abstract A method for treating biomaterial is provided in which a biological tissue, typically after being cross-linked, is contacted with an anticalcification treatment solution under condition effective to render the biomaterial resistant to in vivo calcification upon implantation in a host animal. The anticalcification treatment solutions comprise higher alcohol solutions, a polyol solutions and/or a polar aprotic organic solvent solutions.
Inventors
- 👤 Chandrashenkhar P. Pathak
- 👤 Mark A. Moore
- 👤 Richard E. Phillips
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:09
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3687; A61L27/3604; A61L27/50; A61L2400/00; A61L2400/02
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
49 words
A method for treating a biomaterial comprising contacting a biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising a compound selected from the group consisting of higher alcohols and polyols, wherein the higher alcohol or polyol comprises less than 50% by volume of said anticalcification treatment solution.
Claim 2
Independent Claim
49 words
A method for treating a biomaterial comprising contacting a biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising a compound selected from the group consisting of higher alcohols and polyols, wherein the higher alcohol or polyol comprises less than 50% by volume of said anticalcification treatment solution.
Claim 3
Dependent Claim
13 words
The method of claim 1 , wherein the biomaterial is an animal tissue.
Claim 4
Dependent Claim
14 words
The method of claim 1 , wherein the biomaterial is a cross-linked animal tissue.
Claim 5
Dependent Claim
15 words
The method of claim 1 , wherein the biomaterial is an aldehyde cross-linked animal tissue.
Claim 6
Dependent Claim
21 words
The method of claim 1 , wherein the higher alcohol or polyol is a linear or branched C4-C36 alcohol or polyol.
Claim 7
Dependent Claim
21 words
The method of claim 1 , wherein the higher alcohol or polyol is a linear or branched C6-C18 alcohol or polyol.
Claim 8
Dependent Claim
20 words
The method of claim 1 , wherein the higher alcohol or polyol is linear or branched C7-C9 alcohol or polyol.
Claim 9
Dependent Claim
15 words
The method of claim 1 , wherein the higher alcohol is heptanol, octanol, or nonanol.
Claim 10
Dependent Claim
17 words
The method of claim 1 , wherein the polyol is 1,2-octanediol, 1,8-octanediol, 1,10-decanol, 1,10-dodecanol, 1,2-dihydroxydecane or 1,2-dihydroxydodecane.
Claim 11
Dependent Claim
24 words
The method of claim 1 , wherein the higher alcohol or polyol comprises less than about 25% by volume of said anticalcification treatment solution.
Claim 12
Dependent Claim
24 words
The method of claim 1 , wherein the higher alcohol or polyol comprises less than about 10% by volume of said anticalcification treatment solution.
Claim 13
Dependent Claim
18 words
The method of claim 1 , wherein the anticalcification treatment solution further comprises at least one organic solvent.
Claim 14
Dependent Claim
28 words
The method of claim 12 , wherein the organic solvent is selected from a C 1 -C 3 alcohol, acetone, ethyl acetate, ethyl lactate, 1,4-butaenediol or polyethylene glycol.
Claim 15
Dependent Claim
18 words
The method of claim 12 , wherein the anticalcification treatment solution further comprises water or an aqueous solvent.
Claim 16
Dependent Claim
18 words
The method of claim 1 , wherein the anticalcification treatment solution further comprises water or an aqueous solvent.
Claim 17
Independent Claim
57 words
A method for treating a biomaterial comprising contacting a biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising a polar aprotic organic solvent, wherein the contacting is performed for a period of time sufficient to render the biomaterial more resistant to in vivo pathologic calcification than a biomaterial not treated with the anticalcification treatment solution.
Claim 18
Independent Claim
57 words
A method for treating a biomaterial comprising contacting a biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising a polar aprotic organic solvent, wherein the contacting is performed for a period of time sufficient to render the biomaterial more resistant to in vivo pathologic calcification than a biomaterial not treated with the anticalcification treatment solution.
Claim 19
Dependent Claim
20 words
The method of claim 16 , wherein the polar aprotic organic solvent has a dielectric constant greater than about 20.
Claim 20
Dependent Claim
20 words
The method of claim 17 , wherein the polar aprotic organic solvent has a dielectric constant greater than about 30.
Claim 21
Dependent Claim
45 words
The method of claim 4 , wherein contacting the aldehyde cross-linked biomaterial with said anticalcification treatment solution reduces the pathological calcification of the biomaterial following implantation into a mammalian host when compared to an aldehyde-cross-linked biomaterial that is not contacted with said anticalcification treatment solution.
Claim 22
Independent Claim
42 words
A method for treating a biomaterial, comprising contacting an aldehyde cross-linked biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising at least one organic solvent and from about 0.1% to about 25% by volume of a C6-C18 alcohol or polyol.
Claim 23
Independent Claim
42 words
A method for treating a biomaterial, comprising contacting an aldehyde cross-linked biomaterial with an anticalcification treatment solution, said anticalcification treatment solution comprising at least one organic solvent and from about 0.1% to about 25% by volume of a C6-C18 alcohol or polyol.
Claim 24
Dependent Claim
20 words
The method of claim 20 , wherein the C 6 -C 18 alcohol is a C 7 -C 9 alcohol.
Claim 25
Dependent Claim
18 words
The method of claim 20 , wherein the C 6 -C 18 alcohol is heptanol, octanol or nonanol.
Claim 26
Dependent Claim
21 words
The method of claim 20 , wherein the C 6 -C 18 polyol is 1,2-octanediol, 1,8-octanediol, 1,10-decanol, 1,10-dodecanol, 1,2-dihydroxydecane or 1,2-dihydroxydodecane.
Claim 27
Dependent Claim
27 words
The method of claim 20 , wherein the organic solvent is selected from C 1 -C 3 alcohol, acetone, ethyl acetate, ethyl lactate, 1,4-butaenediol or polyethylene glycol.
Claim 28
Dependent Claim
12 words
The method of claim 20 , wherein the organic solvent is ethanol.
Claim 29
Dependent Claim
18 words
The method of claim 20 , wherein the anticalcification treatment solution further comprises water or an aqueous solvent.
Claim 30
Dependent Claim
35 words
The method of claim 26 , wherein the organic solvent is present at about 35% to about 49% by volume of said anticalcification treatment solution, the remainder being comprised of said water or aqueous solvent.
Claim 31
Dependent Claim
26 words
The method of claim 26 , wherein said water or aqueous solvent is present at greater than about 50% by volume of said anticalcification treatment solution.
Claim 32
Dependent Claim
45 words
The method of claim 20 , wherein contacting the aldehyde cross-linked biomaterial with said anticalcification treatment solution reduces the pathological calcification of the biomaterial following implantation into a mammalian host when compared to an aldehyde-cross-linked biomaterial that is not contacted with said anticalcification treatment solution.
Claim 33
Independent Claim
79 words
A method for treating a biomaterial, comprising contacting an aldehyde cross-linked animal tissue with an anticalcification treatment solution comprised of about 0.1% to about 25% by volume of a C 7 -C 9 alcohol or polyol, about 25% to about 99% by volume of a C 1 -C 3 alcohol, the remaining volume, if any, being comprised of water or an aqueous solvent; under conditions effective to reduce pathologic calcification of the tissue following implantation into a mammalian host.
Claim 34
Independent Claim
79 words
A method for treating a biomaterial, comprising contacting an aldehyde cross-linked animal tissue with an anticalcification treatment solution comprised of about 0.1% to about 25% by volume of a C 7 -C 9 alcohol or polyol, about 25% to about 99% by volume of a C 1 -C 3 alcohol, the remaining volume, if any, being comprised of water or an aqueous solvent; under conditions effective to reduce pathologic calcification of the tissue following implantation into a mammalian host.
Claim 35
Dependent Claim
28 words
The method of claim 30 , wherein the C 1 -C 3 alcohol is present at about 35% to about 45% by volume of the anticalcification treatment solution.
Claim 36
Dependent Claim
30 words
The method of claim 30 , wherein the C 7 -C 9 alcohol or polyol is present at about 1% to about 10% by volume of the anticalcification treatment solution.
Claim 37
Dependent Claim
15 words
The method of claim 30 , wherein the C 7 -C 9 alcohol is octanol.
Claim 38
Dependent Claim
17 words
The method of claim 30 , wherein the C 7 -C 9 polyol is 1,2-octanediol or 1,8-octanediol.
Claim 39
Independent Claim
51 words
A method for treating a biomaterial, comprising contacting an aldehyde-cross-linked animal tissue with an anticalcification treatment solution comprising a polar aprotic organic solvent selected from the group consisting of N-methyl pyrolidinone, N,N-dimethylacetamide, N,N-dimethylformamide and N,N-dimethylpropionamide, under conditions effective to reduce pathologic calcification of the tissue following implantation into a mammalian host.
Claim 40
Independent Claim
51 words
A method for treating a biomaterial, comprising contacting an aldehyde-cross-linked animal tissue with an anticalcification treatment solution comprising a polar aprotic organic solvent selected from the group consisting of N-methyl pyrolidinone, N,N-dimethylacetamide, N,N-dimethylformamide and N,N-dimethylpropionamide, under conditions effective to reduce pathologic calcification of the tissue following implantation into a mammalian host.
Claim 41
Independent Claim
69 words
A method for treating a biomaterial, comprising contacting a cross-linked biomaterial with an anticalcification treatment solution at a temperature between about 30° and 60° C. under conditions effective to reduce pathologic calcification of the biomaterial following implantation into a mammalian host, said anticalcification treatment solution comprising between about 10% and about 50% by volume of a C1-C3 alcohol, the remaining volume being comprised of water or an aqueous buffer.
Claim 42
Independent Claim
69 words
A method for treating a biomaterial, comprising contacting a cross-linked biomaterial with an anticalcification treatment solution at a temperature between about 30° and 60° C. under conditions effective to reduce pathologic calcification of the biomaterial following implantation into a mammalian host, said anticalcification treatment solution comprising between about 10% and about 50% by volume of a C1-C3 alcohol, the remaining volume being comprised of water or an aqueous buffer.
Claim 43
Dependent Claim
13 words
The method of claim 36 , wherein the biomaterial is an animal tissue.
Claim 44
Dependent Claim
14 words
The method of claim 36 , wherein the biomaterial is a cross-linked animal tissue.
Claim 45
Dependent Claim
15 words
The method of claim 36 , wherein the biomaterial is an aldehyde cross-linked animal tissue.
Claim 46
Dependent Claim
19 words
The method of claim 36 , wherein the C 1 -C 3 alcohol is methanol, ethanol, propanol, or isopropanol.
Claim 47
Dependent Claim
12 words
The method of claim 36 , wherein the aqueous buffer comprises HEPES.
Claim 48
Dependent Claim
26 words
The method of claim 36 , wherein the C 1 -C 3 alcohol comprises about 25 to about 50% by volume of the anticalcification treatment solution.
Claim 49
Dependent Claim
28 words
The method of claim 36 , wherein the aldehyde cross-linked biomaterial is contacted with an anticalcification treatment solution at a temperature between about 30° C. and 60° C.
Claim 50
Dependent Claim
23 words
The method of claim 36 , wherein the aldehyde cross-linked biomaterial is contacted with an anticalcification treatment solution for at least 12 hours.
Claim 51
Dependent Claim
47 words
The method of claim 12 , wherein the polyol is 1,2-octanediol, the organic solvent is ethanol and the anticalcification treatment further comprises an aqueous buffer selected from the group consisting of phosphate-buffered saline, N-N-2-hydroxyethylpiperzine-N′-2-ethanesulfonic acid, morpholine propanesulphonic acid and buffers that include borate, bicarbonate, carbonate or cacodylate.
Claim 52
Dependent Claim
25 words
The method of claim 45 , wherein the anticalcification treatment comprises 40% by volume ethanol; 5% by volume 1.2-octanediol and 55% by volume N-N-2-hydroxyethylpiperzine-N′-2-ethanesulfonic buffer.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-11-11 | 2006-05-13 | 2006-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-11-11 | 2010-05-13 | 2010-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-11-11 | 2014-05-13 | 2014-11-12 | $7700.00 | Window Closed / Lapsed |