Patent US6350732B1 successfully refreshed from Google Patents.
US6350732B1
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Method for extracting lipids from tissue samples using high osmolality storage medium and product
Assignee: Carbomedics Inc
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Filing: 1997-11-17
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Granted: 2002-02-26
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Expires: 2017-11-17
Abstract
Abstract A method extracting lipids from tissue samples, particularly collagenous tissue samples for use in preserving the tissue samples, is disclosed. The product of the method of extracting the lipids is also disclosed. Extraction is accomplished by immersing the tissue sample in a medium which is an aqueous solution of a salt and a sugar that is buffered to maintain pH and which has a high (greater than about 4500 mosm) osmolality.
Inventors
- 👤 Mark A. Moore
- 👤 David T. Cheung
- 👤 Gerald L. Mechanic
- 👤 Brian K. McIlroy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:36
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3687; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; C; C08; C08H; C08H1/00; C08H1/06; A61L2430/00; A61L2430/40
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
71 words
A treatment for extracting lipids from a collagenous tissue sample comprising immersing the tissue sample in an aqueous solution of a salt and a sugar, the salt being selected from the group of salts which are capable of penetrating the sample, the sugar functioning to maintain the osmolality of the solution higher than about 4500 mosm even as the salt concentration in the solution decreases as the salt penetrates the sample.
Claim 2
Dependent Claim
71 words
A treatment for extracting lipids from a collagenous tissue sample comprising immersing the tissue sample in an aqueous solution of a salt and a sugar, the salt being selected from the group of salts which are capable of penetrating the sample, the sugar functioning to maintain the osmolality of the solution higher than about 4500 mosm even as the salt concentration in the solution decreases as the salt penetrates the sample.
Claim 3
Dependent Claim
36 words
The treatment of claim 1 wherein said salt is utilized in a weight to volume ratio of higher than about 11.7% and said sugar is utilized in a concentration of about 30 to about 80% (W/V).
Claim 4
Dependent Claim
15 words
The treatment of claim 1 wherein said salt is either sodium chloride or potassium chloride.
Claim 5
Dependent Claim
13 words
The treatment of claim 1 wherein said sugar is either sucrose or fructose.
Claim 6
Dependent Claim
19 words
The treatment of claim 1 additionally comprising maintaining the osmolality of the solution at higher than about 4500 mosm.
Claim 7
Dependent Claim
12 words
The treatment of claim 1 wherein said salt is a halide salt.
Claim 8
Dependent Claim
24 words
A tissue sample having at least a 65% lower lipid content relative to fresh tissue samples produced by the method of claim 1 .
Claim 9
Independent Claim
73 words
A method for extracting lipids from a collagenous tissue sample for the purpose of preserving the sample comprising the steps of immersing a collagenous tissue sample in an aqueous medium and maintaining the osmolality of the medium in which the sample is immersed higher than about 4500 mosm, the storage medium comprising water, about 30 to about 80% (W:V) sucrose, and a halide salt in a concentration of higher than about 11.7% (W:V).
Claim 10
Independent Claim
73 words
A method for extracting lipids from a collagenous tissue sample for the purpose of preserving the sample comprising the steps of immersing a collagenous tissue sample in an aqueous medium and maintaining the osmolality of the medium in which the sample is immersed higher than about 4500 mosm, the storage medium comprising water, about 30 to about 80% (W:V) sucrose, and a halide salt in a concentration of higher than about 11.7% (W:V).
Claim 11
Dependent Claim
16 words
The method of claim 8 wherein said halide salt is either sodium chloride or potassium chloride.
Claim 12
Dependent Claim
12 words
The method of claim 9 wherein said medium additionally comprises a buffer.
Claim 13
Dependent Claim
23 words
A tissue sample having at least a 65% lower lipid content than fresh tissue samples produced by the method of claim 8 .
Claim 14
Dependent Claim
59 words
A product obtained by immersing a collagenous tissue sample in an aqueous medium comprising water, sucrose, and a halide salt and maintaining the osmolality of the medium at higher than about 4500 mosm and characterized by the properties of having a lipid content at least 65% lower than fresh tissue samples and a lower bioburden than fresh tissue samples.
Claim 15
Dependent Claim
59 words
A product obtained by immersing a collagenous tissue sample in an aqueous medium comprising water, sucrose, and a halide salt and maintaining the osmolality of the medium at higher than about 4500 mosm and characterized by the properties of having a lipid content at least 65% lower than fresh tissue samples and a lower bioburden than fresh tissue samples.
Claim 16
Dependent Claim
29 words
The product of claim 12 wherein the medium comprises about 30 to about 80% (W:V) sucrose and a halide salt in a concentration of higher than about 11.7% (W:V).
Claim 17
Dependent Claim
12 words
The product of claim 13 wherein the medium additionally comprises a buffer.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-02-25 | 2005-08-27 | 2006-02-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-02-25 | 2009-08-27 | 2010-02-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-02-25 | 2013-08-27 | 2014-02-26 | $7700.00 | Window Closed / Lapsed |