Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids

Claim 1

A composition of matter comprising an oligonucleotide attached to a solid support, having the formula selected from the group consisting of formula (i) and formula (ii), where the symbol solid-phase represents a solid matrix to which the rest of the composition is covalently attached; n=1 to 30; m is 1 to 30; R 3 is H, C 1 -C 6 alkyl or C 3 -C 4 cycloalkyl; X is —N═, —UN═, —C═(U)—NH—N═, —NH—C═(U)—NH—N═, —NH—NH—C═(U)—NH—N═ or —U—NH—C═(U)—NH—N═, where U is independently O or S; Q is carbocyclic condensed or not-condensed aromatic ring, or a condensed or not-condensed heteroaromatic ring, said carbocyclic or heteroaromatic ring being optionally substituted with a lower alkyl, lower alkoxy or halogen group; V is a linker having the length of 2 to 100 atoms that contains carbon to carbon bonds and optionally and independently may contain carbon to oxygen bonds and one or more moieties selected from the group consisting of —NH—, —O—, —NH—C(═O)—, C═(O)—NH—,—NE—C(═O)—NH—, —NH—C(═S)—NH—, —S—, OP(O − )(O)O— and —S—S—; W is a linker having the length of 2 to 100 atoms that contains carbon to carbon bonds and optionally and independently may contain carbon to oxygen bonds and one or more moieties selected from the group consisting of —NH—, —O—, —NH—C(═O)—, —C═(O)—NH—, —NH—C(═O)—NH—, —NH—C(═S)—NH—, —S—, OP(O)(O − )O— and —S—S—, said linker W terminating with a carbon atom adjacent to X; T is a valence bond or a linker having the length of 1 to 100 atoms, that contains carbon to carbon bonds and optionally and independently may contain carbon to oxygen bonds and one or more moieties selected from the group consisting of —NH—, —O—, —NH—C(═O)—, —C═(O)—NH—, —NH—C(═O)—NH—, —NH—C(═S)—NH—, —S— and —S—S—, said linker T terminating with a carbon atom adjacent to X, R 5 is —O—P(O)(—U − )—3′-oligomer of nucleotides or —O—P═(O)(—U − )-5′-oligomer of nucleotides where U is O or S.