US6300074B1
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Systematic evolution of ligands by exponential enrichment: Chemi-SELEX
Assignee: Gilead Sciences Inc
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Filing: 1999-10-04
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Granted: 2001-10-09
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Expires: 2019-10-04
Abstract
Abstract This application provides methods for identifying nucleic acid ligands capable of covalently interacting with targets of interest. The nucleic acids can be associated with various functional units. The method also allows for the identification of nucleic acids that have facilitating activities as measured by their ability to facilitate formation of a covalent bond between the nucleic acid, including its associated functional unit, and its target.
Inventors
- 👤 Larry Gold
- 👤 Bruce Eaton
- 👤 Drew Smith
- 👤 Matthew Wecker
- 👤 Kirk Jensen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/115; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/547; A61K47/549; B; B82; B82Y; B82Y5/00; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00; C07K; C07K14/00; C07K14/001; C12N15/10; C12N15/1034; C12N15/1048; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68; C12Q1/6804; C12Q1/6811; C12Q1/6876; C12Q1/70; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/569; G01N33/56983; G01N33/56988; G01N33/68; G01N33/74; G01N33/76; C12N2310/00; C12N2310/10; C12N2310/13; C12N2310/30; C12N2310/32; C12N2310/322; C12N2310/50; C12N2310/53; C40; C40B; C40B40/00; F; F02; F02B; F02B75/00; F02B75/02; F02B2075/022; F02B2075/027; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/15; G01N2333/155; G01N2333/16; G01N2333/163; G01N2333/435; G01N2333/575; G01N2333/62; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96441; G01N2333/96455; G01N2333/966; G01N2333/972; G01N2333/9726; G01N2333/974; G01N2333/976
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
84 words
A covalent complex between a nucleic acid ligand and a target molecule, wherein said nucleic acid ligand comprises a chemically reactive functional unit, comprising: a) preparing a candidate mixture of nucleic acids comprising at least one functional unit; b) contacting said candidate mixture with said target molecule, wherein nucleic acid sequences which bind covalently with said target molecule form nucleic acid-target molecule complexes; c) partitioning the nucleic acid-target molecule complexes from the remainder of the candidate mixture, whereby a covalent complex may be identified.
Claim 2
Dependent Claim
84 words
A covalent complex between a nucleic acid ligand and a target molecule, wherein said nucleic acid ligand comprises a chemically reactive functional unit, comprising: a) preparing a candidate mixture of nucleic acids comprising at least one functional unit; b) contacting said candidate mixture with said target molecule, wherein nucleic acid sequences which bind covalently with said target molecule form nucleic acid-target molecule complexes; c) partitioning the nucleic acid-target molecule complexes from the remainder of the candidate mixture, whereby a covalent complex may be identified.
Claim 3
Dependent Claim
22 words
The covalent complex of claim 1 wherein said nucleic acid ligand is selected from the sequences listed in Tables II and IV.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-10-08 | 2005-04-09 | 2005-10-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-10-08 | 2009-04-09 | 2009-10-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-10-08 | 2013-04-09 | 2013-10-09 | $7700.00 | Window Closed / Lapsed |