US5763595A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Systematic evolution of ligands by exponential enrichment: Chemi-SELEX
Assignee: Gilead Sciences Inc
•
Filing: 1995-06-05
•
Granted: 1998-06-09
•
Expires: 2015-06-05
Abstract
Abstract This application provides methods for identifying nucleic acid ligands capable of covalently interacting with targets of interest. The nucleic acids can be associated with various functional units. The method also allows for the identification of nucleic acids that have facilitating activities as measured by their ability to facilitate formation of a covalent bond between the nucleic acid, including its associated functional unit, and its target.
Inventors
- 👤 Larry Gold
- 👤 Bruce Eaton
- 👤 Drew Smith
- 👤 Matthew Wecker
- 👤 Kirk Jensen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/547; A61K47/549; B; B82; B82Y; B82Y5/00; C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00; C07K; C07K14/00; C07K14/001; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1048; C12N15/11; C12N15/115; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68; C12Q1/6811; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/569; G01N33/56983; G01N33/56988; G01N33/68; C12N2310/00; C12N2310/10; C12N2310/13; C12N2310/30; C12N2310/32; C12N2310/322; C12N2310/50; C12N2310/53; C40; C40B; C40B40/00; F; F02; F02B; F02B75/00; F02B75/02; F02B2075/022; F02B2075/027; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/15; G01N2333/155; G01N2333/16; G01N2333/163; G01N2333/435; G01N2333/575; G01N2333/62; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96436; G01N2333/96441; G01N2333/96455; G01N2333/96486; G01N2333/966; G01N2333/972; G01N2333/9726; G01N2333/974; G01N2333/976
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
109 words
Nucleic acid ligands which bind covalently with a protein, wherein said nucleic acid ligands comprise a chemically reactive functional unit selected from the group consisting of pohotoreactive groups and active site directed compounds, wherein said nucleic acid ligands are produced by a method comprising the steps of: a) preparing a candidate mixture of nucleic acids; b) contacting said candidate mixture with said protein, wherein nucleic acids which bind covalently with said protein may be partitioned from the remainder of said candidate mixture; c) partitioning the nucleic acid-protein complexes from free nucleic acids in said candidate mixture; and d) identifying said nucleic acid ligands that bind covalently with said protein.
Claim 2
Dependent Claim
109 words
Nucleic acid ligands which bind covalently with a protein, wherein said nucleic acid ligands comprise a chemically reactive functional unit selected from the group consisting of pohotoreactive groups and active site directed compounds, wherein said nucleic acid ligands are produced by a method comprising the steps of: a) preparing a candidate mixture of nucleic acids; b) contacting said candidate mixture with said protein, wherein nucleic acids which bind covalently with said protein may be partitioned from the remainder of said candidate mixture; c) partitioning the nucleic acid-protein complexes from free nucleic acids in said candidate mixture; and d) identifying said nucleic acid ligands that bind covalently with said protein.
Claim 3
Dependent Claim
19 words
The nucleic acid ligands of claim 1 which are selected from the sequences listed in Tables II and IV.
Claim 4
Dependent Claim
16 words
The nucleic acid ligand of claim 1 wherein said nucleic acids comprise a fixed sequence region.
Claim 5
Dependent Claim
24 words
The nucleic acid ligand of claim 3 wherein at least one fuinctional unit is attached to an oligonucleotide hybridized to said fixed sequence region.
Claim 6
Dependent Claim
37 words
A purified and non-naturally occurring nucleic acid ligand which binds covalently with a protein, wherein said nucleic acid ligand comprises a chemically reactive functional unit selected from the group comprising photoreactive groups and active site directed compounds.
Claim 7
Dependent Claim
37 words
A purified and non-naturally occurring nucleic acid ligand which binds covalently with a protein, wherein said nucleic acid ligand comprises a chemically reactive functional unit selected from the group comprising photoreactive groups and active site directed compounds.
References Cited (Backward Citations)
US4959309A
Cited
US4808520A
Cited
WO1991014696A1
Cited
US5270163A
Cited
WO1992014843A1
Cited
WO1994001448A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-08 | 2001-12-08 | 2002-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-08 | 2005-12-08 | 2006-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-08 | 2009-12-08 | 2010-06-09 | $7700.00 | Window Closed / Lapsed |