US6117398A
Expired / Lapsed
US
⏱️ 0.0 years remaining
System for releasing and isolating nucleic acids
Assignee: Roche Diagnostics GmbH
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Filing: 1996-03-28
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Granted: 2000-09-12
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Expires: 2016-03-28
Abstract
Abstract PCT No. PCT/EP96/01368 Sec. 371 Date Feb. 17, 1998 Sec. 102(e) Date Feb. 17, 1998 PCT Filed Mar. 28, 1996 PCT Pub. No. WO96/31781 PCT Pub. Date Oct. 10, 1996An instrument that can hold one or more sample processsing vessels, maintain the sample processing vessels at a constant temperature, shake the sample processing vessels and separate magnetic particles by means of magnetic force. This system greatly simplifies the isolation of nucleic acids.
Inventors
- 👤 Gerhard Bienhaus
- 👤 Ulrich Schubert
- 👤 Uwe Kolb
- 👤 Burkhard Stolz
- 👤 Manfred Pasch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 13:47
CPC Classifications
G; G01; G01N; G01N35/00; G01N35/0098; C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N15/1013; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
136 words
A system, comprising a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments; a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening; a plurality of magnetic particles for introducing into the sample processing vessel, wherein said magnetic particles are capable of binding with the biological compartments; a thermostat unit configured to maintain the sample processing vessel and its contents at an essentially constant temperature; a mechanical shaker configured to shake the sample processing vessel; and a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel, wherein said receptacle, said thermostat unit and said magnetic means are integrated into a reaction block.
Claim 2
Independent Claim
136 words
A system, comprising a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments; a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening; a plurality of magnetic particles for introducing into the sample processing vessel, wherein said magnetic particles are capable of binding with the biological compartments; a thermostat unit configured to maintain the sample processing vessel and its contents at an essentially constant temperature; a mechanical shaker configured to shake the sample processing vessel; and a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel, wherein said receptacle, said thermostat unit and said magnetic means are integrated into a reaction block.
Claim 3
Dependent Claim
13 words
The system of claim 1, wherein said sample processing vessel contains biological compartments.
Claim 4
Dependent Claim
16 words
The system of claim 1, further comprising a hose which is connected to said hose connection.
Claim 5
Dependent Claim
21 words
The system of claim 1, further comprising a hose, wherein one end of said hose is connected to said hose connection.
Claim 6
Dependent Claim
22 words
The system of claim 1, wherein said magnetic means and said receptacle are configured to move towards or away from one another.
Claim 7
Dependent Claim
25 words
The system of claim 1, wherein said receptacle defines at least two cavities, wherein each cavity is configured to hold therein a sample processing vessel.
Claim 8
Dependent Claim
24 words
The system of claim 6, further comprising another sample processing vessel, wherein each of the sample processing vessels is held in a corresponding cavity.
Claim 9
Dependent Claim
15 words
The system of claim 6, wherein the cavities are arranged in an essentially linear format.
Claim 10
Dependent Claim
13 words
The system of claim 1, wherein said mechanical shaker comprises an eccentric drive.
Claim 11
Dependent Claim
57 words
The system of claim 1, wherein said thermostat unit comprises a thermally conductive metal block which is integrated into said receptacle, and the at least one cavity is configured to snugly hold therein the sample processing vessel such that said metal block is capable of efficiently transferring heat between said metal block and the sample processing vessel.
Claim 12
Dependent Claim
18 words
The system of claim 1, wherein said thermostat unit comprises a resistance heating unit and a peltier element.
Claim 13
Dependent Claim
20 words
The system of claim 1, wherein said sample processing vessel and the at least one cavity have essentially matching conicity.
Claim 14
Dependent Claim
18 words
The system of claim 1, wherein said thermostat unit regulates the temperature by means of a fuzzy regulator.
Claim 15
Dependent Claim
21 words
The system of claim 1, wherein said thermostat unit regulates the temperature by means of a proportion integral differential time regulator.
Claim 16
Dependent Claim
18 words
The system of claim 1, wherein said thermostat unit comprises a block through which a thermostating medium flows.
Claim 17
Dependent Claim
12 words
The system of claim 1, wherein said magnetic particles comprise ferromagnetic particles.
Claim 18
Dependent Claim
34 words
The system of claim 1, further comprising a pump unit which is connected to said hose connection, wherein said pump unit is configured to remove the fluid from the outlet opening through the hose.
Claim 19
Independent Claim
134 words
A system, comprising a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments; a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening; a plurality of magnetic particles for introducing into the sample processing vessel, wherein the magnetic particles are capable of binding with the biological compartments; a thermostat means for maintaining the sample processing vessel and its contents at an essentially constant temperature; a shaking means for shaking the sample processing vessel; and a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel, wherein said receptacle, said thermostat means and said magnetic means are integrated into a reaction block.
Claim 20
Independent Claim
134 words
A system, comprising a sample processing vessel having an outlet opening and being capable of containing fluid and biological compartments; a receptacle defining at least one cavity which holds therein the sample processing vessel, wherein said receptacle comprises a hose connection for connecting a hose to the outlet opening; a plurality of magnetic particles for introducing into the sample processing vessel, wherein the magnetic particles are capable of binding with the biological compartments; a thermostat means for maintaining the sample processing vessel and its contents at an essentially constant temperature; a shaking means for shaking the sample processing vessel; and a magnetic means for magnetically attracting said magnetic particles to an inner wall of the sample processing vessel, wherein said receptacle, said thermostat means and said magnetic means are integrated into a reaction block.
Claim 21
Dependent Claim
34 words
The system of claim 18, further comprising a pump unit which is connected to said hose connection, wherein said pump unit is configured to remove the fluid from the outlet opening through the hose.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-09-12 | 2004-03-13 | 2004-09-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-09-12 | 2008-03-13 | 2008-09-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-09-12 | 2012-03-13 | 2012-09-12 | $7700.00 | Window Closed / Lapsed |