US6086913A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Liposomal delivery of AAV vectors
Assignee: University of British Columbia
•
Filing: 1996-10-25
•
Granted: 2000-07-11
•
Expires: 2016-10-25
Abstract
Abstract The invention provides compositions and methods for introducing a nucleic acid fragment into the genome of a cell. Suitable compositions comprise an expression vector having first and second inverted repeated sequences from an adeno associated virus, a rep gene from an adeno associated virus and the nucleic acid fragment. The expression vector is complexed with lipids.
Inventors
- 👤 Patrick Tam
- 👤 Arcadio Chonn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:01
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61K; A61K48/00; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; C12N15/87; C12N15/88; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14111; C12N2750/14141; C12N2750/14143
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
54 words
A serum-stable composition for introducing a nucleic acid fragment into the genome of a cell, comprising: an expression vector comprising first and second inverted repeated sequences from an adeno associated virus, a rep gene from an adeno associated virus and the nucleic acid fragment; and wherein the expression vector is in a lipid formulation.
Claim 2
Dependent Claim
54 words
A serum-stable composition for introducing a nucleic acid fragment into the genome of a cell, comprising: an expression vector comprising first and second inverted repeated sequences from an adeno associated virus, a rep gene from an adeno associated virus and the nucleic acid fragment; and wherein the expression vector is in a lipid formulation.
Claim 3
Dependent Claim
38 words
The composition of claim 1, wherein the expression vector further comprises at least one gene contained within an adenovirus DNA segment wherein said gene is a member selected from the group consisting of E1, E2, E3 and E4.
Claim 4
Dependent Claim
19 words
The composition of claim 2, wherein the expression vector comprises said adenovirus DNA segments E1, E2, E3 and E4.
Claim 5
Dependent Claim
21 words
The composition of claim 1, wherein the nucleic acid fragment encodes a protein coding sequence in operable linkage to a promoter.
Claim 6
Dependent Claim
14 words
The composition of claim 1, wherein the nucleic acid fragment encodes an antisense transcript.
Claim 7
Dependent Claim
95 words
The composition of claim 1, wherein the lipid formulation comprises lipids complexed with the vector by a) combining the vector with cationic lipids in a detergent solution to provide a coated vector-lipid complex; b) contacting noncationic lipids with the coated vector-lipid complex to provide a solution comprising detergent, a vector-lipid complex and noncationic lipids; and c) removing the detergent from the solution of step b to provide a solution of serum-stable plasmid-lipid particles, wherein the plasmid is encapsulated in a lipid bilayer and the particles are serum-stable and have a size from about 50-150 nm.
Claim 8
Dependent Claim
81 words
The composition of claim 1, wherein the lipid formulation comprises lipids complexed with the vector by preparing a mixture comprising cationic lipids and noncationic lipids in an organic solvent; contacting an aqueous solution of said vector with the mixture to provide a clear single phase and removing the organic solvent to provide a suspension of plasmid-lipid particles, wherein the plasmid is encapsulated in a lipid bilayer, and the particles are stable in serum and have a size of about 50-150 nm.
Claim 9
Dependent Claim
13 words
The composition of claim 1, wherein the vector further comprises a loxP site.
Claim 10
Dependent Claim
17 words
The composition of claim 1, wherein the vector further comprises a DNA segment encoding a selection marker.
Claim 11
Dependent Claim
15 words
The composition of claim 1, wherein the nucleic acid fragment is greater than 5 kb.
Claim 12
Dependent Claim
10 words
The composition of claim 1, further comprising a pharmaceutical excipient.
Claim 13
Dependent Claim
13 words
The composition of claim 1, wherein the lipid formulation is a lipid complex.
Claim 14
Dependent Claim
18 words
The composition of claim 8, further comprising a Cre enzyme or a DNA segment encoding a Cre enzyme.
References Cited (Backward Citations)
US4797368A
Cited
US5139941A
Cited
US5436146A
Cited
US5264618A
Cited
WO1991018088A1
Cited
US5354678A
Cited
US5587308A
Cited
WO1995006743A2
Cited
WO1995007995A2
Cited
WO1995013392A1
Cited
WO1995013365A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-07-11 | 2004-01-10 | 2004-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-07-11 | 2008-01-10 | 2008-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-07-11 | 2012-01-10 | 2012-07-11 | $7700.00 | Window Closed / Lapsed |