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US6045996A
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Hybridization assays on oligonucleotide arrays
Assignee: Affymetrix Inc
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Filing: 1996-05-16
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Granted: 2000-04-04
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Expires: 2016-05-16
Abstract
Abstract This invention provides methods of performing nucleic acid hybridization assays on high-density substrate-bound oligonucleotide arrays involving including in the hybridization mixture an isostabilizing agent, a denaturing agent or a renaturation accelerant.
Inventors
- 👤 Maureen T. Cronin
- 👤 Charles Garrett Miyada
- 👤 Mark Trulson
- 👤 Thomas R. Gingeras
- 👤 Glenn McGall
- 👤 Claire Robinson
- 👤 Michelle Oval
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:34
CPC Classifications
B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y30/00; C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/6832; C12Q1/6834; C12Q1/6837; C12Q1/6869; C12Q1/6874; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00427; B01J2219/00432; B01J2219/00497; B01J2219/00527; B01J2219/00529; B01J2219/00583; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/0061; B01J2219/00612; B01J2219/00623; B01J2219/00626; B01J2219/00659; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; C07B; C07B2200/00; C07B2200/11; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
84 words
A method for performing a hybridization assay between a target nucleic acid molecule and an oligonucleotide array, the array comprising a surface to which are covalently attached oligonucleotide probes with different, known sequences, at discrete, known locations, the array having a density of at least 500 said known locations of said different known sequences per square cm, the method comprising incubating the array with a hybridization mixture comprising the target and betaine and determining the identity of probes to which the target has hybridized.
Claim 2
Independent Claim
84 words
A method for performing a hybridization assay between a target nucleic acid molecule and an oligonucleotide array, the array comprising a surface to which are covalently attached oligonucleotide probes with different, known sequences, at discrete, known locations, the array having a density of at least 500 said known locations of said different known sequences per square cm, the method comprising incubating the array with a hybridization mixture comprising the target and betaine and determining the identity of probes to which the target has hybridized.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the mixture comprises betaine in a concentration of about 1 M to about 10 M.
Claim 4
Dependent Claim
21 words
The method of claim 2 wherein the mixture comprises betaine in a concentration of about 4 M to about 6 M.
Claim 5
Dependent Claim
23 words
The method of claim 1 further comprising the step, after incubating the array, of washing the array with a solution that comprises betaine.
Claim 6
Dependent Claim
13 words
The method of claim 1 wherein the target further comprises a detectable label.
Claim 7
Dependent Claim
12 words
The method of claim 5 wherein the label is a fluorescent label.
Claim 8
Dependent Claim
11 words
The method of claim 6 wherein the fluorescent label is fluorescein.
Claim 9
Dependent Claim
20 words
The method of claim 1 wherein the array has a density of at least ten thousand features per square cm.
Claim 10
Dependent Claim
21 words
The method of claim 8 wherein the array has a density of at least one hundred thousand features per square cm.
Claim 11
Dependent Claim
20 words
The method of claim 9 wherein the array has a density of at least one million features per square cm.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-04-04 | 2003-10-04 | 2004-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-04-04 | 2007-10-04 | 2008-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-04-04 | 2011-10-04 | 2012-04-04 | $7700.00 | Window Closed / Lapsed |