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US6040191A
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Raman spectroscopic method for determining the ligand binding capacity of biologicals
Assignee: Individual
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Filing: 1998-10-22
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Granted: 2000-03-21
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Expires: 2018-10-22
Abstract
Abstract A nondestructive process for determining the reactive capacity of a test biological by Raman scattering. The test biological may be any one of enzymes, enzyme cofactors, coenzymes, antibodies, antibody fragments, hemeproteins, peptides, synthetic peptides, toxins, toxoids, glycosphingolipids, lectins, lipids, lipid complexes, phospholipids, carbohydrates, saccharides, gangliosides, nucleic acids, fragments of nucleic acids, pathogen adhesion factors, receptors, receptor subunits, membranes, organelles, cells, tissues and complexes containing membranes, organelles, cells and tissues, or a bioconcentrator. The test biological is irradiated with a light source to produce a Raman scattering spectrum of the irradiated biological. The Raman scattering spectrum is collected and processed to determine the ability of the test biological to react with ligands. The analyzing step includes comparing the Raman scattering spectrum of the test biological against that of a biological standard of the same biological which has been altered to vary the capability to react with ligands thereby determining the capacity of the test biological to react with ligands.
Inventors
- 👤 Ann E. Grow
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:07
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/84; G01N21/8483; G01N21/62; G01N21/63; G01N21/65; G01N35/00; G01N35/00009; G01N2021/653; G01N2021/655; G01N2021/656; G01N21/658
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
125 words
A nondestructive process for determining the capability of a test biological to bond with at least one ligand comprising the steps of irradiating a test biological with a light source capable of producing Raman scattering to produce a Raman scattering spectrum of the irradiated test biological, collecting and processing the Raman scattering spectrum, and analyzing the processed Raman scattering spectrum to determine the capability of the test biological to react with ligands, whereby said analyzing step includes the step of comparing the Raman scattering spectrum of the test biological so obtained for the test biological against the Raman scattering spectrum correspondingly obtained from at least one standard sample of the same type of biological, said standard biological sample having a known ability to bind ligands.
Claim 2
Dependent Claim
125 words
A nondestructive process for determining the capability of a test biological to bond with at least one ligand comprising the steps of irradiating a test biological with a light source capable of producing Raman scattering to produce a Raman scattering spectrum of the irradiated test biological, collecting and processing the Raman scattering spectrum, and analyzing the processed Raman scattering spectrum to determine the capability of the test biological to react with ligands, whereby said analyzing step includes the step of comparing the Raman scattering spectrum of the test biological so obtained for the test biological against the Raman scattering spectrum correspondingly obtained from at least one standard sample of the same type of biological, said standard biological sample having a known ability to bind ligands.
Claim 3
Dependent Claim
61 words
A nondestructive process as set forth in claim 1 wherein said test biological is selected from the group consisting of: enzymes, enzyme cofactors, coenzymes, antibodies, hemeproteins, peptides, synthetic peptides, toxins, toxoids, glycosphingolipids, lectins, lipids, phospholipids, carbohydrates, saccharides, gangliosides, nucleic acids, pathogen adhesion factors, receptors, receptor subunits, membranes, organelles and cells, indentifiable fragments of these biologicals, and combination thereof containing these biologicals.
Claim 4
Dependent Claim
48 words
A nondestructive process as set forth in claim 1 wherein the ability of said test biological to bind with ligands may have been altered by any one of synthesis, extraction, separation, purification, lyophilization, crystallization, reconstitution, blending, digestion, fragmentation, derivitization, heating, irradiation, aging, complex formation, chemical modification and immobilization.
Claim 5
Dependent Claim
17 words
A nondestructive process as set forth in claim 1 wherein the full Raman scattering spectrum is analyzed.
Claim 6
Dependent Claim
22 words
A nondestructive process as set forth in claim 1 wherein at least a select band of the Raman scattering spectrum is analyzed.
Claim 7
Dependent Claim
26 words
A nondestructive process as set forth in claim 1 wherein said step of collecting and processing the Raman scattering spectrum is performed by a Raman spectrometer.
Claim 8
Dependent Claim
27 words
A nondestructive process as set forth in claim 1 wherein the produced Raman spectrum scattering from is not produced by a reporter attached to said test biological.
Claim 9
Dependent Claim
70 words
A nondestructive process as set forth in claim 1 wherein said collecting and processing step is carried out by a spectrometer selected from the group consisting of: dispersing spectrometers, multichannel spectrometers, Fourier transform spectrometers, Hadamard transform spectrometers, stationary transform spectrometers, acousto-optic tunable filter spectrometers, integrated optic acousto-optic tunable filter spectrometers, fiber optic spectrometers, fiber optic array spectrometers, microscope spectrometers, imaging spectrometers, liquid crystal tunable filter spectrometers and imaging microscope spectrometers.
Claim 10
Dependent Claim
40 words
A nondestructive process as set forth in claim 1 wherein said Raman scattering is selected from the group consisting of: normal Raman scattering, surface enhanced Raman scattering, resonance Raman scattering, surface enhanced resonance Raman scattering and Raman evanescent wave scattering.
Claim 11
Dependent Claim
31 words
A nondestructive process as set forth in claim 1 wherein the test biological is a bioconcentrator and wherein said analyzing step determines whether the bioconcentrator has become at least partially deactivated.
Claim 12
Dependent Claim
152 words
A nondestructive process as set forth in claim 10 wherein the bioconcentrator is located on a support member and wherein the bioconcentrator is selected from the group consisting of: molecules, macromolecules, complexes, fragments of a molecule, and fragments and subunits of a complex, all derived from a biological source; ligands that will bind molecules, macromolecules, complexes, fragments of a molecule, subunits of a complex, and fragments of a complex derived from a biological source; said molecules, macromolecules and complexes being selected from the group consisting of enzymes, enzyme cofactors, coenzymes, antibodies, antibody fragments, hemeproteins, natural and synthetic peptides, glycosphingolipids, lectins, lipids, phospholipids, carbohydrates, saccharides, gangliosides, nucleic acids, fragments of nucleic acids, pathogen adhesion factors, receptors, receptor subunits, liposomes, membranes, organelles, cells, tissues, complexes containing said molecules or macromolecules, and said ligands being selected from the group consisting of enzyme substrates, enzyme inhibitors, antigens, antigen analogues, haptens, receptor agonists, receptor antagonists and sugars.
References Cited (Backward Citations)
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