US6040166A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Kits for amplifying and detecting nucleic acid sequences, including a probe
Assignee: Roche Molecular Systems Inc
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Filing: 1994-09-27
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Granted: 2000-03-21
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Expires: 2014-09-27
Abstract
Abstract The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
Inventors
- 👤 Henry A. Erlich
- 👤 Glenn Horn
- 👤 Randall K. Saiki
- 👤 Kary B. Mullis
- 👤 David H. Gelfand
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 05:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6858; B; B01; B01J; B01J19/00; B01J19/0046; B01L; B01L7/00; B01L7/52; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/70539; C07K14/795; C07K14/805; C12N; C12N15/00; C12N15/09; C12N15/10; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12Q1/6813; C12Q1/6827; C12Q1/6853; C12Q1/686; B01J2219/00; B01J2219/00274; B01J2219/00583; B01J2219/0059; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; Y; Y10; Y10S; Y10S977/00; Y10S977/902; Y10S977/904; Y10S977/92
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
48 words
A kit for the amplification of a nucleic acid, said kit comprising: (a) first and second oligonucleotide primers, said oligonucleotide primers differing in sequence from each other; (b) an oligonucleotide probe that differs in sequence from said first and second oligonucleotide primers; and (c) a DNA polymerase enzyme.
Claim 2
Dependent Claim
48 words
A kit for the amplification of a nucleic acid, said kit comprising: (a) first and second oligonucleotide primers, said oligonucleotide primers differing in sequence from each other; (b) an oligonucleotide probe that differs in sequence from said first and second oligonucleotide primers; and (c) a DNA polymerase enzyme.
Claim 3
Dependent Claim
17 words
The kit according to claim 1 wherein said oligonucleotide probe is labeled with a detectable label moiety.
Claim 4
Dependent Claim
13 words
The kit according to claim 2 which further comprises four different nucleoside triphosphates.
Claim 5
Dependent Claim
13 words
The kit according to claim 1 which further comprises four different nucleoside triphosphates.
Claim 6
Dependent Claim
19 words
The kit according to claim 1 in which said two oligonucleotide primers each contain about 15 to 25 nucleotides.
Claim 7
Dependent Claim
22 words
The kit according to claim 1 in which at least one of said first and second oligonucleotide primers contains a restriction site.
Claim 8
Dependent Claim
22 words
The kit according to claim 1 in which said first and second oligonucleotide primers have the nucleic acid sequences ACACAACTGTGTTCACTAGC and CAACTTCATCCACGTTCACC.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-03-21 | 2003-09-20 | 2004-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-03-21 | 2007-09-20 | 2008-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-03-21 | 2011-09-20 | 2012-03-21 | $7700.00 | Window Closed / Lapsed |