Patent US5993434A successfully refreshed from Google Patents.
US5993434A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of treatment using electroporation mediated delivery of drugs and genes
Assignee: University of South Florida Research Foundation Inc
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Filing: 1995-09-29
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Granted: 1999-11-30
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Expires: 2015-09-29
Abstract
Abstract A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.
Inventors
- 👤 Sukhendu B. Dev
- 👤 Gunter A. Hofmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:46
CPC Classifications
A; A61; A61N; A61N1/00; A61N1/18; A61N1/32; A61N1/327; A61N1/325; C; C12; C12M; C12M35/00; C12M35/02
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
150 words
A method for the therapeutic application of electroporation to a portion of the body of a patient for introducing molecules into tissues therein, comprising: providing an array of multiple opposed pairs of electrodes connected to a generator, wherein at least two pairs of electrodes are activated simultaneously, at least one of said pairs of electrodes having a needle configuration for penetrating tissue; inserting a first pair of said needle electrodes into selected tissue for introducing molecules into the tissue; positioning a second pair of electrodes of said array of electrodes in conductive relation to said selected tissue so that said tissue is between said first and second opposed pairs of electrodes; and separately applying pulses of high amplitude electric signals to at least two different pairs of electrodes, but not all electrodes simultaneously and proportionately to the distance between said pairs of electrodes causing in vivo electroporation of the tissue.
Claim 2
Independent Claim
150 words
A method for the therapeutic application of electroporation to a portion of the body of a patient for introducing molecules into tissues therein, comprising: providing an array of multiple opposed pairs of electrodes connected to a generator, wherein at least two pairs of electrodes are activated simultaneously, at least one of said pairs of electrodes having a needle configuration for penetrating tissue; inserting a first pair of said needle electrodes into selected tissue for introducing molecules into the tissue; positioning a second pair of electrodes of said array of electrodes in conductive relation to said selected tissue so that said tissue is between said first and second opposed pairs of electrodes; and separately applying pulses of high amplitude electric signals to at least two different pairs of electrodes, but not all electrodes simultaneously and proportionately to the distance between said pairs of electrodes causing in vivo electroporation of the tissue.
Claim 3
Dependent Claim
30 words
The method of claim 1 comprising the step of providing a switch assembly including a rotary switch selectively positionable for connecting alternate opposite pairs of electrodes to a pulse generator.
Claim 4
Dependent Claim
23 words
The method of claim 1 wherein the molecule is selected from the group consisting of a chemotherapeutic agent, a polynucleotide and a polypeptide.
Claim 5
Dependent Claim
11 words
The method of claim 3, wherein the chemotherapeutic agent is bleomycin.
Claim 6
Dependent Claim
22 words
The method of claim 1 wherein the molecule is introduced by a method selected fromthe group consisting of intratumorally, systemically and locally.
Claim 7
Dependent Claim
27 words
The method of claim 1, wherein the tissue is selected from the group consisting of pancreas, lung, heart, kidney, muscle, breast, colon, prostate, thymus, testis, and ovary.
Claim 8
Independent Claim
157 words
A method for the in vivo electroporation-mediated therapy of a cell of a subject comprising: administering a therapeutic composition to the subject such that the composition is delivered to the cell; providing an array of multiple opposed pairs of electrodes connected to a generator, wherein at least two pairs of electrodes are activated simultaneously, at least one of said pairs of electrodes having a needle configuration for penetrating tissue; inserting a first pair of said needle electrodes into selected tissue for introducing molecules into the cell; positioning a second pair of electrodes of said array of electrodes in conductive relation to said selected tissue so that said cell is between said first and second opposed pairs of electrodes; and separately applying electrical pulses of short duration to at least two different pairs of electrodes, but not all electrodes simultaneously and proportionately to the distance between said electrodes causing in vivo electroporation of a cell of the tissue.
Claim 9
Independent Claim
157 words
A method for the in vivo electroporation-mediated therapy of a cell of a subject comprising: administering a therapeutic composition to the subject such that the composition is delivered to the cell; providing an array of multiple opposed pairs of electrodes connected to a generator, wherein at least two pairs of electrodes are activated simultaneously, at least one of said pairs of electrodes having a needle configuration for penetrating tissue; inserting a first pair of said needle electrodes into selected tissue for introducing molecules into the cell; positioning a second pair of electrodes of said array of electrodes in conductive relation to said selected tissue so that said cell is between said first and second opposed pairs of electrodes; and separately applying electrical pulses of short duration to at least two different pairs of electrodes, but not all electrodes simultaneously and proportionately to the distance between said electrodes causing in vivo electroporation of a cell of the tissue.
Claim 10
Dependent Claim
30 words
The method of claim 7 comprising the step of providing a switch assembly including a rotary switch selectively positionable for connecting alternate opposite pairs of electrodes to a pulse generator.
Claim 11
Dependent Claim
24 words
The method of claim 7 wherein the therapeutic composition is selected from the group consisting of a chemotherapeutic agent, a polynucleotide and a polypeptide.
Claim 12
Dependent Claim
23 words
The method of claim 7 wherein the therapeutic composition is introduced by a method selected fromthe group consisting of intratumorally, systemically and locally.
Claim 13
Dependent Claim
11 words
The method of claim 9, wherein the chemotherapeutic agent is bleomycin.
Claim 14
Dependent Claim
27 words
The method of claim 7, wherein the tissue is selected from the group consisting of pancreas, lung, heart, kidney, muscle, breast, colon, prostate, thymus, testis, and ovary.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-11-29 | 2003-05-31 | 2003-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-11-29 | 2007-05-31 | 2007-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-11-29 | 2011-05-31 | 2011-11-30 | $7700.00 | Window Closed / Lapsed |