US5976833A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for animal cell culture
Assignee: Daiichi Sankyo Co Ltd
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Filing: 1996-09-19
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Granted: 1999-11-02
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Expires: 2016-09-19
Abstract
Abstract The object of the present invention is to provide a method for improving productivity in the production of useful substances by animal cells. The present invention discloses a method for animal cell culture to produce a desired substance, comprising the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.
Inventors
- 👤 Kazuaki Furukawa
- 👤 Kazuhiro Ohsuye
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 15:42
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N5/00; C12P; C12P21/00; C12P21/02; C12N2523/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
68 words
A method for culturing recombinant animal cells, transformed or transfected with a vector prepared by in vitro gene recombination, wherein said recombinant animal cells are not temperature sensitive mutants, to increase production of a desired product, comprising the steps of: (1) culturing the recombinant animal cells at a temperature at which the animal cells can grow; and then (2) culturing the recombinant animal cells at a lower temperature.
Claim 2
Independent Claim
68 words
A method for culturing recombinant animal cells, transformed or transfected with a vector prepared by in vitro gene recombination, wherein said recombinant animal cells are not temperature sensitive mutants, to increase production of a desired product, comprising the steps of: (1) culturing the recombinant animal cells at a temperature at which the animal cells can grow; and then (2) culturing the recombinant animal cells at a lower temperature.
Claim 3
Dependent Claim
38 words
A method according to claim 1 wherein said recombinant animal cells are recombinant cells that exhibit greater productivity of said desired product at the lower culturing temperature than at the temperature at which the animal cells can grow.
Claim 4
Dependent Claim
18 words
A method according to claim 2 wherein said recombinant animal cells are recombinant cells derived from homeothermic animals.
Claim 5
Dependent Claim
17 words
A method according to claim 3 wherein said recombinant animal cells are recombinant cells derived from mammals.
Claim 6
Dependent Claim
17 words
A method according to claim 4 wherein said recombinant animal cells are recombinant cells derived from rodents.
Claim 7
Dependent Claim
22 words
A method according to claim 5 wherein said recombinant animal cells are recombinant cells derived from CHO (Chinese hamster ovary) cell line.
Claim 8
Dependent Claim
22 words
A method according to claim 6 wherein said animal cells are recombinant cells established by the DHFR (dihydrofolate reductase) gene amplification system.
Claim 9
Dependent Claim
18 words
A method according to claim 7 wherein said animals cells are recombinant CHO cells that produce amidating enzymes.
Claim 10
Dependent Claim
17 words
A method according to claim 1, wherein said lowered culturing temperature is less than about 37° C.
Claim 11
Dependent Claim
23 words
A method according to claim 1, wherein said temperature at which the animal cells can grow is between about 36 and 38° C.
Claim 12
Dependent Claim
16 words
A method according to claim 10 wherein said lowered culturing temperature is 30 to 35° C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-11-01 | 2003-05-03 | 2003-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-11-01 | 2007-05-03 | 2007-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-11-01 | 2011-05-03 | 2011-11-02 | $7700.00 | Window Closed / Lapsed |