EP0501692A2
Expired / Lapsed
EP
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Fermentation processes for the production of peptides by a host
Assignee: AstraZeneca AB
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Filing: 1992-02-21
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Granted: —
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Expires: 2012-02-21
Abstract
Abstract Processes for preparing polypeptides such as ricin A such that soluble polypeptide may be obtained. The pH may be adjusted during the process or the temperature decreased during the terminal portion of the cultivation, and yeast extract may be added to the growth medium during the cultivation. In the case of ricin A the pH is generally adjusted by lowering the pH from a first value such as 6.7 to a second value such as a value between about 5.5 and 6.7.
Inventors
- 👤 Bhuphendra Vallabh Kara
- 👤 Robert Craig Hockney
- 👤 John Edward Fitton
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 15:56
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/415; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12P; C12P21/00; C12P21/02
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
77 words
A process for preparing a polypeptide, which process comprises cultivating a host capable of expressing said polypeptide in a growth medium and:- (a) adjusting the pH of the growth medium during the process, and optionally reducing the temperature of the growth medium during the process; or (b) maintaining the pH at a value which favours growth of the host, and reducing the temperature of the growth medium during the process; such that soluble polypeptide may be obtained.
Claim 2
Dependent Claim
58 words
A process for preparing a polypeptide, which process comprises cultivating a host which is capable of expressing said polypeptide in a growth medium for an initial period at a first pH value which favours growth of the host; adjusting the pH to a second value and cultivating the host for a further period at said second pH value.
Claim 3
Dependent Claim
28 words
A process as claimed in claim 2 wherein the process is a fermentation of the fed-batch type and wherein the pH is adjusted before fed-batch conditions are attained.
Claim 4
Dependent Claim
21 words
A process as claimed in claim 2 or 3 wherein the temperature is decreased during the terminal portion of the cultivation.
Claim 5
Dependent Claim
25 words
A process as claimed in claim 4 wherein the temperature is decreased from a first value of about 37°C to a value below about 25°C.
Claim 6
Dependent Claim
28 words
A process as claimed in any one of the preceding claims wherein the pH is adjusted by lowering its value from a first value to a second value.
Claim 7
Dependent Claim
22 words
A process as claimed in claim 6 wherein the host comprises E.coli and the polypeptide comprises ricin A or an analogue thereof.
Claim 8
Dependent Claim
13 words
A process as claimed in claim 7 wherein the host comprises E.coli DS410.
Claim 9
Dependent Claim
29 words
A process as claimed in claim 7 or 8 wherein the pH is adjusted from a value of about 6.7 to a value from about 5.5 to about 6.7.
Claim 10
Dependent Claim
23 words
A process for preparing ricin A which comprises cultivating a host capable of expressing ricin A in which the host comprises E.coli DS410.
Claim 11
Dependent Claim
54 words
A process for preparing a polypeptide, which process comprises cultivating a host capable of expressing said polypeptide in a growth medium at a temperature which favours growth of the host and generation of soluble polypeptide, cooling the growth medium during the terminal portion of the cultivation, and harvesting the host during said terminal portion.
Claim 12
Dependent Claim
13 words
A process as claimed in claim 11 wherein the polypeptide comprises ricin A.
Claim 13
Dependent Claim
44 words
A process for preparing ricin A, or an analogue thereof, said process comprising cultivating a host capable of expressing ricin A, or an analogue thereof, in a growth medium and adding a supplement which includes yeast extract to the growth medium during the cultivation.
Claim 14
Dependent Claim
81 words
A process for preparing ricin A which comprises a fermentation of the fed-batch type in which an E.coli host which is capable of expressing ricin A is cultivated in a growth medium at a pH of about 6.7, the pH is reduced to a value between about 5.5 and 6.7 and cultivated for a further period, and optionally reducing the temperature of the growth medium during the terminal portion of the cultivating and harvesting the ricin A during said terminal portion.
References Cited (Backward Citations)
No citation records indexed.
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