Patent US5962283A successfully refreshed from Google Patents.
US5962283A
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Transminases and amnotransferases
Assignee: BASF Enzymes LLC
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Filing: 1996-05-08
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Granted: 1999-10-05
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Expires: 2016-05-08
Abstract
Abstract Thermostable transaminase and aminotransferase enzymes derived from various ammonifex, aquifex and pyrobaculum organisms are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the pharmaceutical, agricultural and other industries.
Inventors
- 👤 Patrick V. Warren
- 👤 Ronald V. Swanson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:35
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1096; C12N9/14; C12N9/16; C12Y; C12Y206/00; C12Y206/01; C12Y206/01001; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
29 words
An isolated polynucleotide selected from the group consisting of: a) a polynucleotide encoding SEQ ID NOs: 36 or 40; and b) a nucleic acid sequence fully complementary to a).
Claim 2
Dependent Claim
29 words
An isolated polynucleotide selected from the group consisting of: a) a polynucleotide encoding SEQ ID NOs: 36 or 40; and b) a nucleic acid sequence fully complementary to a).
Claim 3
Dependent Claim
10 words
The polynucleotide of claim 1 wherein the polynucleotide is DNA.
Claim 4
Dependent Claim
10 words
The polynucleotide of claim 1 wherein the polynucleotide is RNA.
Claim 5
Dependent Claim
9 words
An expression vector comprising the DNA of claim 2.
Claim 6
Dependent Claim
9 words
A host cell comprising the vector of claim 4.
Claim 7
Dependent Claim
33 words
A process for producing a polypeptide comprising: a) culturing a host cell of claim 5; b) expressing from the host cell a polypeptide encoded by said DNA; and c) optionally isolating the polypeptide.
Claim 8
Dependent Claim
25 words
An enzyme selected from the group consisting of an enzyme comprising the amino acid sequence as set forth in SEQ ID NOS: 36 and 40.
Claim 9
Dependent Claim
25 words
An enzyme selected from the group consisting of an enzyme comprising the amino acid sequence as set forth in SEQ ID NOS: 36 and 40.
Claim 10
Independent Claim
52 words
A method for transferring an amino group from an amino acid to an α-keto acid comprising: contacting an amino acid in the presence of an α-keto acid with an enzyme selected from the group consisting of an enzyme having the amino acid sequence set forth in SEQ ID NOS: 36 and 40.
Claim 11
Independent Claim
52 words
A method for transferring an amino group from an amino acid to an α-keto acid comprising: contacting an amino acid in the presence of an α-keto acid with an enzyme selected from the group consisting of an enzyme having the amino acid sequence set forth in SEQ ID NOS: 36 and 40.
Claim 12
Dependent Claim
20 words
The polynucleotide of claim 2 which encodes an enzyme comprising amino acids 1 to 363 of SEQ ID NO: 36.
Claim 13
Dependent Claim
20 words
The polynucleotide of claim 2 which encodes an enzyme comprising amino acids 1 to 394 of SEQ ID NO: 40.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-10-04 | 2003-04-05 | 2003-10-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-10-04 | 2007-04-05 | 2007-10-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-10-04 | 2011-04-05 | 2011-10-05 | $7700.00 | Window Closed / Lapsed |