US5958199A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor
Assignee: PHC Holdings Corp
•
Filing: 1997-03-12
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Granted: 1999-09-28
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Expires: 2017-03-12
Abstract
Abstract Disclosed is a high performance biosensor free from the effect of any components existing in a sample solution other than a substrate. The biosensor comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on the base plate, and a reaction layer containing a hydrophilic polymer, an enzyme and an electron acceptor located over the electrode system. The reaction layer contains an excess amount of hydrophilic polymer in a range of 150 to 1,000% by weight of the enzyme.
Inventors
- 👤 Yoshiko Miyamoto
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 08:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
110 words
A biosensor comprising: an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on said base plate, and a reaction layer containing a hydrophilic polymer, 0.5 to 50 μg of an enzyme and an electron acceptor formed over said electrode system, wherein said reaction layer contains said hydrophilic polymer in a range of 150 to 1,000% by weight of said enzyme, wherein said reaction layer is dissolved in a sample solution comprising an analyte dissolved in an aqueous media prior to detection of a reaction between said enzyme and said analyte, and wherein said reaction layer is essentially soluble in said sample solution.
Claim 2
Dependent Claim
110 words
A biosensor comprising: an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on said base plate, and a reaction layer containing a hydrophilic polymer, 0.5 to 50 μg of an enzyme and an electron acceptor formed over said electrode system, wherein said reaction layer contains said hydrophilic polymer in a range of 150 to 1,000% by weight of said enzyme, wherein said reaction layer is dissolved in a sample solution comprising an analyte dissolved in an aqueous media prior to detection of a reaction between said enzyme and said analyte, and wherein said reaction layer is essentially soluble in said sample solution.
Claim 3
Dependent Claim
31 words
The biosensor in accordance with claim 1, wherein said hydrophilic polymer is selected from the group consisting of carboxymethyl cellulose, carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxyethylmethyl cellulose, amylose, and starch.
Claim 4
Dependent Claim
44 words
The biosensor in accordance with claim 1, wherein said enzyme is at least one selected from the group consisting of glucose oxidase, glucose dehydrogenase, alcohol oxidase, alcohol dehydrogenase, cholesterol oxidase, cholesterol dehydrogenase, lactate oxidase, lactate dehydrogenase, fructose dehydrogenase, ascorbic acid oxidase, and bilirubin oxidase.
Claim 5
Dependent Claim
33 words
The biosensor in accordance with claim 1, wherein said reaction layer is composed of a first layer containing the hydrophilic polymer and a second layer containing the hydrophilic polymer, enzyme and electron acceptor.
Claim 6
Dependent Claim
37 words
The biosensor in accordance with claim 1, wherein the hydrophilic polymer included in said reaction layer is at least one selected from the group consisting of carboxymethyl cellulose, carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and carboxyethylmethyl cellulose.
Claim 7
Dependent Claim
26 words
The biosensor in accordance with claim 1, wherein an amount of sample solution to be introduced into said biosensor for one measurement is about 3 ul.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-09-27 | 2003-03-29 | 2003-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-09-27 | 2007-03-29 | 2007-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-09-27 | 2011-03-29 | 2011-09-28 | $7700.00 | Window Closed / Lapsed |