Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
84 words
A process for preparation of a biosensor for determining electrochemically a change of substance concentration at the reaction of an enzyme, an electron acceptor and a sample liquid, which comprises arranging an electrode system essentially consisting of at least a working electrode and a counter electrode on an insulating base plate, treating at least the surface of the electrode for measurement with an organic solvent, and arranging a reaction layer consisting of at least an enzyme and an electron acceptor on the electrode system.
Claim 2
Dependent Claim
84 words
A process for preparation of a biosensor for determining electrochemically a change of substance concentration at the reaction of an enzyme, an electron acceptor and a sample liquid, which comprises arranging an electrode system essentially consisting of at least a working electrode and a counter electrode on an insulating base plate, treating at least the surface of the electrode for measurement with an organic solvent, and arranging a reaction layer consisting of at least an enzyme and an electron acceptor on the electrode system.
Claim 3
Dependent Claim
29 words
A process for preparation of a biosensor of the Claim 1, in which the reaction layer consists of at least an oxidoreductase, a hydrophilic polymer and an electron acceptor.
Claim 4
Dependent Claim
35 words
A process for preparation of a biosensor of the Claim 1, in which the electrode system essentially consists of at least a counter electrode and a working electrode made of carbon as a main component.
Claim 5
Dependent Claim
30 words
A process for preparation of a biosensor of the Claim 1, in which the electrode system mainly consisting of carbon is applied on an insulating base plate by screen printing.
Claim 6
Dependent Claim
27 words
A process for preparation of a biosensor of the Claim 1, in which the organic solvent is selected from the group consisting of alcohols, hydrocarbons, and ethers.
Claim 7
Dependent Claim
85 words
A process for preparation of a biosensor of the Claim 2, in which the process comprises forming a first layer containing a hydrophilic polymer and an oxidoreductase on the electrode treated with an organic solvent, forming a second layer of a hydrophilic polymer by applying a solution of a hydrophilic polymer in an organic solvent on the first layer, and then forming a third layer containing an electron acceptor by applying a dispersion of the electron acceptor in an organic solvent on the second layer.
Claim 8
Dependent Claim
40 words
A process for preparation of a biosensor of the Claim 2, in which the reaction layer is formed by applying a solution comprising a hydrophilic polymer, an oxidoreductase, and an electron acceptor on an electrode treated with an organic solvent.
Claim 9
Dependent Claim
52 words
A process for preparation of a biosensor of the Claim 2, in which the reaction layer is formed by forming a layer containing a hydrophilic polymer on an electrode treated with an organic solvent, and applying a solution containing an oxidoreductase and an electron acceptor to said layer containing a hydrophilic polymer.
Claim 10
Dependent Claim
72 words
A process for preparation of a biosensor of the Claim 6, 7 or 8, in which the hydrophilic polymer composing of the reaction layer is selected from the group consisting of cellulose derivatives, gelatine or its derivatives, starch or its derivatives, homo or copolymer containing a residue derived from unsaturated monomer selected from the group consisting of vinyl pyrrolidones, (meth)acrylic acid, salts thereof, maleic anhydride, salts thereof, (meth)acryl amide or salts thereof.