Patent US5942437A successfully refreshed from Google Patents.
US5942437A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and media for enhancing viability maturation, and cryopreservation of cells
Assignee: University of South Florida Research Foundation Inc
•
Filing: 1997-02-11
•
Granted: 1999-08-24
•
Expires: 2017-02-11
Abstract
Abstract A method to increase viability, number, survival and maturation of cells for transplantation or cryopreservation by culturing the cells with Sertoli cells or with sertoli-cell conditioned media (SCM) prior to transplantation (pre-culturing) or cryopreservation.
Inventors
- 👤 Paul R. Sanberg
- 👤 Agneta Othberg
- 👤 Don F. Cameron
- 👤 Samuel Saporta
- 👤 Cesario V. Borlongan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:42
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0681; C12N5/0683; A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; C12N5/0618; C12N5/0619; A61; A61K; A61K35/00; A61K35/12; A61K2035/122; C12N2502/00; C12N2502/24; C12N2502/246
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
46 words
A method of enhancing the viability above control levels of cryopreserved cells, said method comprising the steps of: adding sertoli cell conditioned medium produced by culturing 6×10 5 sertoli cells/cm 2 in a culture medium for 48 hours at 39° C. to cells to be cryopreserved.
Claim 2
Independent Claim
46 words
A method of enhancing the viability above control levels of cryopreserved cells, said method comprising the steps of: adding sertoli cell conditioned medium produced by culturing 6×10 5 sertoli cells/cm 2 in a culture medium for 48 hours at 39° C. to cells to be cryopreserved.
Claim 3
Dependent Claim
37 words
The method as set forth in claim 1, wherein the cells to be cryopreserved include cells of the central nervous system, lymphocytes, hybridomas, fibroblasts, cells for gene therapy, fetal cells, myoblasts, endocrine cells, hepatocytes, or endothelial cells.
Claim 4
Independent Claim
37 words
A method for enhancing the viability of cryopreserved cells, said method comprising the steps of: co-culturing Sertoli cells and cells to be cryopreserved in media and cryopreserving together both the Sertoli cells and cells to be cryopreserved.
Claim 5
Independent Claim
37 words
A method for enhancing the viability of cryopreserved cells, said method comprising the steps of: co-culturing Sertoli cells and cells to be cryopreserved in media and cryopreserving together both the Sertoli cells and cells to be cryopreserved.
Claim 6
Dependent Claim
37 words
A method as set forth in claim 3 wherein said culturing step is further defined as co-culturing the Sertoli cells and cells to be cryopreserved for a period of time ranging from one hour to seven days.
Claim 7
Dependent Claim
39 words
A method of enhancing the viability of cryopreserved cells to be thawed, said method consisting essentially of the step of: adding sertoli cell conditioned medium lacking sertoli cells as set forth in claim 1 to cryopreserved cells upon thawing.
References Cited (Backward Citations)
US5082670A
Cited
WO1995028167A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-08-23 | 2003-02-22 | 2003-08-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-08-23 | 2007-02-22 | 2007-08-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-08-23 | 2011-02-22 | 2011-08-24 | $7700.00 | Window Closed / Lapsed |