US5933233A
Expired / Lapsed
US
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Method and device for the determination of material-specific parameters of one or a few molecules by means of correlation spectroscopy
Assignee: Evotec Biosystems GmbH
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Filing: 1995-10-26
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Granted: 1999-08-03
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Expires: 2015-10-26
Abstract
Abstract A method for the determination of material-specific parameters of one or a few molecules by means of correlation spectroscopy wherein the molecule or molecules in a sample in which the molecule(s) to be determined is (are) present in relatively high concentrations is (are) excited by electromagnetic radiation (excitation radiation) to emit electromagnetic radiation (emission radiation) wherein said excitation and/or emission radiation passes a means which is permeable to the corresponding wavelength of said electromagnetic radiation which means is disposed between an excitation or emission radiation source and an excitation or emission radiation detector, said means for transmitting electromagnetic waves having at least one region the largest dimension of which in at least one direction of space is smaller than the wavelength of said excitation and/or emission radiation of said molecule or molecules.
Inventors
- 👤 Rolf Gunther
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 09:45
CPC Classifications
G; G01; G01Q; G01Q60/00; G01Q60/18; G01Q60/22; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; Y; Y10; Y10S; Y10S977/00; Y10S977/84; Y10S977/88; Y10S977/881; Y10S977/902; Y10S977/932; Y10S977/949; Y10S977/95; Y10S977/953; Y10S977/954
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
138 words
A method for the determination of material-specific parameters of one or a few molecules by means of correlation spectroscopy, characterized in that the molecule or molecules in a sample in which the molecule(s) to be determined is (are) present in relatively high concentrations is (are) excited by electromagnetic radiation (excitation radiation) to emit electromagnetic radiation (emission radiation) wherein said excitation and/or emission radiation passes a means which is permeable to the corresponding wavelength of said electromagnetic radiation which means is disposed in a beam path between an excitation or emission radiation source and an excitation or emission radiation detector, said means for transmitting electromagnetic waves having at least one region the largest dimension of which in at least one direction of space is smaller than the wavelength of said excitation and/or emission radiation of said molecule or molecules.
Claim 2
Independent Claim
138 words
A method for the determination of material-specific parameters of one or a few molecules by means of correlation spectroscopy, characterized in that the molecule or molecules in a sample in which the molecule(s) to be determined is (are) present in relatively high concentrations is (are) excited by electromagnetic radiation (excitation radiation) to emit electromagnetic radiation (emission radiation) wherein said excitation and/or emission radiation passes a means which is permeable to the corresponding wavelength of said electromagnetic radiation which means is disposed in a beam path between an excitation or emission radiation source and an excitation or emission radiation detector, said means for transmitting electromagnetic waves having at least one region the largest dimension of which in at least one direction of space is smaller than the wavelength of said excitation and/or emission radiation of said molecule or molecules.
Claim 3
Dependent Claim
28 words
The method according to claim 1, characterized in that said means which is permeable to electromagnetic radiation has regions in the form of pinhole apertures or slit diaphragms.
Claim 4
Dependent Claim
27 words
The method according to claim 2, characterized in that said regions are optically coupled with focusing optical elements through optical waveguides and/or integrated into said optical waveguides.
Claim 5
Dependent Claim
28 words
The method according to claim 1, wherein the electromagnetic radiation of emission and/or excitation has frequencies within the typical range of luminescence, nuclear resonance fluorescence and X-ray fluorescence.
Claim 6
Dependent Claim
34 words
The method according to claim 1, wherein the interaction of luminescence is measured via coupling with other physical effects, coupling to phonons of the surrounding solid phase, or is combined with atomic force microscopy.
Claim 7
Dependent Claim
39 words
The method according to claim 1, wherein the excitation radiation is supplied by a unit which is a semiconductor laser or a frequency-multiplied semiconductor laser wherein light pulses produced by said unit are optionally amplified by means of waveguides.
Claim 8
Dependent Claim
24 words
The method according to claim 1, wherein the emission radiation is detected by a pin layer connected as a photodiode, avalanche photodiodes or photomultipliers.
Claim 9
Dependent Claim
29 words
The method according to claim 1, wherein the source of the radiation to be detected, the detector for the radiation, optics, and circuit elements are arranged on a substrate.
Claim 10
Dependent Claim
41 words
The method according to claim 1, wherein a number of detectors are arranged in such a way that the molecule serving as the source of the emission radiation is observed, when passing the detectors, in a temporal correlation with this passing.
Claim 11
Dependent Claim
22 words
The method according to claim 1, characterized in that electromaganetic radiation having energies between 284 and 543 eV is used for excitation.
Claim 12
Dependent Claim
24 words
The method according to claim 1, characterized in that the illumination of measuring volumes is effected by using holographic grids or binary optical elements.
Claim 13
Dependent Claim
24 words
The method according to claim 1, characterized in that the emission radiation of at least two measuring volumes is detected in an integrated way.
Claim 14
Dependent Claim
22 words
The method according to claim 3, characterized in that said focusing optical elements are microlenses, gradient index lenses, or binary optical elements.
Claim 15
Dependent Claim
34 words
The method according to claim 5, wherein the other physical effects are nuclear magnetic resonance, generally electric, magnetic or electromagnetic fields (spectral line splitting), frequency doubling (SHG,THG), sum or difference frequency, or vibration effects.
Claim 16
Dependent Claim
12 words
The method according to claim 6, wherein the waveguides are glass fibers.
Claim 17
Dependent Claim
114 words
A device for performing the method according to claim 1 comprising, integrated on a substrate (1), an arrangement of an electromagnetic excitation radiation source (3), a means for receiving a sample (2), a means (4) disposed in the beam path of the excitation and/or emission radiation which is permeable to the corresponding wavelength of this electromagnetic radiation, said means for transmitting electromagnetic waves having at least one region the largest dimension of which in at least one direction of space is smaller than the wavelength of said excitation and/or emission radiation, a detector (5) for electromagnetic radiation, and electronic circuit elements (6, 7), optionally with evaluation units for the analysis of emitted electromagnetic radiation.
Claim 18
Dependent Claim
26 words
The device according to claim 16, wherein said means which is permeable to electromagnetic radiation has regions in the form of pinhole apertures or slit diaphragms.
Claim 19
Independent Claim
34 words
The device according to clam 16, wherein said means which is permeable to electromagnetic radiation has regions which are optically coupled with focusing optical elements through optical waveguides and/or integrated into said optical waveguides.
Claim 20
Independent Claim
34 words
The device according to clam 16, wherein said means which is permeable to electromagnetic radiation has regions which are optically coupled with focusing optical elements through optical waveguides and/or integrated into said optical waveguides.
Claim 21
Dependent Claim
13 words
The device according to claim 16, wherein said substrate is a silicon wafer.
Claim 22
Dependent Claim
44 words
The device according to claim 16, wherein said detector is designed as a photodetector and disposed at the bottom of a channel traversing the substrate and the excitation radiation can in particular be irradiated at an angle of 0°<angle<180° with respect to the detector.
Claim 23
Dependent Claim
16 words
The device according to claim 16, characterized in that said detector is designed as a multidetector.
Claim 24
Dependent Claim
15 words
The device according to claim 16, characterized in that said substrate is a piezoelectric element.
Claim 25
Dependent Claim
21 words
The device according to claim 18, wherein said focusing optical elements are as microlenses, gradient index lenses or binary optical elements.
References Cited (Backward Citations)
US5446534A
Cited
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