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Patent US5916265A successfully refreshed from Google Patents.
US5916265A
Expired / Lapsed
US
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Method of producing a biological extracellular matrix for use as a cell seeding scaffold and implant
Assignee: Individual
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Filing: 1995-06-06
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Granted: 1999-06-29
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Expires: 2015-06-06
Abstract
Abstract Tissue is procured from a human or animal donor. The tissue may be fixed by application of a fixative. Cellular components which would cause rejection are removed from tissue by a chemical treatment that allows the extracellular matrices (ECM) to retain their original shapes, biological structures and ultrastructures, locations and durability. The resulting ECM serves as a cell scaffold on which living cells are seeded to form a lifegraft for implanting or for allograft reconstruction.
Inventors
- 👤 Jie Hu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:55
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3687; A61L27/3604; A61L27/3633; A61L27/38; Y; Y10; Y10S; Y10S128/00; Y10S128/08
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
53 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix, comprising the steps of: a. procuring tissue; b. fixing said tissue by application of a cross linking agent; and c. subsequently decellularizing said tissue by applying a solution comprising a compound having a hydroxyl group to said tissue.
Claim 2
Independent Claim
53 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix, comprising the steps of: a. procuring tissue; b. fixing said tissue by application of a cross linking agent; and c. subsequently decellularizing said tissue by applying a solution comprising a compound having a hydroxyl group to said tissue.
Claim 3
Dependent Claim
32 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix as described in claim 1, further comprising a step of washing the tissue with a buffer solution.
Claim 4
Dependent Claim
46 words
A method of procuring biological implants by removing cellular components and forming an extracellular matrix as described in claim 2, further comprising the steps of incubating said extracellular matrix in an incubation solution, eliminating residual free crosslinking solution and reducing the alkalinity of said extracellular matrix.
Claim 5
Dependent Claim
27 words
A method of procuring biological implants by removing cellular components and forming an extracellular matrix as described in claim 3, wherein said incubation solution is aspartic acid.
Claim 6
Dependent Claim
27 words
A method of procuring biological implants by removing cellular components and forming an extracellular matrix as described in claim 3, wherein said incubation solution is glutamic acid.
Claim 7
Dependent Claim
28 words
A method of procuring biological implants by removing cellular components and forming an extracellular matrix as described in claim 3, wherein said incubation solution is a glycine solution.
Claim 8
Independent Claim
64 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix, comprising the steps of: a. procuring tissue; b. fixing said tissue by application of a cross linking agent; c. subsequently decellularizing said tissue by applying a solution comprising a compound having a hydroxyl group to said tissue; and d. seeding said tissue with living cells to form a graft.
Claim 9
Independent Claim
64 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix, comprising the steps of: a. procuring tissue; b. fixing said tissue by application of a cross linking agent; c. subsequently decellularizing said tissue by applying a solution comprising a compound having a hydroxyl group to said tissue; and d. seeding said tissue with living cells to form a graft.
Claim 10
Dependent Claim
40 words
A method of preparing biological implants by removing cellular components and forming an extracellular matrix as described in claim 7, further comprising a step of washing the tissue with a buffer solution prior to seeding the tissue with living cells.
Claim 11
Dependent Claim
54 words
A method of procuring biological implants by removing cellular components and forming an extracellular matrix as described in claim 8, further comprising the steps of incubating said extracellular matrix in an incubation solution, eliminating residual free crosslinking solution and reducing the alkalinity of said extracellular matrix prior to seeding the tissue with living cells.
Claim 12
Dependent Claim
112 words
A biological extracellular matrix for use in grafting produced by a method comprising the steps of: a. procuring a section of biological tissue; b. preserving the biological components and structures of the section of biological tissue by fixing said section of biological tissue by means of application of a cross linking agent; c. applying an alkali solution comprising a compound having a hydroxyl group to said section of biological tissue; d. applying an additional solution to the section of biological tissue to remove residual cross linking agent from the tissue; and e. washing the section of biological tissue with a buffer solution to neutralize the pH of said section of biological tissue.
Claim 13
Dependent Claim
112 words
A biological extracellular matrix for use in grafting produced by a method comprising the steps of: a. procuring a section of biological tissue; b. preserving the biological components and structures of the section of biological tissue by fixing said section of biological tissue by means of application of a cross linking agent; c. applying an alkali solution comprising a compound having a hydroxyl group to said section of biological tissue; d. applying an additional solution to the section of biological tissue to remove residual cross linking agent from the tissue; and e. washing the section of biological tissue with a buffer solution to neutralize the pH of said section of biological tissue.
Claim 14
Dependent Claim
42 words
A biological extracellular matrix for use in grafting produced by the method described in claim 10 further comprising the step of seeding living cells onto said section of biological tissue after said section of biological tissue is washed with said buffer solution.
Claim 15
Dependent Claim
27 words
A biological extracellular matrix produced by the method described in claim 10, further comprising the step of implanting said section of biological tissue as an extracellular matrix.
Claim 16
Dependent Claim
35 words
A biological extracellular matrix produced by the method described in claim 10, wherein said additional solution is changed periodically until the biological extracellular matrix is semi-transparent and the pH of the solution is about 7.4.
Claim 17
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 1, wherein the solution is a sodium hydroxide solution.
Claim 18
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 10, wherein the solution is a sodium hydroxide solution.
Claim 19
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 11, wherein the solution is a sodium hydroxide solution.
Claim 20
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 12, wherein the solution is a sodium hydroxide solution.
Claim 21
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 1, wherein the solution is a potassium hydroxide solution.
Claim 22
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 10, wherein the solution is a potassium hydroxide solution.
Claim 23
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 11, wherein the solution is a potassium hydroxide solution.
Claim 24
Dependent Claim
20 words
A biological extracellular matrix produced by the method described in claim 12, wherein the solution is a potassium hydroxide solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-06-28 | 2002-12-28 | 2003-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-06-28 | 2006-12-28 | 2007-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-06-28 | 2010-12-28 | 2011-06-29 | $7700.00 | Window Closed / Lapsed |