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US5876997A Expired / Lapsed US ⏱️ 0.0 years remaining

Phytase

Assignee: BASF Enzymes LLC
•
Filing: 1997-08-13
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Granted: 1999-03-02
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Expires: 2017-08-13
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Abstract

Abstract The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.

Inventors

  • 👤 Keith Kretz

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-04 06:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/16; A; A23; A23K; A23K20/00; A23K20/10; A23K20/189; A23L; A23L29/00; A23L29/06; A23L5/00; A23L5/20; A23L5/25; A61; A61P; A61P43/00; C12Y; C12Y301/00; C12Y301/03; C12Y301/03026

Claims Analysis

Total Claims: 11
Claim 1
Independent Claim 23 words
An isolated polynucleotide selected from the group consisting of: a) SEQ ID NO:1; b) SEQ ID NO:1 wherein T is substituted with U.
Claim 2
Dependent Claim 23 words
An isolated polynucleotide selected from the group consisting of: a) SEQ ID NO:1; b) SEQ ID NO:1 wherein T is substituted with U.
Claim 3
Dependent Claim 15 words
An isolated polynucleotide encoding a phytase having the amino acid sequence of SEQ ID NO:2.
Claim 4
Dependent Claim 15 words
An isolated polynucleotide encoding a phytase having the amino acid sequence of SEQ ID NO:2.
Claim 5
Dependent Claim 13 words
The polynucleotide of claim 2, wherein the polynucleotide is isolated from a prokaryote.
Claim 6
Dependent Claim 9 words
An expression vector comprising the polynucleotide of claim 2.
Claim 7
Dependent Claim 11 words
The vector of claim 4, wherein the vector is a plasmid.
Claim 8
Dependent Claim 10 words
The vector of claim 4, wherein the vector is virus-derived.
Claim 9
Dependent Claim 10 words
A host cell transformed with the vector of claim 4.
Claim 10
Dependent Claim 11 words
The host cell of claim 7, wherein the cell is prokaryotic.
Claim 11
Dependent Claim 32 words
A method for producing an enzyme comprising growing a host cell of claim 7 under conditions which allow the expression of the enzyme and isolating the enzyme encoded by the nucleic acid.

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2002-03-01 2002-08-31 2003-03-02 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2006-03-01 2006-08-31 2007-03-02 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2010-03-01 2010-08-31 2011-03-02 $7700.00 Window Closed / Lapsed

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