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US5859347A
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Enhanced expression in plants
Assignee: FFEI Ltd
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Filing: 1996-11-25
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Granted: 1999-01-12
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Expires: 2016-11-25
Abstract
Abstract This invention provides HSP70 introns that when present in a non-translated leader of a chimeric gene enhance expression in plants.
Inventors
- 👤 Sherri Marie Brown
- 👤 Colleen Gail Santino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:23
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C07; C07K; C07K14/00; C07K14/415
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
84 words
An isolated DNA segment comprising in sequence: (a) a promotor that functions in monocotyledonous plant cells; (b) a non-translated leader DNA comprising an intron sequence including at least sufficient nucleotide sequence from the 5' end and from the 3' end of SEQ ID NO: 1 to splice said intron sequence; and (c) a DNA sequence that in combination with (a) and (b) effects production of an RNA sequence; wherein the non-translated leader of (b) increases expression of the DNA sequence relative to wild-type expression.
Claim 2
Dependent Claim
84 words
An isolated DNA segment comprising in sequence: (a) a promotor that functions in monocotyledonous plant cells; (b) a non-translated leader DNA comprising an intron sequence including at least sufficient nucleotide sequence from the 5' end and from the 3' end of SEQ ID NO: 1 to splice said intron sequence; and (c) a DNA sequence that in combination with (a) and (b) effects production of an RNA sequence; wherein the non-translated leader of (b) increases expression of the DNA sequence relative to wild-type expression.
Claim 3
Dependent Claim
34 words
The isolated DNA segment of claim 1, further comprising a 3' non-translated sequence that functions in plant cells to cause addition of a polyadenylated nucleotide sequence to the 3' end of said RNA sequence.
Claim 4
Dependent Claim
33 words
The isolated DNA segment of claim 1, wherein said intron sequence comprises at least about 162 nucleotides created by ligating a 5' end sequence and a 3' end sequence from SEQ ID NO:1.
Claim 5
Dependent Claim
15 words
The isolated DNA segment of claim 3, wherein said intron sequence is SEQ ID NO:3.
Claim 6
Dependent Claim
34 words
The isolated DNA segment of claim 3, wherein said intron sequence comprises at least about 283 nucleotides created by ligating a 5' end sequence and a 3' end sequence from SEQ ID NO: 1.
Claim 7
Dependent Claim
15 words
The isolated DNA segment of claim 5, wherein said intron sequence is SEQ ID NO:2.
Claim 8
Dependent Claim
16 words
The isolated DNA segment of claim 5, wherein said intron sequence is SEQ ID NO: 1.
Claim 9
Dependent Claim
30 words
The isolated DNA segment of claim 1, fused to said leader DNA further comprising between about 10 and about 50 additional nucleotides of an exon fused to said leader DNA.
Claim 10
Dependent Claim
25 words
The isolated DNA segment of claim 1, further comprising between about 11 and about 28 additional nucleotides of an exon fused to said leader DNA.
Claim 11
Dependent Claim
47 words
The isolated DNA segment of claim 1, further comprising between about 10 and about 50 additional nucleotides from the 3' end of HSP70 exon 1 and between about 11 and about 28 additional nucleotides from the 5' end of HSP70 exon 2 fused to said leader DNA.
Claim 12
Dependent Claim
17 words
The isolated DNA segment of claim 1, wherein said intron sequence comprises a splice site consensus sequence.
Claim 13
Dependent Claim
18 words
The isolated DNA segment of claim 11, wherein said intron sequence comprises a 5' splice site consensus sequence.
Claim 14
Dependent Claim
18 words
The isolated DNA segment of claim 11, wherein said intron sequence comprises a 3' splice site consensus sequence.
Claim 15
Dependent Claim
25 words
The isolated DNA segment of claim 11, wherein said intron sequence comprises a 5' splice site consensus sequence and a 3' splice site consensus sequence.
Claim 16
Dependent Claim
23 words
The isolated DNA segment of claim 11, wherein said intron sequence has an adenine nucleotide at position 15 of SEQ ID NO: 1.
Claim 17
Dependent Claim
23 words
The isolated DNA segment of claim 11, wherein said intron sequence has an adenine nucleotide at position 20 of SEQ ID NO: 1.
Claim 18
Dependent Claim
23 words
The isolated DNA segment of claim 11, wherein said intron sequence has a guanine nucleotide at position 800 of SEQ ID NO: 1.
Claim 19
Dependent Claim
36 words
The isolated DNA segment of claim 14, wherein said intron sequence has an adenine nucleotide at position 15, an adenine nucleotide at position 20, and a guanine nucleotide at position 800 of SEQ ID NO: 1.
Claim 20
Dependent Claim
16 words
The isolated DNA segment of claim 1, wherein said promoter comprises a plant DNA virus promoter.
Claim 21
Dependent Claim
18 words
The isolated DNA segment of claim 14, wherein said promoter is a CaMV35S promoter or an FMV promoter.
Claim 22
Dependent Claim
36 words
The isolated DNA segment of claim 1, wherein said DNA sequence encodes an EPSP synthase, a CP4 protein, an ACC-deaminase, a B.t. crystal toxin, a glgC16 protein, a plant viral coat protein or a GOX protein.
Claim 23
Independent Claim
92 words
A method for the expression of a gene in a monocotyledonous plant, comprising introducing into the plant cell a DNA segment comprising in sequence: (a) a promotor that functions in monocotyledonous plant cells; (b) a non-translated leader comprising an intron sequence including at least sufficient nucleotide sequence from the 5' end and from the 3' end of SEQ ID NO: 1 to splice said intron; and (c) a DNA sequence comprising said gene; wherein the DNA segment comprising (a), (b) and (c) produces an RNA sequence that effects expression of said gene.
Claim 24
Independent Claim
92 words
A method for the expression of a gene in a monocotyledonous plant, comprising introducing into the plant cell a DNA segment comprising in sequence: (a) a promotor that functions in monocotyledonous plant cells; (b) a non-translated leader comprising an intron sequence including at least sufficient nucleotide sequence from the 5' end and from the 3' end of SEQ ID NO: 1 to splice said intron; and (c) a DNA sequence comprising said gene; wherein the DNA segment comprising (a), (b) and (c) produces an RNA sequence that effects expression of said gene.
Claim 25
Dependent Claim
36 words
The method of claim 22, wherein said DNA further comprises a 3' non-translated sequence that functions in monocotyledonous plant cells to cause addition of a polyadenylated nucleotide sequence to the 3' end of said RNA sequence.
Claim 26
Dependent Claim
32 words
The method of claim 22, wherein said intron sequence comprises at least about 162 nucleotides comprised by ligating a 5' end sequence and a 3' end sequence from SEQ ID NO: 1.
Claim 27
Dependent Claim
23 words
The method of claim 22, further comprising between about 10 and about 50 additional nucleotides of an exon fused to said leader DNA.
Claim 28
Dependent Claim
15 words
The method of claim 22, wherein said intron sequence comprises a splice site consensus sequence.
Claim 29
Dependent Claim
34 words
The method of claim 22, wherein said DNA sequence encodes an EPSP synthase, a CP4 protein, an ACC-deaminase, a B.t. crystal toxin, a glgC16 protein, a plant viral coat protein or a GOX protein.
Claim 30
Dependent Claim
13 words
The method of claim 22, wherein said plant is maize, wheat or rice.
Claim 31
Dependent Claim
10 words
A transgenic monocotyledonous plant comprising the DNA of claim 11.
Claim 32
Dependent Claim
35 words
The plant of claim 29, wherein said DNA further comprises a 3' non-translated sequence that functions in plant cells to cause addition of a polyadenylated nucleotide sequence to the 3' end of said RNA sequence.
Claim 33
Dependent Claim
32 words
The plant of claim 29, wherein said intron sequence comprises at least about 162 nucleotides obtained by ligating a 5° end sequence and a 3° end sequence from SEQ ID NO: 1.
Claim 34
Dependent Claim
23 words
The plant of claim 29, further comprising between about 10 and about 50 additional nucleotides of an exon fused to said leader DNA.
Claim 35
Dependent Claim
15 words
The plant of claim 29, wherein said intron sequence comprises a splice site consensus sequence.
Claim 36
Dependent Claim
34 words
The plant of claim 29, wherein said DNA sequence encodes an EPSP synthase, a CP4 protein, an ACC-deaminase, a B.t. crystal toxin, a glgC16 protein, a plant viral coat protein or a GOX protein.
Claim 37
Dependent Claim
13 words
The plant of claim 29, wherein said plant is maize, wheat or rice.
References Cited (Backward Citations)
US5424412A
Cited
US5593874A
Cited
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