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US5850018A
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Expression control sequence for general and effective expression of genes in plants
Assignee: Pioneer Hi-Bred International Inc
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Filing: 1996-07-26
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Granted: 1998-12-15
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Expires: 2016-07-26
Abstract
Abstract An expression control sequence which is intermediate in tissue specificity between constitutive and tissue specific is disclosed. This promoter is effective in expressing genes in the majority of tissues of corn, and can be used for effective expression of desired protein genes in plants.
Inventors
- 👤 Chris Baszczynski
- 👤 Eric Barbour
- 👤 Jeannine Horowitz
- 👤 Jeffrey L. Rosichan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 12:39
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C07; C07K; C07K14/00; C07K14/415; C12N15/8222; C12N15/8223; C12N15/8225; C12N15/8226; C12N15/8227; C12N15/823; C12N15/8234; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8286; C02; C02F; C02F2303/00; C02F2303/04; Y; Y02; Y02A; Y02A40/00; Y02A40/10; Y02A40/146
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
33 words
A purified and isolated DNA molecule comprising the transcriptional promoter and/or the leader sequence of the nucleotide sequence of the control sequence shown in positions -812 to -1 of SEQ ID NO: 1.
Claim 2
Dependent Claim
33 words
A purified and isolated DNA molecule comprising the transcriptional promoter and/or the leader sequence of the nucleotide sequence of the control sequence shown in positions -812 to -1 of SEQ ID NO: 1.
Claim 3
Dependent Claim
26 words
The DNA molecule of claim 1 which comprises the nucleotide sequence of the control sequence shown in positions -812 to -1 of SEQ ID NO: 1.
Claim 4
Dependent Claim
33 words
A composition of DNA molecules consisting of DNA molecules which contain the transcriptional promoter and/or the leader sequence of the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1.
Claim 5
Dependent Claim
33 words
A composition of DNA molecules consisting of DNA molecules which contain the transcriptional promoter and/or the leader sequence of the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1.
Claim 6
Dependent Claim
24 words
The composition of claim 3 wherein said DNA molecules contain the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1.
Claim 7
Dependent Claim
43 words
A recombinant expression system which comprises the transcriptional promoter and/or the leader sequence of the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1 operably linked to the coding sequence for a desired protein heterologous to said nucleotide sequence.
Claim 8
Dependent Claim
43 words
A recombinant expression system which comprises the transcriptional promoter and/or the leader sequence of the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1 operably linked to the coding sequence for a desired protein heterologous to said nucleotide sequence.
Claim 9
Dependent Claim
37 words
The expression system of claim 5 which comprises the nucleotide sequence shown as positions -812 to -1 in SEQ ID NO: 1 operably linked to the coding sequence for a desired protein heterologous to said nucleotide sequence.
Claim 10
Dependent Claim
18 words
The expression system of claim 5 wherein said desired protein is an insecticidal protein or an antifungal protein.
Claim 11
Dependent Claim
18 words
The expression system of claim 6 wherein said desired protein is an antifungal protein or an insecticidal protein.
Claim 12
Dependent Claim
22 words
A plant, plant part, or plant cell, wherein said plant is a monocot modified to contain the expression system of claim 5.
Claim 13
Dependent Claim
22 words
A plant, plant part, or plant cell, wherein said plant is a monocot modified to contain the expression system of claim 6.
Claim 14
Dependent Claim
36 words
A method to protect monocot plants against insects or fungi which method comprises modifying said plants to contain the expression system of claim 7 and culturing the plants under conditions for expression of said coding sequence.
Claim 15
Dependent Claim
36 words
A method to protect monocot plants against insects or fungi which method comprises modifying said plants to contain the expression system of claim 8 and culturing the plants under conditions for expression of said coding sequence.
Claim 16
Dependent Claim
33 words
A purified and isolated DNA comprising fragments of the control sequence shown in positions -812 to -1 of SEQ ID NO: 1 which retain transcription-initiating activity and/or the function of the leader sequence.
Claim 17
Dependent Claim
33 words
A purified and isolated DNA comprising fragments of the control sequence shown in positions -812 to -1 of SEQ ID NO: 1 which retain transcription-initiating activity and/or the function of the leader sequence.
Claim 18
Dependent Claim
62 words
A method to regulate the expression of a gene under control of the ZMDJl control sequence in plant cell, plant parts or plants, wherein said plant is a monocot, which method comprises modifying said cell, part or plant containing said gene under control of said ZMDJl control sequence to contain the DNA of claim 13 or RNA of the same nucleotide sequence.
Claim 19
Dependent Claim
25 words
The method of claim 14 wherein said RNA is provided by modifying said cell, part or plant to contain an expression system for said RNA.
Claim 20
Dependent Claim
23 words
A plant cell, plant part or plant, wherein said plant is a monocot, modified to contain the DNA or RNA of claim 14.
References Cited (Backward Citations)
US5187267A
Cited
WO1993007278A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-12-14 | 2002-06-15 | 2002-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-12-14 | 2006-06-15 | 2006-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-12-14 | 2010-06-15 | 2010-12-15 | $7700.00 | Window Closed / Lapsed |