Patent US5834203A successfully refreshed from Google Patents.
US5834203A
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Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
Assignee: Applied Spectral Imaging Ltd
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Filing: 1997-08-25
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Granted: 1998-11-10
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Expires: 2017-08-25
Abstract
Abstract A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Inventors
- 👤 Nir Katzir
- 👤 David Wine
- 👤 Yuval Garini
- 👤 Dario Cabib
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:58
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N21/645; G01N21/6456; G01N21/6458; G02; G02B; G02B21/00; G02B21/06; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N2021/6439; G01N2021/6441
Claims Analysis
Total Claims: 55
Claim 1
Independent Claim
153 words
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of: (a) providing a plurality of wide-band emission filters; (b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 2
Independent Claim
153 words
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of: (a) providing a plurality of wide-band emission filters; (b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein at least two of said plurality of wide-band emission filters have overlapping bandpasses.
Claim 4
Dependent Claim
35 words
The method of claim 1, wherein pixels belonging to each of said groups of pixels are given a unique artificial color, such that pixels belonging to each of said groups are distinguishable from one another.
Claim 5
Dependent Claim
13 words
The method of claim 1, wherein said algorithm is a linear decomposition algorithm.
Claim 6
Dependent Claim
36 words
The method of claim 1, wherein said fluorophores are bound to genetic material of metaphase chromosomes, such that genetic material of each of said metaphase chromosomes is bound to a different fluorophore or combination of fluorophores.
Claim 7
Dependent Claim
15 words
The method of claim 5, wherein the number of said plurality of fluorophores is five.
Claim 8
Dependent Claim
17 words
The method of claim 6, wherein the number of said plurality of wideband emission filters is five.
Claim 9
Dependent Claim
23 words
The method of claim 1, wherein the number of said plurality of fluorophores equals the number of said plurality of wide-band emission filters.
Claim 10
Dependent Claim
46 words
The method of claim 1, wherein each of said plurality of wide-band filters is selected to have a bandpass corresponding to said emission spectrum of one fluorophore of said plurality of fluorophores and to thereby allow a high throughput of light emitted from said one fluorophore.
Claim 11
Dependent Claim
30 words
The method of claim 9, wherein said bandpass of at least one of said plurality of wide-band filters is selected to overlap with said emission peak of its corresponding fluorophore.
Claim 12
Dependent Claim
18 words
The method of claim 1, wherein said plurality of wide-band filters are represented by a single tunable filter.
Claim 13
Dependent Claim
23 words
The method of claim 11, wherein said tunable filter is selected from the group consisting of acousto-optic tunable filter and liquid-crystal tunable filter.
Claim 14
Independent Claim
186 words
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of: (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 15
Independent Claim
186 words
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of: (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 16
Dependent Claim
17 words
The method of claim 13, wherein at least two of said wide-band emission filters have overlapping bandpasses.
Claim 17
Dependent Claim
17 words
The method of claim 13, wherein at least two of said wide-band excitation filters have overlapping bandpasses.
Claim 18
Dependent Claim
17 words
The method of claim 14, wherein at least two of said wide-band excitation filters have overlapping bandpasses.
Claim 19
Dependent Claim
35 words
The method of claim 13, wherein pixels belonging to each of said groups of pixels are given a unique artificial color, such that pixels belonging to each of said groups are distinguishable from one another.
Claim 20
Dependent Claim
13 words
The method of claim 13, wherein said algorithm is a linear decomposition algorithm.
Claim 21
Dependent Claim
36 words
The method of claim 13, wherein said fluorophores are bound to genetic material of metaphase chromosomes, such that genetic material of each of said metaphase chromosomes is bound to a different fluorophore or combination of fluorophores.
Claim 22
Dependent Claim
15 words
The method of claim 19, wherein the number of said plurality of fluorophores is five.
Claim 23
Dependent Claim
17 words
The method of claim 20, wherein the number of said plurality of pairs of filters is five.
Claim 24
Dependent Claim
23 words
The method of claim 13, wherein the number of said plurality of fluorophores equals the number of said plurality of pairs of filters.
Claim 25
Dependent Claim
45 words
The method of claim 13, wherein each of said wide-band emission filters is selected to have a bandpass corresponding to said emission spectrum of one fluorophore of said plurality of fluorophores and to thereby allow a high throughput of light emitted from said one fluorophore.
Claim 26
Dependent Claim
41 words
The method of claim 13, wherein each of said wide-band excitation filters is selected to have a bandpass corresponding to said excitation spectrum of one fluorophore of said plurality of fluorophores and to thereby allow a high throughput of excitation light.
Claim 27
Dependent Claim
29 words
The method of claim 23, wherein said bandpass of at least one of said wide-band emission filters is selected to overlap with said emission peak of its corresponding fluorophore.
Claim 28
Dependent Claim
29 words
The method of claim 24, wherein said bandpass of at least one of said wide-band excitation filters is selected to overlap with said excitation peak of its corresponding fluorophore.
Claim 29
Dependent Claim
17 words
The method of claim 13, wherein said wide-band emission filters are represented by a single tunable filter.
Claim 30
Dependent Claim
23 words
The method of claim 27, wherein said tunable filter is selected from the group consisting of acousto-optic tunable filter and liquid-crystal tunable filter.
Claim 31
Dependent Claim
17 words
The method of claim 13, wherein said wide-band excitation filters are represented by a single tunable filter.
Claim 32
Dependent Claim
23 words
The method of claim 29, wherein said tunable filter is selected from the group consisting of acousto-optic tunable filter and liquid-crystal tunable filter.
Claim 33
Independent Claim
215 words
An apparatus for classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising: (a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for: (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of each of the pixels with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light intensity recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 34
Independent Claim
215 words
An apparatus for classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising: (a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for: (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of each of the pixels with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light intensity recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Claim 35
Dependent Claim
17 words
The apparatus of claim 31, wherein at least two of said wide-band emission filters have overlapping bandpasses.
Claim 36
Dependent Claim
17 words
The apparatus of claim 31, wherein at least two of said wide-band excitation filters have overlapping bandpasses.
Claim 37
Dependent Claim
17 words
The apparatus of claim 32, wherein at least two of said wide-band excitation filters have overlapping bandpasses.
Claim 38
Dependent Claim
39 words
The apparatus of claim 31, wherein said computing device serves for giving pixels belonging to each of said groups of pixels a unique artificial color, such that pixels belonging to each of said groups are distinguishable from one another.
Claim 39
Dependent Claim
13 words
The apparatus of claim 31, wherein said algorithm is a linear decomposition algorithm.
Claim 40
Dependent Claim
17 words
The apparatus of claim 31, wherein the number of said plurality of pairs of filters is five.
Claim 41
Dependent Claim
23 words
The apparatus of claim 31, wherein the number of said plurality of fluorophores equals the number of said plurality of pairs of filters.
Claim 42
Dependent Claim
45 words
The apparatus of claim 31, wherein each of said wide-band emission filters is selected to have a bandpass corresponding to said emission spectrum of one fluorophore of said plurality of fluorophores and to thereby allow a high throughput of light emitted from said one fluorophore.
Claim 43
Dependent Claim
41 words
The apparatus of claim 31, wherein each of said wide-band excitation filters is selected to have a bandpass corresponding to said excitation spectrum of one fluorophore of said plurality of fluorophores and to thereby allow a high throughput of excitation light.
Claim 44
Dependent Claim
29 words
The apparatus of claim 39, wherein said bandpass of at least one of said wide-band emission filters is selected to overlap with said emission peak of its corresponding fluorophore.
Claim 45
Dependent Claim
29 words
The apparatus of claim 40, wherein said bandpass of at least one of said wide-band excitation filters is selected to overlap with said excitation peak of its corresponding fluorophore.
Claim 46
Dependent Claim
17 words
The apparatus of claim 31, wherein said wide-band emission filters are represented by a single tunable filter.
Claim 47
Dependent Claim
23 words
The apparatus of claim 43, wherein said tunable filter is selected from the group consisting of acousto-optic tunable filter and liquid-crystal tunable filter.
Claim 48
Dependent Claim
17 words
The apparatus of claim 31, wherein said wide-band excitation filters are represented by a single tunable filter.
Claim 49
Dependent Claim
22 words
The apparatus of claim 45, wherein said tunable filter is selected from the group consisting of acousto-optic tunable and liquid-crystal tunable filter.
Claim 50
Independent Claim
117 words
A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of: (a) providing a plurality of wide-band emission filters; (b) recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
Claim 51
Independent Claim
117 words
A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of: (a) providing a plurality of wide-band emission filters; (b) recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
Claim 52
Independent Claim
154 words
A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of: (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of the pixel with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; and (d) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
Claim 53
Independent Claim
154 words
A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of: (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of the pixel with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; and (d) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
Claim 54
Independent Claim
181 words
An apparatus far determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising: (a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for: (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of the pixel with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
Claim 55
Independent Claim
181 words
An apparatus far determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising: (a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for: (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of the pixel with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
References Cited (Backward Citations)
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