US5814476A
Expired / Lapsed
US
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Process for the production of stochastically-generated transcription or translation products
Assignee: Individual
•
Filing: 1995-06-05
•
Granted: 1998-09-29
•
Expires: 2015-06-05
Abstract
Abstract The present invention is directed to a process for the production of a peptide, polypeptide, or protein having a predetermined property. In accordance with one embodiment, the process begins by producing by way of synthetic polynucleotide coupling, stochastically generated polynucleotide sequences. A library of expression vectors containing such stochastically generated polynucleotide sequences is formed. Next, host cells containing the vectors are cultured so as to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences. Screening or selection is carried out on such host cells to identify a peptide, polypeptide, or protein produced by the host cells which has the predetermined property. The stochastically generated polynucleotide sequence which encodes the identified peptide, polypeptide, or protein is then isolated and used to produce the peptide, polypeptide, or protein having the predetermined property.
Inventors
- 👤 Stuart Alan Kauffman
- 👤 Marc Ballivet
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:56
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1093; C07; C07K; C07K14/00; C07K14/005; C07K14/435; C07K14/475; C07K14/485; C12N15/1048; C12N15/63; C12N15/64; C12N15/66; C12N15/67; C12N15/68; C12N15/70; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2468; C12N9/2471; C12Y; C12Y302/00; C12Y302/01; C12Y302/01023; A; A61; A61K; A61K39/00; C07K2319/00; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 118
Claim 1
Independent Claim
124 words
A process for the production of a transcription product or a translation product, comprising the steps of: producing a stochastically-generated polynucleotide sequence; producing a library of expression vectors comprising said stochastic polynucleotide sequence; transforming or transfecting a competent clone with said library of expression vectors; amplifying said transformed or transfected competent clone; screening and/or selecting said transformed or transfected clone in order to isolate a clone expressing a stochastic polynucleotide sequence capable of synthesizing a transcription product or a translation product having a predetermined property; and isolating said selected or screened transformed clone; isolating a stochastically generated polynucleotide sequence which encodes the identified transcription product or translation product using the isolated sequence to produce the transcription product or translation product having the predetermined property.
Claim 2
Dependent Claim
124 words
A process for the production of a transcription product or a translation product, comprising the steps of: producing a stochastically-generated polynucleotide sequence; producing a library of expression vectors comprising said stochastic polynucleotide sequence; transforming or transfecting a competent clone with said library of expression vectors; amplifying said transformed or transfected competent clone; screening and/or selecting said transformed or transfected clone in order to isolate a clone expressing a stochastic polynucleotide sequence capable of synthesizing a transcription product or a translation product having a predetermined property; and isolating said selected or screened transformed clone; isolating a stochastically generated polynucleotide sequence which encodes the identified transcription product or translation product using the isolated sequence to produce the transcription product or translation product having the predetermined property.
Claim 3
Dependent Claim
138 words
A process for the production of a transcription product or a translation product, comprising the steps of: producing a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into expression vectors to form a diverse population of expression vectors; transforming or transfecting competent clones with said diverse population of expression vectors comprising said stochastic polynucleotide sequences; amplifying said transformed or transfected competent clone; screening and/or selecting said transformed or transfected clones in order to isolate a clone expressing a stochastic polynucleotide capable of synthesizing a transcription product or a translation product having the predetermined property; isolating said selected or screened transformed clone; isolating said stochastic polynucleotide sequence which encodes the identified transcription product or translation product; using the isolated stochastic polynucleotide sequence so as to produce the transcription product or translation product having the predetermined property.
Claim 4
Dependent Claim
138 words
A process for the production of a transcription product or a translation product, comprising the steps of: producing a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into expression vectors to form a diverse population of expression vectors; transforming or transfecting competent clones with said diverse population of expression vectors comprising said stochastic polynucleotide sequences; amplifying said transformed or transfected competent clone; screening and/or selecting said transformed or transfected clones in order to isolate a clone expressing a stochastic polynucleotide capable of synthesizing a transcription product or a translation product having the predetermined property; isolating said selected or screened transformed clone; isolating said stochastic polynucleotide sequence which encodes the identified transcription product or translation product; using the isolated stochastic polynucleotide sequence so as to produce the transcription product or translation product having the predetermined property.
Claim 5
Dependent Claim
105 words
A process for the production of a polynucleotide comprising, producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors; introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells; producing independent clones of the host cells so produced; screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence having at least one desired property; and isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.
Claim 6
Dependent Claim
105 words
A process for the production of a polynucleotide comprising, producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors; introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells; producing independent clones of the host cells so produced; screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence having at least one desired property; and isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.
Claim 7
Dependent Claim
20 words
The process according to claim 3, wherein said stochastic polynucleotide sequence comprises a capacity to specifically bind to a compound.
Claim 8
Dependent Claim
20 words
The process according to claim 4, wherein said compound is selected from the group consisting of peptides, polypeptides and proteins.
Claim 9
Dependent Claim
24 words
The process according to claim 4, wherein said compound further comprises a compound regulating transcriptional activity or a compound regulating replication activity of DNA.
Claim 10
Dependent Claim
21 words
A process according to claim 6, wherein said compound further comprises a regulatory protein controlling the transcription or replication of DNA.
Claim 11
Dependent Claim
109 words
A process for the production of an RNA comprising, producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors; introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells; producing independent clones of transformed or transfected host cells; screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence capable of producing RNA having at least one desired property; and isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.
Claim 12
Dependent Claim
109 words
A process for the production of an RNA comprising, producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences; inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors; introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells; producing independent clones of transformed or transfected host cells; screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence capable of producing RNA having at least one desired property; and isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.
Claim 13
Dependent Claim
19 words
The process according to claim 8, wherein said RNA further comprises a capacity to specifically bind to a compound.
Claim 14
Dependent Claim
18 words
The process according to claim 8, wherein said RNA further comprises the capacity to catalyze a chemical reaction.
Claim 15
Dependent Claim
14 words
The process according to claim 8, wherein said RNA further comprises a transfer RNA.
Claim 16
Dependent Claim
34 words
The process according to claim 1 or 2 wherein said translation product comprises a product having a desired property and is selected from the group consisting of a peptide, a polypeptide or a protein.
Claim 17
Dependent Claim
32 words
The process according to claim 1 or 2 wherein said transcription product comprises a product having a desired property and is selected from the group consisting of a RNA or a DNA.
Claim 18
Dependent Claim
28 words
A library of vectors produced by the process of claim 1 wherein said library comprises stochastic nucleotide sequences encoding for at least 10,000 transcription products or translation products.
Claim 19
Independent Claim
51 words
A method of identifying a polynucleotide having a predetermined property, comprising: (a) producing a population of polynucleotides comprising greater than about 1×10 5 different stochastic polynucleotide sequences; (b) screening said population of polynucleotides for said predetermined property under conditions which allow detection of one or more polynucleotides having said predetermined property.
Claim 20
Independent Claim
51 words
A method of identifying a polynucleotide having a predetermined property, comprising: (a) producing a population of polynucleotides comprising greater than about 1×10 5 different stochastic polynucleotide sequences; (b) screening said population of polynucleotides for said predetermined property under conditions which allow detection of one or more polynucleotides having said predetermined property.
Claim 21
Dependent Claim
20 words
The method of claim 15, wherein step (a) further comprises synthesizing a population of at least partially stochastic polynucleotide sequences.
Claim 22
Dependent Claim
17 words
The method of claim 15 or 16, further comprising amplification of said population of stochastic polynucleotide sequences.
Claim 23
Dependent Claim
25 words
The method of claim 15 or 16, wherein step (a) further comprises expressing said population of stochastic polynucleotide sequences to produce said population of polynucleotides.
Claim 24
Dependent Claim
14 words
The method of claim 15 or 16, wherein said population of polynucleotides comprise DNA.
Claim 25
Dependent Claim
13 words
The method of claim 15 or 16, wherein said population polynucleotides comprise RNA.
Claim 26
Dependent Claim
18 words
The method of claim 15, further comprising isolating said one or more polynucleotide sequences having said predetermined property.
Claim 27
Dependent Claim
14 words
The method of claim 15, wherein said predetermined property comprises binding or chemical catalysis.
Claim 28
Dependent Claim
18 words
The method of claim 22, further comprising improving said predetermined property by in vitro or in vivo mutagenesis.
Claim 29
Dependent Claim
24 words
The method of claim 22, wherein said binding further comprises modification of a biological or chemical property of a compound bound by said polynucleotide.
Claim 30
Dependent Claim
30 words
The method of claim 24, wherein said modification of said biological or chemical property of said compound further comprises stimulating or inhibiting at least one biological function of said compound.
Claim 31
Dependent Claim
20 words
The method of claim 15, wherein said population of polynucleotides comprises greater than about 1×10 5 different stochastic polynucleotide sequences.
Claim 32
Dependent Claim
20 words
The method of claim 15, wherein said population of polynucleotides comprises greater than about 1×10 7 different stochastic polynucleotide sequences.
Claim 33
Dependent Claim
20 words
The method of claim 15, wherein said population of polynucleotides comprises greater than about 1×10 8 different stochastic polynucleotide sequences.
Claim 34
Independent Claim
95 words
A method of identifying a polynucleotide having a binding property to a ligand, comprising: (a) synthesizing a population of stochastic polynucleotide sequences; (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences; (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection one or more polynucleotides having said binding property to said ligand.
Claim 35
Independent Claim
95 words
A method of identifying a polynucleotide having a binding property to a ligand, comprising: (a) synthesizing a population of stochastic polynucleotide sequences; (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences; (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection one or more polynucleotides having said binding property to said ligand.
Claim 36
Dependent Claim
14 words
The method of claim 29, wherein said inserting further comprises hybridization of complementary ends.
Claim 37
Dependent Claim
11 words
The method of claim 29, wherein said inserting further comprises ligation.
Claim 38
Dependent Claim
21 words
The method of claim 29, therein said population of stochastic polynucleotide sequences are produced by stochastic copolymerization of oligonucleotides or polynucleotides.
Claim 39
Dependent Claim
27 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences are produced by copolymerization of nucleotide triphosphates consisting of Adenine, Cytosine, Guanine, Thymine and Uracil.
Claim 40
Dependent Claim
17 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences are produced by chemical synthesis.
Claim 41
Dependent Claim
14 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences comprise DNA.
Claim 42
Dependent Claim
14 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences comprise RNA.
Claim 43
Dependent Claim
11 words
The method of claim 29, wherein the vector is a plasmid.
Claim 44
Dependent Claim
11 words
The method of claim 29, wherein the vector is viral DNA.
Claim 45
Dependent Claim
16 words
The method of claim 29, wherein the vector is a hybrid of plasmid and viral DNA.
Claim 46
Dependent Claim
11 words
The method of claim 29, wherein the vector is a phage.
Claim 47
Dependent Claim
52 words
The method of claim 29, wherein step (c) further comprises digesting the population of vectors with a restriction enzyme having a recognition sequence absent in the vector and reinserting the digested products into said digested population of vectors to form a different population having a greater number of new stochastic polynucleotide sequences.
Claim 48
Dependent Claim
20 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences, comprise at least 1×10 4 different polynucleotide sequences.
Claim 49
Dependent Claim
20 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 5 different polynucleotide sequences.
Claim 50
Dependent Claim
20 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 6 different polynucleotide sequences.
Claim 51
Dependent Claim
20 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 7 different polynucleotide sequences.
Claim 52
Dependent Claim
20 words
The method of claim 29, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 8 different polynucleotide sequences.
Claim 53
Independent Claim
65 words
A method of isolating a polynucleotide having a predetermined property, comprising: (a) producing a population of polynucleotides comprising greater than 1×10 5 different stochastic polynucleotide sequences; (b) screening said population of stochastic polynucleotide sequences for said predetermined property under conditions which allow detection of one or more polynucleotides having said predetermined property, and (c) isolating the one or more polynucleotide sequences having said predetermined property.
Claim 54
Independent Claim
65 words
A method of isolating a polynucleotide having a predetermined property, comprising: (a) producing a population of polynucleotides comprising greater than 1×10 5 different stochastic polynucleotide sequences; (b) screening said population of stochastic polynucleotide sequences for said predetermined property under conditions which allow detection of one or more polynucleotides having said predetermined property, and (c) isolating the one or more polynucleotide sequences having said predetermined property.
Claim 55
Dependent Claim
20 words
The method of claim 47, wherein step (a) further comprises synthesizing a population of at least partially stochastic polynucleotide sequences.
Claim 56
Dependent Claim
17 words
The method of claim 47 or 48, further comprising amplification of said population of stochastic polynucleotide sequences.
Claim 57
Dependent Claim
25 words
The method of claim 47 or 48, wherein step (a) further comprises expressing said population of stochastic polynucleotide sequences to produce said population of polynucleotides.
Claim 58
Dependent Claim
14 words
The method of claim 47 or 48, wherein said population of polynucleotides comprise DNA.
Claim 59
Dependent Claim
14 words
The method of claim 47 or 48, wherein said population of polynucleotides comprise RNA.
Claim 60
Dependent Claim
22 words
The method of claim 47, further comprising determining the sequence of said one or more isolated polynucleotide sequences having said predetermined property.
Claim 61
Dependent Claim
14 words
The method of claim 47, wherein said predetermined property comprises binding or chemical catalysis.
Claim 62
Dependent Claim
18 words
The method of claim 54, further comprising improving said predetermined property by in vitro or in vivo mutagenesis.
Claim 63
Dependent Claim
24 words
The method of claim 54, wherein said binding further comprises modification of a biological or chemical property of a compound bound by said polynucleotide.
Claim 64
Dependent Claim
30 words
The method of claim 56, wherein said modification of said biological or chemical property of said compound further comprises stimulating or inhibiting at least one biological function of said compound.
Claim 65
Dependent Claim
20 words
The method of claim 47, wherein said population of polynucleotides comprises greater than about 1×10 6 different stochastic polynucleotide sequences.
Claim 66
Dependent Claim
20 words
The method of claim 47, wherein said population of polynucleotides comprises greater than about 1×10 7 different stochastic polynucleotide sequences.
Claim 67
Dependent Claim
20 words
The method of claim 47, wherein said population of polynucleotides comprises greater than about 1×10 8 different stochastic polynucleotide sequences.
Claim 68
Independent Claim
108 words
A method of isolating a polynucleotide having a binding property to a ligand, comprising: (a) synthesizing a population of stochastic polynucleotide sequences; (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences; (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection of one or more polynucleotides to said ligand, and (e) isolating the stochastic polynucleotide sequence or sequences having said binding property to said ligand.
Claim 69
Independent Claim
108 words
A method of isolating a polynucleotide having a binding property to a ligand, comprising: (a) synthesizing a population of stochastic polynucleotide sequences; (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences; (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection of one or more polynucleotides to said ligand, and (e) isolating the stochastic polynucleotide sequence or sequences having said binding property to said ligand.
Claim 70
Dependent Claim
14 words
The method of claim 61, wherein said inserting further comprises hybridization of complementary ends.
Claim 71
Dependent Claim
11 words
The method of claim 61, wherein said inserting further comprises ligation.
Claim 72
Dependent Claim
21 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences are produced by stochastic copolymerization of oligonucleotides or polynucleotides.
Claim 73
Dependent Claim
27 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences are produced by copolymerization of nucleotide triphosphates consisting of Adenine, Cytosine, Guanine, Thymine and Uricil.
Claim 74
Dependent Claim
17 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences are produced by chemical synthesis.
Claim 75
Dependent Claim
14 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences comprise DNA.
Claim 76
Dependent Claim
14 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences comprise RNA.
Claim 77
Dependent Claim
11 words
The method of claim 61, wherein the vector is a plasmid.
Claim 78
Dependent Claim
11 words
The method of claim 61, wherein the vector is viral DNA.
Claim 79
Dependent Claim
16 words
The method of claim 61, wherein the vector is a hybrid of plasmid and viral DNA.
Claim 80
Dependent Claim
11 words
The method of claim 61, wherein the vector is a phage.
Claim 81
Dependent Claim
52 words
The method of claim 61, wherein step (c) further comprises digesting the population of vectors with a restriction enzyme having a recognition sequence absent in the vector and reinserting the digested products into said digested population of vectors to form a different population having a greater number of new stochastic polynucleotide sequences.
Claim 82
Dependent Claim
20 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences, comprise at least 1×10 4 different polynucleotide sequences.
Claim 83
Dependent Claim
20 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 5 different polynucleotide sequences.
Claim 84
Dependent Claim
20 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 6 different polynucleotide sequences.
Claim 85
Dependent Claim
20 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 7 different polynucleotide sequences.
Claim 86
Dependent Claim
20 words
The method of claim 61, wherein said population of stochastic polynucleotide sequences, comprise greater than 1×10 8 different polynucleotide sequences.
Claim 87
Independent Claim
34 words
A method of producing a diverse population of polynucleotides, comprising stochastically copolymerizing a population of polynucleotides so as to produce a new population of polynucleotides containing greater than about 1×10 6 different polynucleotide sequences.
Claim 88
Independent Claim
34 words
A method of producing a diverse population of polynucleotides, comprising stochastically copolymerizing a population of polynucleotides so as to produce a new population of polynucleotides containing greater than about 1×10 6 different polynucleotide sequences.
Claim 89
Dependent Claim
21 words
The method of claim 79, wherein said new population of polynucleotides further comprise greater than about 1×10 6 different polynucleotide sequences.
Claim 90
Dependent Claim
21 words
The method of claim 79, which said new population of polynucleotides further comprise greater than about 1×10 7 different polynucleotide sequences.
Claim 91
Dependent Claim
21 words
The method of claim 79, wherein said new population of polynucleotides further comprise greater than about 1×10 8 different polynucleotide sequences.
Claim 92
Dependent Claim
16 words
The method of claim 79, wherein said stochastic copolymerization is effected by hybridization of complementary sequences.
Claim 93
Dependent Claim
13 words
The method of claim 79, wherein said stochastic copolymerization is effected by ligation.
Claim 94
Dependent Claim
12 words
The method of claim 79, wherein said population of polynucleotides comprise DNA.
Claim 95
Dependent Claim
12 words
The method of claim 79, wherein said population of polynucleotides comprise RNA.
Claim 96
Dependent Claim
16 words
The method of claim 79, further comprising introducing said new population of polynucleotides into host cells.
Claim 97
Dependent Claim
15 words
The method of claim 79, wherein said population of polynucleotides further comprise stochastic polynucleotide sequences.
Claim 98
Dependent Claim
18 words
The method of claim 79, wherein said population of polynucleotides further comprise at least partially stochastic polynucleotide sequences.
Claim 99
Dependent Claim
18 words
The method of claim 79, wherein said population of polynucleotides further comprises two or more populations of polynucleotides.
Claim 100
Independent Claim
55 words
A method of producing a diverse population of polynucleotides, comprising: (a) obtaining one or more populations of polynucleotides; (b) cleaving the one or more populations of polynucleotides, and (c) stochastically copolymerizing the one or more populations of cleaved polynucleotides so as to produce a new population of greater than about 1×10 5 different polynucleotide sequences.
Claim 101
Independent Claim
55 words
A method of producing a diverse population of polynucleotides, comprising: (a) obtaining one or more populations of polynucleotides; (b) cleaving the one or more populations of polynucleotides, and (c) stochastically copolymerizing the one or more populations of cleaved polynucleotides so as to produce a new population of greater than about 1×10 5 different polynucleotide sequences.
Claim 102
Dependent Claim
21 words
The method of claim 91, wherein said new population of polynucleotides further comprise greater than about 1×10 6 different polynucleotide sequences.
Claim 103
Dependent Claim
21 words
The method of claim 91, which said new population of polynucleotides further comprise greater than about 1×10 7 different polynucleotide sequences.
Claim 104
Dependent Claim
21 words
The method of claim 91, wherein said new population of polynucleotides further comprise greater than about 1×10 8 different polynucleotide sequences.
Claim 105
Dependent Claim
16 words
The method of claim 91, wherein said stochastic copolymerization is effected by hybridization of complementary sequences.
Claim 106
Dependent Claim
13 words
The method of claim 91, wherein said stochastic copolymerization is effected by ligation.
Claim 107
Dependent Claim
15 words
The method of claim 91, wherein said one or more populations of polynucleotides comprise DNA.
Claim 108
Dependent Claim
15 words
The method of claim 91, wherein said one or more populations of polynuclectides comprise RNA.
Claim 109
Dependent Claim
16 words
The method of claim 91, further comprising introducing said new population of polynucleotides into host cells.
Claim 110
Dependent Claim
15 words
The method of claim 91, wherein said population of polynucleotides further comprise stochastic polynucleotide sequences.
Claim 111
Dependent Claim
18 words
The method of claim 91, wherein said population of polynucleotides further comprise at least partially stochastic polynucleotide sequences.
Claim 112
Dependent Claim
18 words
The method of claim 91, wherein said population of polynucleotides further comprises two or more populations of polynucleotides.
Claim 113
Dependent Claim
15 words
An isolated population of polynucleotides, comprising greater than about 1×10 5 different stochastic polynucleotide sequences.
Claim 114
Dependent Claim
15 words
An isolated population of polynucleotides, comprising greater than about 1×10 5 different stochastic polynucleotide sequences.
Claim 115
Dependent Claim
17 words
The isolated population of claim 103, further comprising greater than about 1×10 6 different stochastic polynucleotide sequences.
Claim 116
Dependent Claim
17 words
The isolated population of claim 103, further comprising greater than about 1×10 7 different stochastic polynucleotide sequences.
Claim 117
Dependent Claim
17 words
The isolated population of claim 103, further comprising greater than about 1×10 8 different stochastic polynucleotide sequences.
Claim 118
Dependent Claim
19 words
The isolated population of claims 103, 104, 105 or 106, wherein said stochastic polynucleotide sequences comprise DNA or RNA.
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