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US4490358A
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Screening vaccines and immunization process
Assignee: Harvard University
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Filing: 1982-03-01
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Granted: 1984-12-25
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Expires: 2002-03-01
Abstract
Abstract Mammals are vaccinated against infectious organisms and polypeptides are screened for utility as vaccines by a complementing set of monoclonal antibodies, the first of which antibodies binds specifically to the site on the organism which itself binds specifically to a receptor on a host cell of the mammal, and the second of which binds specifically to the first. Vaccination is done with the second antibody alone, and screening is done by determining whether the polypeptide binds to the first antibody.
Inventors
- 👤 Mark I. Greene
- 👤 Bernard N. Fields
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:08
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/08; C07K16/10; C07K16/42; C07K16/4208; C07K16/4216; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
77 words
The method of immunizing a host mammal against an infectious organism having a site which binds specifically to a receptor site on a host cell which method comprises providing a first monoclonal antibody which binds specifically to said organism site, providing a second monoclonal antibody which binds specifically to the first, and inoculating said mammal with an effective amount of said second antibody to bind specifically to said receptor site and block binding of said organism thereto.
Claim 2
Independent Claim
77 words
The method of immunizing a host mammal against an infectious organism having a site which binds specifically to a receptor site on a host cell which method comprises providing a first monoclonal antibody which binds specifically to said organism site, providing a second monoclonal antibody which binds specifically to the first, and inoculating said mammal with an effective amount of said second antibody to bind specifically to said receptor site and block binding of said organism thereto.
Claim 3
Dependent Claim
15 words
The method as claimed in claim 1 in which the infectious organism is a virus.
Claim 4
Independent Claim
50 words
The method of screening polypeptides for potential vaccine activity against an infectious organism having a site which binds specifically to a receptor site on a host cell, which method comprises providing a first monoclonal antibody binding specifically to said organism site, and determining whether said polypeptide binds to said antibody.
Claim 5
Independent Claim
50 words
The method of screening polypeptides for potential vaccine activity against an infectious organism having a site which binds specifically to a receptor site on a host cell, which method comprises providing a first monoclonal antibody binding specifically to said organism site, and determining whether said polypeptide binds to said antibody.
Claim 6
Dependent Claim
14 words
The method as claimed in claim 3 in which said organism is a virus.
Claim 7
Independent Claim
33 words
The method as claimed in claims 3 or 4 in which the binding is determined by measuring competitive binding of said polypeptide and of a labelled specimen of said organism to said antibody.
Claim 8
Dependent Claim
40 words
The method of claim 4 in which the binding is determined by measuring competitive binding to said antibody of said polypeptide and of radiolabelled viral hemagglutinin containing a site which binds specifically to a receptor site on a host cell.
Claim 9
Independent Claim
67 words
The method of screening polypeptides for potential vaccine activity against an infectious organism having a site which binds specifically to a receptor site on a host cell, which method comprises providing a first monoclonal antibody binding specifically to said organism site, providing a second antibody binding specifically to the first, and determining whether said polypeptide interferes with the binding of said second antibody to said first antibody.
Claim 10
Independent Claim
67 words
The method of screening polypeptides for potential vaccine activity against an infectious organism having a site which binds specifically to a receptor site on a host cell, which method comprises providing a first monoclonal antibody binding specifically to said organism site, providing a second antibody binding specifically to the first, and determining whether said polypeptide interferes with the binding of said second antibody to said first antibody.
Claim 11
Dependent Claim
14 words
The method as claimed in claim 7 in which said organism is a virus.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-25 | 1988-06-25 | 1988-12-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-25 | 1992-06-25 | 1992-12-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-25 | 1996-06-25 | 1996-12-25 | $7700.00 | Window Closed / Lapsed |