US5807522A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for fabricating microarrays of biological samples
Assignee: Leland Stanford Junior University
•
Filing: 1995-06-07
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Granted: 1998-09-15
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Expires: 2015-06-07
Abstract
Abstract A method and apparatus for forming microarrays of biological samples on a support are disclosed. The method involves dispensing a known volume of a reagent at each selected array position, by tapping a capillary dispenser on the support under conditions effective to draw a defined volume of liquid onto the support. The apparatus is designed to produce a microarray of such regions in an automated fashion.
Inventors
- 👤 Patrick O. Brown
- 👤 Tidhar Dari Shalon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:28
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/02; B01L3/0241; B01L3/0244; B01J; B01J19/00; B01J19/0046; B01J4/00; B01J4/02; B01L3/0248; B01L3/0268; B01L3/50; B01L3/508; B01L3/5088; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6809; C12Q1/6813; C12Q1/6834; C12Q1/6837; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00317; B01J2219/00351; B01J2219/00364; B01J2219/00367; B01J2219/00378; B01J2219/00457; B01J2219/00475; B01J2219/00497; B01J2219/00527; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00619; B01J2219/00621; B01J2219/00623; B01J2219/00626; B01J2219/0063; B01J2219/00632; B01J2219/00637; B01J2219/00639; B01J2219/00641; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; G01N1/00; G01N1/28; G01N1/2813; G01N2001/282; G01N35/00; G01N2035/00178; G01N2035/00237; G01N35/10; G01N2035/1027; G01N2035/1034; G01N2035/1037; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/115831; Y10T436/116664
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
130 words
A method of forming a microarray of discrete analyte-assay regions on a solid support, where each discrete region in the microarray has a selected, analyte-specific reagent, said method comprising, (a) loading an aqueous solution of a selected analyte-specific reagent in a reagent-dispensing device having an elongate capillary channel adapted to hold a quantity of the reagent solution and having a tip region at which the solution in the channel forms a meniscus, (b) tapping the tip of the dispensing device against a solid support at a defined position on the surface, with an impulse effective to break the meniscus in the capillary channel and deposit a selected volume between 0.002 and 2 nl of solution on the surface, and (c) repeating steps (a) and (b) until said microarray is formed.
Claim 2
Independent Claim
130 words
A method of forming a microarray of discrete analyte-assay regions on a solid support, where each discrete region in the microarray has a selected, analyte-specific reagent, said method comprising, (a) loading an aqueous solution of a selected analyte-specific reagent in a reagent-dispensing device having an elongate capillary channel adapted to hold a quantity of the reagent solution and having a tip region at which the solution in the channel forms a meniscus, (b) tapping the tip of the dispensing device against a solid support at a defined position on the surface, with an impulse effective to break the meniscus in the capillary channel and deposit a selected volume between 0.002 and 2 nl of solution on the surface, and (c) repeating steps (a) and (b) until said microarray is formed.
Claim 3
Dependent Claim
51 words
The method of claim 1, wherein the reagents used to form the discrete regions in the microarray are distinct nucleic acid strands and wherein steps (a) and (b) are repeated until the microarray has about 100 or more discrete regions of distinct nucleic acid strands per cm 2 of solid support.
Claim 4
Dependent Claim
51 words
The method of claim 1, wherein the reagents used to form the discrete regions in the microarray are distinct nucleic acid strands and wherein steps (a) and (b) are repeated until the microarray has about 1000 or more discrete regions of distinct nucleic acid strands per cm 2 of solid support.
Claim 5
Dependent Claim
10 words
The method of claim 2, wherein the channel is open-sided.
Claim 6
Dependent Claim
10 words
The method of claim 3, wherein the channel is open-sided.
Claim 7
Dependent Claim
14 words
The method of claim 4, wherein the volume is between 0.002 and 0.25 nl.
Claim 8
Dependent Claim
14 words
The method of claim 5, wherein the volume is between 0.002 and 0.25 nl.
References Cited (Backward Citations)
US3730844A
Cited
US4071315A
Cited
US5328824A
Cited
US4486539A
Cited
US4563419A
Cited
US4556643A
Cited
US4591570A
Cited
US4677054A
Cited
US4731325A
Cited
US4716106A
Cited
US4670380A
Cited
US4755458A
Cited
US5064754A
Cited
US4767700A
Cited
US4683202B1
Cited
US4683202A
Cited
US4868104A
Cited
US4868105A
Cited
US4683195B1
Cited
US4683195A
Cited
US5348855A
Cited
US4981783A
Cited
US5202231A
Cited
US5100777A
Cited
US5028545A
Cited
US4921805A
Cited
US5013669A
Cited
US5204268A
Cited
US5185243A
Cited
US5516641A
Cited
WO1990003382A1
Cited
US5389512A
Cited
US5200051A
Cited
US5200312A
Cited
US5143854A
Cited
US5510270A
Cited
US5445934A
Cited
US5252743A
Cited
US5188963A
Cited
US5091652A
Cited
US5514785A
Cited
US5252296A
Cited
US5338688A
Cited
US5474895A
Cited
WO1992010588A1
Cited
US5556748A
Cited
US5474796A
Cited
US5563060A
Cited
US5242974A
Cited
US5434049A
Cited
US5514543A
Cited
WO1993022680A1
Cited
US5412087A
Cited
US5518883A
Cited
WO1995000530A1
Cited
US5512430A
Cited
US5472842A
Cited
US5605662A
Cited
WO1995015970A1
Cited
WO1995021944A1
Cited
WO1995025116A1
Cited
US5578832A
Cited
EP0721016A2
Cited
US5556752A
Cited
WO1996017958A1
Cited
US5545531A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-09-14 | 2002-03-16 | 2002-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-09-14 | 2006-03-16 | 2006-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-09-14 | 2010-03-16 | 2010-09-15 | $7700.00 | Window Closed / Lapsed |