US5512430A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic array for virus infection
Assignee: Cerus Corp
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Filing: 1993-07-13
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Granted: 1996-04-30
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Expires: 2013-07-13
Abstract
Abstract The use of nucleic acid to diagnose disease is disclosed. The use of nucleic acid from at least one hair follicle to diagnose the presence of a human immunodeficiency virus infection is demonstrated.
Inventors
- 👤 Yu Gong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806; C07; C07K; C07K14/00; C07K14/005; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16022; Y; Y10; Y10S; Y10S435/00; Y10S435/974
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
71 words
A method for detecting a viral nucleic acid sequence from the human immunodeficiency virus in a sample of hair comprising the steps of: a) providing cells from at least one hair follicle, said cells suspected of containing a viral nucleic acid sequence from the human immunodeficiency virus; b) amplifying said viral nucleic acid sequence from the human immunodeficiency virus in said hair follicle; and c) detecting said viral nucleic acid sequence.
Claim 2
Independent Claim
71 words
A method for detecting a viral nucleic acid sequence from the human immunodeficiency virus in a sample of hair comprising the steps of: a) providing cells from at least one hair follicle, said cells suspected of containing a viral nucleic acid sequence from the human immunodeficiency virus; b) amplifying said viral nucleic acid sequence from the human immunodeficiency virus in said hair follicle; and c) detecting said viral nucleic acid sequence.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said vital nucleic acid sequence is DNA.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said vital nucleic acid sequence is RNA.
Claim 5
Independent Claim
61 words
A method for detecting a human immunodeficiency virus infection comprising the steps of: a) providing cells from at least one hair follicle suspected of containing an HIV nucleic acid sequence: b) lysing said cells to make a lysate containing said nucleic acid; c) treating said lysate to amplify said HIV nucleic acid sequence; and d) detecting said HIV nucleic acid sequence.
Claim 6
Independent Claim
61 words
A method for detecting a human immunodeficiency virus infection comprising the steps of: a) providing cells from at least one hair follicle suspected of containing an HIV nucleic acid sequence: b) lysing said cells to make a lysate containing said nucleic acid; c) treating said lysate to amplify said HIV nucleic acid sequence; and d) detecting said HIV nucleic acid sequence.
Claim 7
Dependent Claim
24 words
A method for detecting a human immunodeficiency virus infection as in claim 4 wherein said lysing in step (b) comprises adding a lysing agent.
Claim 8
Dependent Claim
20 words
A method for detecting a human immunodeficiency virus infection as in claim 5 wherein said lysing agent comprises a protease.
Claim 9
Dependent Claim
21 words
A method for detecting a human immunodeficiency virus infection as in claim 6 further comprising the step of inactivating said protease.
Claim 10
Dependent Claim
81 words
A method for detecting a human immunodeficiency virus infection as in claim 4 wherein step (c) comprises: a) providing in any order: i) all standard reagents necessary to amplify at least a portion of said human immunodeficiency virus nucleic acid sequence, and ii) at least one amplification enzyme capable of initiating an amplification reaction resulting in a nucleic acid product, b) adding said reagents so as to form a reaction mixture, and c) adding said amplification enzyme to said reaction mixture.
Claim 11
Dependent Claim
13 words
The method of claim 8 wherein said amplification enzyme is Thermus aquaticus polymerase.
Claim 12
Independent Claim
85 words
A method for detecting a viral nucleic acid sequence from the human immunodeficiency virus in a sample of hair comprising the steps of: a) providing cells from at least one hair follicle suspected of containing a viral nucleic acid sequence from the human immunodeficiency virus; b) lysing said cells with a protease to make a lysate containing said viral nucleic acid sequence: c) inactivating said protease: d) treating said lysate to amplify said viral nucleic acid sequence; and e) detecting said viral nucleic acid sequence.
Claim 13
Independent Claim
85 words
A method for detecting a viral nucleic acid sequence from the human immunodeficiency virus in a sample of hair comprising the steps of: a) providing cells from at least one hair follicle suspected of containing a viral nucleic acid sequence from the human immunodeficiency virus; b) lysing said cells with a protease to make a lysate containing said viral nucleic acid sequence: c) inactivating said protease: d) treating said lysate to amplify said viral nucleic acid sequence; and e) detecting said viral nucleic acid sequence.
Claim 14
Dependent Claim
13 words
The method of claim 10 wherein said viral nucleic acid sequence is DNA.
Claim 15
Dependent Claim
13 words
The method of claim 10 wherein said viral nucleic acid sequence is RNA.
Claim 16
Dependent Claim
71 words
The method of claim 10 wherein step (d) comprises: a) providing in any order: i) all standard reagents necessary to amplify at least a portion of said viral nucleic acid sequence, and ii) at least one amplification enzyme capable of initiating an amplification reaction resulting in a nucleic acid product; b) adding said reagents so as to form a reaction mixture; and c) adding said amplification enzyme to said reaction mixture.
Claim 17
Dependent Claim
13 words
The method of claim 13 wherein said amplification enzyme is Thermus aquaticus polymerase.
References Cited (Backward Citations)
US4683202A
Cited
US4683202B1
Cited
US4683195A
Cited
US4683195B1
Cited
US5310893A
Cited
US4889818A
Cited
US5196305A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-04-30 | 1999-10-30 | 2000-04-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-04-30 | 2003-10-30 | 2004-04-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-04-30 | 2007-10-30 | 2008-04-30 | $7700.00 | Window Closed / Lapsed |