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US5801026A
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Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants
Assignee: Carnegie Institution for Science
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Filing: 1994-10-11
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Granted: 1998-09-01
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Expires: 2014-10-11
Abstract
Abstract The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.
Inventors
- 👤 Chris Somerville
- 👤 Frank van de Loo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:21
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8247; C12N9/0073
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
113 words
A method of increasing the amount of a hydroxylated fatty acyl compound in a seed of a plant from a given percentage of hydroxylated fatty acyl compound to an increased percentage of hydroxylated fatty acyl compound comprising: growing a plant producing said seed having integrated in its genome a DNA construct, said DNA construct comprising in the 5' to 3' direction of transcription, a transcriptional regulatory region functional in said plant and a plant oleate-12 hydroxylase region encoding amino acid sequence SEQ ID NO:40, under conditions which will permit the transcription and translation of said plant oleate-12 hydroxylase region, whereby said increased percentage of hydroxylated fatty acyl compound is produced in said seed.
Claim 2
Independent Claim
113 words
A method of increasing the amount of a hydroxylated fatty acyl compound in a seed of a plant from a given percentage of hydroxylated fatty acyl compound to an increased percentage of hydroxylated fatty acyl compound comprising: growing a plant producing said seed having integrated in its genome a DNA construct, said DNA construct comprising in the 5' to 3' direction of transcription, a transcriptional regulatory region functional in said plant and a plant oleate-12 hydroxylase region encoding amino acid sequence SEQ ID NO:40, under conditions which will permit the transcription and translation of said plant oleate-12 hydroxylase region, whereby said increased percentage of hydroxylated fatty acyl compound is produced in said seed.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein said plant oleate-12 hydroxylase region comprises base 187 to base 1347 of SEQ ID NO:39.
Claim 4
Dependent Claim
29 words
The method of claim 1 wherein said plant is selected from the group consisting of rapeseed, Canola, flax, sunflower, safflower, cotton, Cuphea, soybean, peanut, coconut, oil palm, and corn.
Claim 5
Dependent Claim
31 words
The method of claim 1 wherein said hydroxylated fatty acyl compound comprises a hydroxylated fatty acid selected from the group consisting of 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and 14-hydroxy-11-eicosenoic acid (lesquarolic acid).
Claim 6
Dependent Claim
16 words
The method of claim 1 further comprising metabolizing said hydroxylated fatty acyl compound with an enzyme.
Claim 7
Dependent Claim
22 words
The method of claim 5 wherein said hydroxylated fatty acyl compound comprises 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and said enzyme is a desaturase.
Claim 8
Independent Claim
86 words
A method of producing a hydroxylated fatty acyl compound in a seed of a plant comprising: (a) providing a fatty acyl compound in said seed of said plant, wherein said fatty acyl compound is a substrate for oleate-12 hydroxylase; and (b) expressing oleate-12 hydroxylase from an integrated DNA construct in said seed of said plant to hydroxylate said fatty acyl compound, wherein said DNA construct encodes amino acid sequence SEQ ID NO:40, whereby said hydroxylated fatty acyl compound is produced in said seed of said plant.
Claim 9
Independent Claim
86 words
A method of producing a hydroxylated fatty acyl compound in a seed of a plant comprising: (a) providing a fatty acyl compound in said seed of said plant, wherein said fatty acyl compound is a substrate for oleate-12 hydroxylase; and (b) expressing oleate-12 hydroxylase from an integrated DNA construct in said seed of said plant to hydroxylate said fatty acyl compound, wherein said DNA construct encodes amino acid sequence SEQ ID NO:40, whereby said hydroxylated fatty acyl compound is produced in said seed of said plant.
Claim 10
Dependent Claim
19 words
The method of claim 7 wherein said DNA construct comprises base 187 to base 1347 of SEQ ID NO:39.
Claim 11
Dependent Claim
29 words
The method of claim 7 wherein said plant is selected from the group consisting of rapeseed, Canola, flax, sunflower, safflower, cotton, Cuphea, soybean, peanut, coconut, oil palm, and corn.
Claim 12
Dependent Claim
27 words
The method of claim 7 wherein said fatty acyl compound comprises a fatty acid selected from the group consisting of 9-octadecenoic acid (oleic acid) and 11-eicosenoic acid.
Claim 13
Dependent Claim
31 words
The method of claim 7 wherein said hydroxylated fatty acyl compound comprises a hydroxylated fatty acid selected from the group consisting of 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and 14-hydroxy-11-eicosenoic acid (lesquerolic acid).
Claim 14
Dependent Claim
15 words
The method of claim 7 wherein said fatty acyl compound is provided by transgenic means.
Claim 15
Dependent Claim
16 words
The method of claim 7 further comprising metabolizing said hydroxylated fatty acyl compound with an enzyme.
Claim 16
Dependent Claim
22 words
The method of claim 13 wherein said hydroxylated fatty acyl compound comprises 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and said enzyme is a desaturase.
Claim 17
Independent Claim
129 words
A method of producing a hydroxylated fatty acyl compound in a seed of a plant comprising: (a) providing a fatty acyl compound in said seed of said plant, wherein said fatty acyl compound comprises a fatty acid selected from the group consisting of 9-hexadecenoic acid, 6-octadecenoic acid, 9-octadecenoic acid, 11-eicosenoic acid, and 13-docosenoic acid, wherein said fatty acid contains a double bond; (b) expressing oleate-12 hydroxylase from an integrated DNA construct in said seed of said plant, wherein said DNA construct encodes amino acid sequence SEQ ID NO:40; and (c) hydroxylating said fatty acyl compound with said oleate-12 hydroxylase to produce said hydroxylated fatty acyl compound in said seed of said plant, whereby a hydroxyl group is added three carbons distal of said double bond of said fatty acid.
Claim 18
Independent Claim
129 words
A method of producing a hydroxylated fatty acyl compound in a seed of a plant comprising: (a) providing a fatty acyl compound in said seed of said plant, wherein said fatty acyl compound comprises a fatty acid selected from the group consisting of 9-hexadecenoic acid, 6-octadecenoic acid, 9-octadecenoic acid, 11-eicosenoic acid, and 13-docosenoic acid, wherein said fatty acid contains a double bond; (b) expressing oleate-12 hydroxylase from an integrated DNA construct in said seed of said plant, wherein said DNA construct encodes amino acid sequence SEQ ID NO:40; and (c) hydroxylating said fatty acyl compound with said oleate-12 hydroxylase to produce said hydroxylated fatty acyl compound in said seed of said plant, whereby a hydroxyl group is added three carbons distal of said double bond of said fatty acid.
Claim 19
Dependent Claim
19 words
The method of claim 15 wherein said DNA construct comprises base 187 to base 1347 of SEQ ID NO:39.
Claim 20
Dependent Claim
29 words
The method of claim 15 wherein said plant is selected from the group consisting of rapeseed, Canola, flax, sunflower, safflower, cotton, Cuphea, soybean, peanut, coconut, oil palm, and corn.
Claim 21
Dependent Claim
23 words
The method of claim 15 wherein said fatty acid is selected from the group consisting of 9-octadecenoic acid (oleic acid) and 11-eicosenoic acid.
Claim 22
Dependent Claim
31 words
The method of claim 15 wherein said hydroxylated fatty acyl compound comprises a hydroxylated fatty acid selected from the group consisting of 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and 14-hydroxy-11-eicosenoic acid (lesquerolic acid).
Claim 23
Dependent Claim
15 words
The method of claim 15 wherein said fatty acyl compound is provided by transgenic means.
Claim 24
Dependent Claim
16 words
The method of claim 15 further comprising metabolizing said hydroxylated fatty acyl compound with an enzyme.
Claim 25
Dependent Claim
22 words
The method of claim 21 wherein said hydroxylated fatty acyl compound comprises 12-hydroxy-9-octadecenoic acid (ricinoleic acid) and said enzyme is a desaturase.
References Cited (Backward Citations)
WO1994011516A1
Cited
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