US5798083A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection
Assignee: Bioveris Corp
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Filing: 1995-06-06
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Granted: 1998-08-25
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Expires: 2015-06-06
Abstract
Abstract What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
Inventors
- 👤 Richard J. Massey
- 👤 Gary F. Blackburn
- 👤 Elizabeth W. Wilkins
- 👤 Haresh P. Shah
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:48
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/66; G01N21/69; C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; G01N21/75; G01N21/76; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/54366; G01N33/54373; G01N33/5438; G01N33/58; G01N33/582; G01N33/585; G01N1/00; G01N1/28; G01N1/40; G01N2458/00; G01N2458/30
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
103 words
An apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising: (a) a cell consisting essentially of a sample volume having a vertical columnar zones, inlet means and outlet means for generating a magnetic field positioned proximate to a single collection zone, said collection zone being below a substantial volume of said cell and said columnar zone, and said field being sufficient to collect said analyte of interest at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (b) means to measure the chemiluminescence generated at the collection zone.
Claim 2
Independent Claim
103 words
An apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising: (a) a cell consisting essentially of a sample volume having a vertical columnar zones, inlet means and outlet means for generating a magnetic field positioned proximate to a single collection zone, said collection zone being below a substantial volume of said cell and said columnar zone, and said field being sufficient to collect said analyte of interest at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (b) means to measure the chemiluminescence generated at the collection zone.
Claim 3
Dependent Claim
18 words
An apparatus as recited in claim 1, wherein said means to measure the chemiluminescence is a photomultiplier tube.
Claim 4
Dependent Claim
26 words
An apparatus as recited in claim 1, further comprising a means for reflecting chemiluminescence generated at the collection zone onto said means to measure the chemiluminescence.
Claim 5
Independent Claim
19 words
An apparatus as recited in claims 1, wherein said means for generating said magnetic field is a permanent magnet.
Claim 6
Dependent Claim
18 words
An apparatus as recited in claim 1, wherein said means for generating said magnetic field is an electromagnet.
Claim 7
Dependent Claim
28 words
An apparatus as recited in claim 1, further comprising a housing enclosing said cell and shielding said means to measure the chemiluminescence from external light during chemiluminescent measurements.
Claim 8
Dependent Claim
23 words
An apparatus as recited in claim 1, further comprising a shutter mechanism provided between said cell and said means to measure the chemiluminescence.
Claim 9
Dependent Claim
39 words
An apparatus as recited in claim 7, wherein said shutter mechanism is controllably operated to open only so far as to expose the means to measure the chemiluminescence from the collection zone in the cell during chemiluminescence measurement periods.
Claim 10
Dependent Claim
22 words
An apparatus as recited in claim 1, further comprising a pump suitable for providing fluid transport to, through and from said cell.
Claim 11
Dependent Claim
14 words
An apparatus as recited in claim 9, wherein said pump is a peristatic pump.
Claim 12
Dependent Claim
15 words
An apparatus as recited in claim 9, wherein said pump is a positive displacement pump.
Claim 13
Dependent Claim
24 words
An apparatus as recited in claim 1, wherein said means to measure chemiluminescence does so by directly measuring chemiluminescence emitted at the collection zone.
Claim 14
Dependent Claim
23 words
An apparatus as recited in claim 12, further comprising a shutter mechanism provided between said cell and said means to measure the chemiluminescence.
Claim 15
Dependent Claim
39 words
An apparatus as recited in claim 12, wherein said shutter mechanism is controllably operated to open only so far as to expose the means to measure the chemiluminescence from the collection zone in the cell during chemiluminescence measurement periods.
Claim 16
Dependent Claim
26 words
An apparatus as recited in claim 12, further comprising a means for reflecting chemiluminescence generated at the collection zone onto said means to measure the chemiluminescence.
Claim 17
Dependent Claim
28 words
An apparatus as recited in claim 12, further comprising a housing enclosing said cell and shielding said means to measure the chemiluminescence from external light during chemiluminescent measurements.
Claim 18
Dependent Claim
18 words
An apparatus as recited in claim 12, wherein said means to measure the chemiluminescence is a photomultiplier tube.
Claim 19
Dependent Claim
22 words
An apparatus as recited in claim 12, further comprising a pump suitable for providing fluid transport to, through and from said cell.
Claim 20
Dependent Claim
14 words
An apparatus as recited in claim 18, wherein said pump is a peristatic pump.
Claim 21
Dependent Claim
15 words
An apparatus as recited in claim 18, wherein said pump is a positive displacement pump.
Claim 22
Dependent Claim
101 words
An assay apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising: (a) a cell consisting essentially of a sample volume having inlet means and outlet means and a single collection zone; (b) means for generating a magnetic field at said collection zone, wherein said means for generating said magnetic field is positioned proximate to said cell and said field is sufficient to collect said analyte at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (c) means to measure the chemiluminescence generated at the collection zone.
Claim 23
Dependent Claim
101 words
An assay apparatus for measurement of an analyte of interest based upon measurement of chemiluminescence comprising: (a) a cell consisting essentially of a sample volume having inlet means and outlet means and a single collection zone; (b) means for generating a magnetic field at said collection zone, wherein said means for generating said magnetic field is positioned proximate to said cell and said field is sufficient to collect said analyte at said collection zone when any such analyte of interest is bound directly or indirectly to a magnetic particle; and (c) means to measure the chemiluminescence generated at the collection zone.
Claim 24
Dependent Claim
24 words
An apparatus as recited in claim 21, wherein said means to measure chemiluminescence does so by directly measuring chemiluminescence emitted at the collection zone.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-08-24 | 2002-02-23 | 2002-08-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-24 | 2006-02-23 | 2006-08-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-24 | 2010-02-23 | 2010-08-25 | $7700.00 | Window Closed / Lapsed |