US5798030A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor membranes
Assignee: The University of Sydney
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Filing: 1997-04-09
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Granted: 1998-08-25
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Expires: 2017-04-09
Abstract
Abstract The present invention relates to a novel biosensor comprising an electrode membrane combination. This novel biosensor employs compounds include a linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode, the electrode being either in part or totally made up of the linker lipid. The linker lipid comprises within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, a hydrophilic region intermediate said hydrophobic region and the attachment group and a polar head group region attached to the hydrophobic region at a site remote from the hydrophilic region.
Inventors
- 👤 Burkhard Raguse
- 👤 Bruce A Cornell
- 👤 Vijoleta L Braach-Maksvytis
- 👤 Ronald J Pace
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3275; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
113 words
A biosensor for use in detecting the presence or absence of an analyte in a sample, the biosensor comprising an electrode and a bilayer membrane comprising a top layer and a bottom layer, the bottom layer being proximal to and connected to the electrode such that a space exists between the membrane and the electrode, the conductance of the membrane being dependent on the presence or absence of the analyte, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ionophores dispersed therein, and a layer of a hydrogel formed on top of the lipid bilayer membrane, the hydrogel allowing the passage of the analyte molecule to be detected.
Claim 2
Dependent Claim
113 words
A biosensor for use in detecting the presence or absence of an analyte in a sample, the biosensor comprising an electrode and a bilayer membrane comprising a top layer and a bottom layer, the bottom layer being proximal to and connected to the electrode such that a space exists between the membrane and the electrode, the conductance of the membrane being dependent on the presence or absence of the analyte, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ionophores dispersed therein, and a layer of a hydrogel formed on top of the lipid bilayer membrane, the hydrogel allowing the passage of the analyte molecule to be detected.
Claim 3
Dependent Claim
15 words
A biosensor as claimed in claim 1 in which the hydrogel is a thermosetting gel.
Claim 4
Dependent Claim
16 words
A biosensor as claimed in claim 2 in which the thermosetting gel is an agar gel.
Claim 5
Dependent Claim
15 words
A biosensor as claimed in claim 2 in which the gel contains between 0.3-5% agar.
Claim 6
Dependent Claim
16 words
A biosensor as claimed in claim 2 in which the thermosetting gel is a gelatine gel.
Claim 7
Dependent Claim
17 words
A biosensor as claimed in claim 1 in which the hydrogel is an in situ polymerised hydrogel.
Claim 8
Dependent Claim
27 words
A biosensor as claimed in claim 1 in which the hydrogel is an acrylic acid or an acrylic acid derivative that is polymerised by free radial polymerisation.
Claim 9
Dependent Claim
19 words
A biosensor as claimed in claim 1 in which the hydrogel is formed from acrylamide and a bisacrylamide cross-linker.
Claim 10
Dependent Claim
24 words
A biosensor as claimed in claim 1 in which the hydrogel is formed from cross-linked hydroxyethyl acrylate or hydroxyethyl methacrylate or other biocompatible gel.
Claim 11
Dependent Claim
40 words
A biosensor as claimed in claim 1 in which the ionophore is capable of transporting an ion that is produced by the reaction of an enzyme with its substrate, said enzyme being either covalently or non-covalently attached to the hydrogel.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-08-24 | 2002-02-23 | 2002-08-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-24 | 2006-02-23 | 2006-08-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-24 | 2010-02-23 | 2010-08-25 | $7700.00 | Window Closed / Lapsed |