US4661235A
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Chemo-receptive lipid based membrane transducers
Assignee: University of Toronto Innovations Foundation
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Filing: 1984-08-03
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Granted: 1987-04-28
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Expires: 2004-08-03
Abstract
Abstract A lipid membrane is used as a chemo-receptive transducer for quantitative and semi-quantitative analysis of chemical test species in an electrolyte. The lipid membrane is contacted with an electrolyte containing the test species, and an electrical potential difference is applied across the membrane. From measurements of the trans-membrane ion current variation due to the presence of the test species, a quantitative analysis of the test species may be conducted, on a highly selective basis. Receptors showing a high degree of selectivity towards the test species are included in the membrane.
Inventors
- 👤 Ulrich J. Krull
- 👤 Michael Thompson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 19:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/333; G01N27/3335; G01N33/92; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
73 words
A lipid membrane-based device that utilizes transmembrane ion movement for analyzing the concentration of a selected chemical species, said device comprising a perturbable, ion-permeable, lipid membrane and an ion conductive support on which is deposited said ion-permeable lipid membrane, said ion-permeable lipid membrane including a complexing agent for selectively interacting with said selected chemical species to increase transmembrane ion movement, and said ion-permeable lipid membrane having an increased transmembrane ion movement when perturbed.
Claim 2
Dependent Claim
73 words
A lipid membrane-based device that utilizes transmembrane ion movement for analyzing the concentration of a selected chemical species, said device comprising a perturbable, ion-permeable, lipid membrane and an ion conductive support on which is deposited said ion-permeable lipid membrane, said ion-permeable lipid membrane including a complexing agent for selectively interacting with said selected chemical species to increase transmembrane ion movement, and said ion-permeable lipid membrane having an increased transmembrane ion movement when perturbed.
Claim 3
Dependent Claim
17 words
The supported, membrane-based device of claim 1, wherein said ion conductive support is a stable gel substrate.
Claim 4
Dependent Claim
15 words
The supported, membrane-based device of claim 1, wherein said ion conductive support is a hydrogel.
Claim 5
Dependent Claim
18 words
The supported, membrane-based device of claim 3, wherein said hydrogel is physically stabilized on an electrochemical reference electrode.
Claim 6
Dependent Claim
14 words
The supported, membrane-based device of claim 4, wherein said hydrogel is a polyacrylamide hydrogel.
Claim 7
Dependent Claim
16 words
The supported, membrane-based device of claim 1, wherein said lipid membrane is a bilayer lipid membrane.
Claim 8
Dependent Claim
16 words
The supported, membrane-based device of claim 1, wherein said lipid membrane is a monolayer lipid membrane.
Claim 9
Dependent Claim
19 words
The supported, membrane-based device of claim 1, wherein said complexing agent is a receptor selective for an organic compound.
Claim 10
Dependent Claim
14 words
The supported, membrane-based device of claim 8, wherein said receptor is a natural product.
Claim 11
Dependent Claim
16 words
The supported, membrane-based device of claim 8, wherein said receptor is chemically bound in the membrane.
Claim 12
Dependent Claim
17 words
The supported, membrane-based device of claim 1, wherein said complexing agent is selective for an inorganic ion.
Claim 13
Dependent Claim
64 words
A gas-sensor electrochemical cell comprising (a) a gas-permeable membrane permeable to an inorganic ion-forming gas, and (b) a perturbable, ion-permeable lipid membrane including a complexing agent for selectively interacting with a selected inorganic ion formed by dissolution of said inorganic ion-forming gas in an aqueous electrolytic solution, said gas-permeable membrane being separated from said ion-permeable lipid membrane by the lipid membrane-contacting, aqueous electrolytic solution.
Claim 14
Dependent Claim
64 words
A gas-sensor electrochemical cell comprising (a) a gas-permeable membrane permeable to an inorganic ion-forming gas, and (b) a perturbable, ion-permeable lipid membrane including a complexing agent for selectively interacting with a selected inorganic ion formed by dissolution of said inorganic ion-forming gas in an aqueous electrolytic solution, said gas-permeable membrane being separated from said ion-permeable lipid membrane by the lipid membrane-contacting, aqueous electrolytic solution.
Claim 15
Dependent Claim
14 words
The gas-sensor electrochemical cell of claim 12, wherein said complexing agent is a polypeptide.
Claim 16
Dependent Claim
23 words
The gas-sensor electrochemical cell of claim 13, wherein said polypeptide is an antibiotic selected from the group consisting of gramicidin, valinomycin and nonactin.
Claim 17
Dependent Claim
12 words
The gas-sensor electrochemical cell of claim 13, wherein said polypeptide is nonactin.
Claim 18
Dependent Claim
15 words
The gas-sensor electrochemical cell of claim 12, wherein said gas-permeable membrane is a polytetrafluoroethylene membrane.
Claim 19
Dependent Claim
16 words
The gas-sensor electrochemical cell of claim 12, wherein said modified membrane is a bilayer lipid membrane.
Claim 20
Dependent Claim
16 words
The gas-sensor electrochemical cell of claim 12, wherein said modified membrane is a monolayer lipid membrane.
References Cited (Backward Citations)
US3607700A
Cited
US4490216A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-27 | 1990-10-27 | 1991-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-27 | 1994-10-27 | 1995-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-27 | 1998-10-27 | 1999-04-28 | $7700.00 | Window Closed / Lapsed |