US5789228A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Endoglucanases
Assignee: BASF Enzymes LLC
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Filing: 1996-05-22
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Granted: 1998-08-04
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Expires: 2016-05-22
Abstract
Abstract A purified thermostable enzyme is derived from the archael bacterium AEPII1a. The enzyme has a molecular weight of about 60.9 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of cellulose where desired.
Inventors
- 👤 David E. Lam
- 👤 Eric J. Mathur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:33
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N9/2445; C12P; C12P19/00; C12P19/14; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12Y302/01021
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
13 words
An isolated polynucleotide sequence encoding the amino acid sequence of SEQ ID NO:2.
Claim 2
Dependent Claim
13 words
An isolated polynucleotide sequence encoding the amino acid sequence of SEQ ID NO:2.
Claim 3
Dependent Claim
13 words
The polynucleotide of claim 1, wherein the polynucleotide is isolated from a prokaryote.
Claim 4
Dependent Claim
9 words
An expression vector containing the polynucleotide of claim 1.
Claim 5
Dependent Claim
11 words
The vector of claim 3, wherein the vector is a plasmid.
Claim 6
Dependent Claim
10 words
The vector of claim 3, wherein the vector is virus-derived.
Claim 7
Dependent Claim
33 words
A process for producing a cell comprising transforming or transfecting the cell with the vector of claim 3 such that the cell expresses the polypeptide encoded by the DNA contained in the vector.
Claim 8
Dependent Claim
11 words
A host cell stably transformed with the vector of claim 3.
Claim 9
Dependent Claim
13 words
The host cell of claim 7, wherein the cell is a prokaryotic cell.
Claim 10
Dependent Claim
13 words
The host cell of claim 7, wherein the cell is a eukaryotic cell.
Claim 11
Dependent Claim
35 words
A method for producing a polypeptide comprising: a) culturing the host cell of claim 7; b) expressing from the host cell of claim 7 a polypeptide encoded by said DNA; and c) isolating the polypeptide.
Claim 12
Dependent Claim
52 words
An isolated polynucleotide selected from the group consisting of: a) SEQ ID NO:1; b) SEQ ID NO:1 wherein T can also be U; c) nucleic acid sequences complementary to a) and b); and d) polynucleotides contained in ATCC deposit No. 97516 and which encode the amino acid sequence of SEQ ID NO:2.
Claim 13
Dependent Claim
52 words
An isolated polynucleotide selected from the group consisting of: a) SEQ ID NO:1; b) SEQ ID NO:1 wherein T can also be U; c) nucleic acid sequences complementary to a) and b); and d) polynucleotides contained in ATCC deposit No. 97516 and which encode the amino acid sequence of SEQ ID NO:2.
References Cited (Backward Citations)
US5393670A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-08-03 | 2002-02-02 | 2002-08-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-03 | 2006-02-02 | 2006-08-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-03 | 2010-02-02 | 2010-08-04 | $7700.00 | Window Closed / Lapsed |