Patent US5780294A successfully refreshed from Google Patents.
US5780294A
Expired / Lapsed
US
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Culture vessel assembly
Assignee: Corning Inc
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Filing: 1997-03-19
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Granted: 1998-07-14
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Expires: 2017-03-19
Abstract
Abstract A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for accommodating a cell culture insert and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured in the cell culture insert. The means for varying the gas diffusion is a gas permeable membrane provided in a plurality of openings in the lid and a plurality of removable thin impermeable film labels are provided for selectively occluding passage of gases through the gas permeable membranes.
Inventors
- 👤 Timothy A. Stevens
- 👤 Tadeusz A. Tyndorf
- 👤 Susan L. Barker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:48
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/02; C12M25/04; C12M23/00; C12M23/02; C12M23/12; C12M23/24; C12M23/38; C12M29/00; C12M29/04
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
123 words
An assembly comprising: a test plate comprising a plurality of wells having openings therein for receiving tissue culture media or cell culture inserts and upstanding sidewall forming an outside border of said plate; a lid removably positioned on said plate comprising a substantially planar cover extending over said wells, comprising an upper surface and a lower surface, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly in spaced relation with respect to said sidewall of said plate, to form sidewalls having an inner and outer surface; and a plurality of orifices in said planar cover each comprising a gas permeable membrane and positioned whereby said orifices are in alignment with said wells of said test plate.
Claim 2
Dependent Claim
123 words
An assembly comprising: a test plate comprising a plurality of wells having openings therein for receiving tissue culture media or cell culture inserts and upstanding sidewall forming an outside border of said plate; a lid removably positioned on said plate comprising a substantially planar cover extending over said wells, comprising an upper surface and a lower surface, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly in spaced relation with respect to said sidewall of said plate, to form sidewalls having an inner and outer surface; and a plurality of orifices in said planar cover each comprising a gas permeable membrane and positioned whereby said orifices are in alignment with said wells of said test plate.
Claim 3
Dependent Claim
40 words
The assembly of claim 1 further comprising gas impermeable films removably attached to said cover and associated with each orifice whereby said film may be fully or partially removed to assist in gas diffusion into and out of said assembly.
Claim 4
Dependent Claim
23 words
The assembly of claim 1 wherein said sidewall of said plate comprises a two-step section comprising an upper section and a lower section.
Claim 5
Dependent Claim
22 words
The assembly of claim 3 wherein said lower section of said sidewall is stepped from said upper section by an annular shoulder.
Claim 6
Dependent Claim
28 words
The assembly of claim 1 wherein said skirt of said lid extends to a position adjacent to, but out of contact with, said annular shoulder of said plate.
Claim 7
Dependent Claim
16 words
The assembly of claim 1 wherein said wells are arranged in substantially orthogonal rows and columns.
Claim 8
Dependent Claim
16 words
The assembly of claim 1 wherein said plate and said lid are made of transparent plastic.
Claim 9
Dependent Claim
47 words
The assembly of claim 1 wherein said test plate further comprises a ledge spaced inwardly from said sidewall of said plate and extending between said sidewalls and said wells wherein said sidewalls extend to a position raised above said ledge thereby forming a rim surrounding said ledge.
Claim 10
Dependent Claim
90 words
A lid for use in conjunction with a tissue culture plate comprising: a substantially planar cover comprising an upper surface and a lower surface; a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly to form sidewalls having an inner and outer surface; a plurality of orifices in said cover each comprising a gas permeable membrane; and gas impermeable films removably attached to said cover over each of said orifices and said gas permeable membranes whereby each film may be fully or partially removed.
Claim 11
Dependent Claim
90 words
A lid for use in conjunction with a tissue culture plate comprising: a substantially planar cover comprising an upper surface and a lower surface; a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly to form sidewalls having an inner and outer surface; a plurality of orifices in said cover each comprising a gas permeable membrane; and gas impermeable films removably attached to said cover over each of said orifices and said gas permeable membranes whereby each film may be fully or partially removed.
Claim 12
Dependent Claim
13 words
The lid of claim 9 wherein said gas impermeable films are peel-away labels.
Claim 13
Dependent Claim
27 words
The lid of claim 10 further comprising a plurality of flanges being inwardly spaced from said skirt and extending downwardly from said lower surface of said cover.
Claim 14
Independent Claim
93 words
A device for growing cells or tissue culture in vitro comprising: a body comprising an upper surface, a lower surface, a plurality of wells substantially disposed between said upper and lower surface and upstanding sidewall forming an outside border of said body; and a removable lid, comprising a top wall and a peripheral skirt that extends from said top wall and a plurality of means for allowing gas diffusion into and out of said device at said top wall, wherein said plurality of means is in alignment with said wells of said body.
Claim 15
Independent Claim
93 words
A device for growing cells or tissue culture in vitro comprising: a body comprising an upper surface, a lower surface, a plurality of wells substantially disposed between said upper and lower surface and upstanding sidewall forming an outside border of said body; and a removable lid, comprising a top wall and a peripheral skirt that extends from said top wall and a plurality of means for allowing gas diffusion into and out of said device at said top wall, wherein said plurality of means is in alignment with said wells of said body.
Claim 16
Dependent Claim
43 words
The device of claim 12 wherein said plurality of means for allowing gas diffusion into and out of the device is a plurality of orifices and gas impermeable membranes associated with said orifices and in direct alignment with said wells of said body.
Claim 17
Dependent Claim
38 words
The device of claim 13 further comprising a plurality of gas impermeable films removably attached to said cover over said orifices, whereby said films may be fully or partially removed to assist with gas diffusion in said device.
Claim 18
Dependent Claim
24 words
The device of claim 12 wherein said sidewall of said body includes a two step section comprising an upper section and a lower section.
Claim 19
Dependent Claim
30 words
The device of claim 15 wherein said sidewall of said body further comprises an annular shoulder whereby said lower section is stepped from said upper section by said annular shoulder.
Claim 20
Independent Claim
214 words
A method of culturing cells comprising: (a) providing a culture vessel comprising a test plate comprising a plurality of wells having openings therein for receiving tissue culture media and upstanding sidewall forming an outside border of said plate; a lid removably positioned on said plate comprising a substantially planar cover extending over said wells, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly to form sidewalls having an inner and outer surface; a plurality of orifices in said planar cover each comprising a gas permeable membrane wherein said orifices are in direct alignment with said wells of said plate; and removable labels associated with said orifices to cover said membrane; (b) removing said lid from said culture vessel; (c) placing cell inserts comprising a membrane within said wells of said culture vessel; (d) depositing a layer of cells on each membrane of said cell insert within said culture vessel; (e) overlaying the layer of cells with a growth medium; (f) placing said lid on said plate to cover said wells; and (g) directly ventilating said membrane of each cell insert by permitting air access thrusts through said orifices in said lid of said culture vessel by fully or partially removing and re-attaching said labels over said orifices.
Claim 21
Independent Claim
214 words
A method of culturing cells comprising: (a) providing a culture vessel comprising a test plate comprising a plurality of wells having openings therein for receiving tissue culture media and upstanding sidewall forming an outside border of said plate; a lid removably positioned on said plate comprising a substantially planar cover extending over said wells, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly to form sidewalls having an inner and outer surface; a plurality of orifices in said planar cover each comprising a gas permeable membrane wherein said orifices are in direct alignment with said wells of said plate; and removable labels associated with said orifices to cover said membrane; (b) removing said lid from said culture vessel; (c) placing cell inserts comprising a membrane within said wells of said culture vessel; (d) depositing a layer of cells on each membrane of said cell insert within said culture vessel; (e) overlaying the layer of cells with a growth medium; (f) placing said lid on said plate to cover said wells; and (g) directly ventilating said membrane of each cell insert by permitting air access thrusts through said orifices in said lid of said culture vessel by fully or partially removing and re-attaching said labels over said orifices.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-07-13 | 2002-01-12 | 2002-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-07-13 | 2006-01-12 | 2006-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-07-13 | 2010-01-12 | 2010-07-14 | $7700.00 | Window Closed / Lapsed |