US5780052A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Compositions and methods useful for inhibiting cell death and for delivering an agent into a cell
Assignee: Northeastern University
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Filing: 1995-04-24
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Granted: 1998-07-14
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Expires: 2015-04-24
Abstract
Abstract The invention relates to methods of salvaging a target cell from cell death, comprising contacting a target cell having a disrupted cell membrane with a specific affinity reagent-liposome conjugate in an amount effective and for a time sufficient to allow the conjugate to prevent cell death due to membrane disruption; and determining the viability of the target cell. Methods of delivering a selected agent into a damaged target cell for diagnosis and therapy are also disclosed.
Inventors
- 👤 Ban An Khaw
- 👤 Vladmir P. Torchilin
- 👤 Jagat Narula
- 👤 Imran Vural
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:11
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6835; A61K47/6843; A61K47/69; A61K47/6905; A61K47/6911; A61K47/6913; Y; Y10; Y10S; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
71 words
A method of inhibiting cell death in cardiac tissue, said method comprising providing cardiac tissue comprising injured cardiomyocytes having intracellular myosin exposed to the exterior of said cardiomyocytes; contacting said injured cardiomyocytes with a specific affinity reagent-liposome conjugate, said specific affinity reagent comprising an antibody specific for said intracellular myosin, for a time sufficient to allow said conjugate to adhere to said intracellular myosin; and determining the viability of said cardiomyocytes.
Claim 2
Independent Claim
71 words
A method of inhibiting cell death in cardiac tissue, said method comprising providing cardiac tissue comprising injured cardiomyocytes having intracellular myosin exposed to the exterior of said cardiomyocytes; contacting said injured cardiomyocytes with a specific affinity reagent-liposome conjugate, said specific affinity reagent comprising an antibody specific for said intracellular myosin, for a time sufficient to allow said conjugate to adhere to said intracellular myosin; and determining the viability of said cardiomyocytes.
Claim 3
Dependent Claim
12 words
The method of claim 1, wherein said conjugate comprises a biological agent.
Claim 4
Dependent Claim
32 words
The method of claim 2, wherein said biological agent is selected from the group consisting of fibroblastic growth factor-β, angiogenic factors, high energy substrates for the myocardium, antioxidants, cytokines and contrast agents.
References Cited (Backward Citations)
US4957735A
Cited
US5230900A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-07-13 | 2002-01-12 | 2002-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-07-13 | 2006-01-12 | 2006-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-07-13 | 2010-01-12 | 2010-07-14 | $7700.00 | Window Closed / Lapsed |