US5777201A
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US
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Modification of vegetable oils using desaturase
Assignee: Agrigenetics Inc
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Filing: 1996-10-31
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Granted: 1998-07-07
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Expires: 2016-10-31
Abstract
Abstract Plant seed having yeast delta-9 desaturase gene therein, preferably in association with a suitable promoter and termination sequence. A method for modifying the fatty acid content of seed oil, by transforming seed with yeast delta-9 desaturase.
Inventors
- 👤 Candace Gloria Poutre
- 👤 Asha Mchra-Palta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:21
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N9/0083; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8247; C12Y; C12Y114/00; C12Y114/19; C12Y114/19002
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
19 words
A plant seed comprising DNA encoding yeast delta-9 desaturase and means for expressing said DNA in said plant seed.
Claim 2
Dependent Claim
19 words
A plant seed comprising DNA encoding yeast delta-9 desaturase and means for expressing said DNA in said plant seed.
Claim 3
Dependent Claim
27 words
The plant seed as defined by claim 1, wherein said means for expressing comprises a promoter effective to cause expression of said DNA in said plant seed.
Claim 4
Dependent Claim
15 words
The plant seed as defined by claim 2, wherein said promoter is a seed-specific promoter.
Claim 5
Dependent Claim
16 words
The plant seed as defined by claim 1, further comprising a termination sequence for said DNA.
Claim 6
Dependent Claim
40 words
The plant seed as defined by claim 4, wherein said termination sequence is selected from the group consisting of a yeast delta-9 desaturase termination sequence (SEO ID NO:1), a phaseolin 3' termination sequence, and an ORF 25 3' termination sequence.
Claim 7
Dependent Claim
20 words
The plant seed as defined by claim 1, wherein said plant seed is a seed of a monocot plant genus.
Claim 8
Dependent Claim
23 words
The plant seed as defined by claim 6, wherein said monocot plant genus is selected from the group consisting of Zea and Sorghum.
Claim 9
Dependent Claim
20 words
The plant seed as defined by claim 1, wherein said plant seed is a seed of a dicot plant genus.
Claim 10
Dependent Claim
30 words
The plant seed as defined by claim 8, wherein said dicot plant genus is selected from the group consisting of Brassica, Helianthus, Carthamus, Sesamum, Glycine, Arachis, Gossypium, Lesquerella, and Vernonia.
Claim 11
Dependent Claim
27 words
The plant seed as defined by claim 9, further wherein said plant seed is a seed selected from the group consisting of Brassica rapa and Brassica napus.
Claim 12
Independent Claim
55 words
A method for providing a modified fatty acid content of the seed oil of a plant seed, said method comprising the step of providing a transgenic plant seed comprising DNA encoding yeast delta-9 desaturase, wherein said DNA is expressed, whereby the fatty acid content is modified relative to the seed oil of non-transgenic plant seed.
Claim 13
Independent Claim
55 words
A method for providing a modified fatty acid content of the seed oil of a plant seed, said method comprising the step of providing a transgenic plant seed comprising DNA encoding yeast delta-9 desaturase, wherein said DNA is expressed, whereby the fatty acid content is modified relative to the seed oil of non-transgenic plant seed.
Claim 14
Dependent Claim
29 words
The method as defined by claim 11, wherein said modification comprises an increase in the percent content of monounsaturated fatty acid in the seed oil of said plant seed.
Claim 15
Dependent Claim
24 words
The method as defined by claim 12, wherein said monounsaturated fatty acid has a carbon chain length of from 16 to 24 carbon atoms.
Claim 16
Dependent Claim
46 words
The method as defined by claim 13, wherein said monounsaturated fatty acid is selected from the group consisting of cis-9-hexadecanoic acid (palmitoleic acid), cis-9-octadecanoic acid (oleic acid), cis-11-octadecenoic acid (cis-vaccenic acid), cis-11-eicosenoic acid, cis-13-eicosenoic acid, cis-13-docosenoic acid, cis-15-docosenoic acid, cis-15-tetracosenoic acid, cis-17-tetracosenoic acid, and combinations thereof.
Claim 17
Dependent Claim
29 words
The method as defined by claim 11, wherein said modification comprises a reduction in the percent content of saturated fatty acid in the seed oil of said plant seed.
Claim 18
Dependent Claim
34 words
The method as defined by claim 15, wherein said saturated fatty acid is selected from the group consisting of myristic acid, palmitic acid, stearic acid, eicosanoic acid, docosanoic acid, tetracosanoic acid, and combinations thereof.
Claim 19
Dependent Claim
19 words
The method as defined by claim 15, wherein said plant seed is a seed of a monocot plant genus.
Claim 20
Dependent Claim
22 words
The method as defined by claim 17, wherein said monocot plant genus is selected from the group consisting of Zea and Sorghum.
Claim 21
Dependent Claim
19 words
The method as defined by claim 11, wherein said plant seed is a seed of a dicot plant genus.
Claim 22
Dependent Claim
30 words
The method seed as defined by claim 19, wherein said dicot plant genus is selected from the group consisting of Brassica, Helianthus, Carthamus, Sesamum, Glycine, Arachis, Gossypium, Lesquerella, and Vernonia.
Claim 23
Dependent Claim
26 words
The method as defined by claim 20, further wherein said plant seed is a seed selected from the group consisting of Brassica rapa and Brassica napus.
Claim 24
Dependent Claim
48 words
The method as defined by claim 11, wherein said step of providing comprises transforming a plant cell to contain exogenous DNA encoding yeast delta-9 desaturase, culturing said cell under conditions whereby a plant is regenerated therefrom, and maintaining said plant under conditions whereby said plant produces transgenic seed.
Claim 25
Dependent Claim
37 words
The method as defined by claim 22, wherein said step of transforming is carried out using transformation mediation selected from the group consisting of Agrobacterium, electroporation, polyethylene glycol (PEG), silicon carbide fiber, particle gun, and direct injection.
Claim 26
Dependent Claim
68 words
The method as defined by claim 23, further comprising constructing a vector containing DNA encoding yeast delta-9 desaturase and a promoter, placing said vector into a selected strain of Agrobacterium, and treating selected plant cells with said Agrobacterium under conditions sufficient to result in transfer of at least some of said vectors from said Agrobacterium to said plant cells, whereby said DNA is expressed in said plant cells.
Claim 27
Dependent Claim
35 words
A plant obtained from the plant seed as defined by claim 1, wherein the seeds of said plant comprise a DNA encoding yeast delta-9 desaturase and means for expressing said DNA in said plant seed.
References Cited (Backward Citations)
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