US5057419A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Genetically engineered plasmid and organisms for the production of specialized oils
Assignee: Rutgers, The State University of New Jersey
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Filing: 1988-09-22
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Granted: 1991-10-15
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Expires: 2008-09-22
Abstract
Abstract A DNA fragment containing a gene encoding a fatty acid desaturase enzyme is isolated. The gene is then employed by introducing it into other cells to alter or enhance the production of oils in such cells, alternatively the gene or nucleic acid sequences of the gene can be employed to isolate other genes encoding for related, but different desaturase enzymes.
Inventors
- 👤 Charles E. Martin
- 👤 Robert M. Johnston
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:18
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N9/0083; C07; C07K; C07K16/00; C07K16/40; C12P; C12P7/00; C12P7/64; C12P7/6436; C12P7/6445; C12P7/6463; C12Y; C12Y114/00; C12Y114/19; C12Y114/19002
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
26 words
An expression vector comprising a yeast gene encoding a delta-9 fatty acid desaturase enzyme which functions in a yeast cell to induce or enhance oil production.
Claim 2
Dependent Claim
26 words
An expression vector comprising a yeast gene encoding a delta-9 fatty acid desaturase enzyme which functions in a yeast cell to induce or enhance oil production.
Claim 3
Dependent Claim
13 words
The expression vector recited in claim 1 wherein the oil is a triglyceride.
Claim 4
Dependent Claim
16 words
The expression vector recited in claim 1 wherein the expression vector comprises a HindIII restriction site.
Claim 5
Dependent Claim
19 words
The expression vector recited in claim 1 wherein the gene is isolated from yeast as a HindIII restriction fragment.
Claim 6
Dependent Claim
17 words
The expression vector recited in claim 1 wherein said expression vector contains multiple copies of said gene.
Claim 7
Dependent Claim
18 words
The expression vector recited in claim 1 wherein said expression vector further comprises a promoter for said gene.
Claim 8
Dependent Claim
14 words
The expression vector recited in claim 6 wherein said promoter is a GAL promoter.
Claim 9
Dependent Claim
12 words
A genetically engineered yeast organism transformed by the vector of claim 1.
Claim 10
Dependent Claim
12 words
The organism recited in claim 8 having multiple copies of the vector.
Claim 11
Dependent Claim
19 words
The organism recited in claim 8 wherein said gene is contained in a restriction fragment obtained from yeast cells.
Claim 12
Dependent Claim
14 words
The organism recited in claim 10 wherein said restriction fragment is a HindIII fragment.
Claim 13
Dependent Claim
23 words
The organism recited in claim 8 comprising a vector comprising an added gene encoding a delta-9 fatty acid desaturase enzyme and a promoter.
Claim 14
Dependent Claim
13 words
The organism recited in claim 12 wherein said promoter is a GAL promoter.
Claim 15
Dependent Claim
11 words
A purified DNA comprising the sequence as described in Table 2.
Claim 16
Dependent Claim
11 words
A purified DNA comprising the sequence as described in Table 2.
Claim 17
Dependent Claim
15 words
A yeast organism transformed with a DNA comprising the sequence set forth in Table 2.
Claim 18
Dependent Claim
15 words
A yeast organism transformed with a DNA comprising the sequence set forth in Table 2.
Claim 19
Dependent Claim
10 words
The organism according to claim 15 which is ATCC 74039.
Claim 20
Independent Claim
45 words
A method for producing oil in yeast comprising culturing a yeast stain tranformed with an expression vector comprising a gene encoding a delta-9 fatty acid desaturase enzyme in a medium under conditions of time and temperature sufficient to result in oil production by said yeast.
Claim 21
Independent Claim
45 words
A method for producing oil in yeast comprising culturing a yeast stain tranformed with an expression vector comprising a gene encoding a delta-9 fatty acid desaturase enzyme in a medium under conditions of time and temperature sufficient to result in oil production by said yeast.
Claim 22
Dependent Claim
12 words
The method according to claim 17 wherein said yeast is Saccharomyces cerevisae.
Claim 23
Dependent Claim
12 words
The method according to claim 18 wherein said yeast is ATCC 74039.
Claim 24
Dependent Claim
19 words
The method according to claim 17 which includes the further steps of recovering said oil from said yeast culture.
References Cited (Backward Citations)
US4661454A
Cited
US4771002A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-14 | 1995-04-15 | 1995-10-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-14 | 1999-04-15 | 1999-10-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-14 | 2003-04-15 | 2003-10-15 | $7700.00 | Window Closed / Lapsed |