US5760004A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chemical modification of repetitive polymers to enhance water solubility
Assignee: Protein Polymer Technologies Inc
•
Filing: 1994-11-21
•
Granted: 1998-06-02
•
Expires: 2014-11-21
Abstract
Abstract Highly repetitive proteins which are relatively insoluble in water are chemically modified to increase solubility. The protein is reacted with a functionalizing agent to introduce additional polar functionalities and disrupt the order of the protein. The solubility of the protein in water is increased by the chemical modification, while adhesive and surfactant properties are retained.
Inventors
- 👤 Erwin R. Stedronsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:05
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/1072; C07K14/00; C07K14/001; C07K14/435
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
103 words
A water soluble repetitive unit protein compound of at least 6 kD comprising a repetitive unit having the amino acid sequence GAGAGS, wherein said repetitive unit constitutes at least 5 number % of the total amino acids of which at least about 1% are bonded to a polar group substituted alkyl group of from 2 to 8 carbon atoms, where the protein without said polar group substituted alkyl group has a water solubility of less than 1.0 mg/ml at ambient conditions and wherein said polar group substituted alkyl group is bonded to the amino acids of said protein after said protein is synthesized.
Claim 2
Dependent Claim
103 words
A water soluble repetitive unit protein compound of at least 6 kD comprising a repetitive unit having the amino acid sequence GAGAGS, wherein said repetitive unit constitutes at least 5 number % of the total amino acids of which at least about 1% are bonded to a polar group substituted alkyl group of from 2 to 8 carbon atoms, where the protein without said polar group substituted alkyl group has a water solubility of less than 1.0 mg/ml at ambient conditions and wherein said polar group substituted alkyl group is bonded to the amino acids of said protein after said protein is synthesized.
Claim 3
Dependent Claim
26 words
The water soluble repetitive unit protein compound according to claim 1, wherein said polar group is selected from the group consisting of hydroxyl, sulfonato and ammonio.
Claim 4
Dependent Claim
17 words
The water soluble repetitive unit protein compound according to claim 1, wherein said polar group is hydroxyl.
Claim 5
Dependent Claim
24 words
The water soluble repetitive unit protein compound according to claim 1, wherein said repetitive units have an intervening sequence comprising a physiologically active sequence.
Claim 6
Dependent Claim
22 words
The water soluble repetitive unit protein compound according to claim 4, wherein said physiologically active sequence comprises the amino acid sequence RGDS.
Claim 7
Dependent Claim
17 words
The water soluble repetitive unit protein compound according to claim 1, wherein said protein is SLPF (ProNectin®-F).
Claim 8
Dependent Claim
73 words
A water soluble repetitive unit protein compound of at least 6 kD comprising a repetitive unit having the amino acid sequence GAGAGS, wherein at least about 1% of the serine residues in said repetitive units are bonded to a polar group substituted alkyl group of from 2 to 8 carbon atoms, where the protein without said polar group substituted alkyl group has a water solubility of less than 1.0 mg/ml at ambient conditions.
Claim 9
Dependent Claim
73 words
A water soluble repetitive unit protein compound of at least 6 kD comprising a repetitive unit having the amino acid sequence GAGAGS, wherein at least about 1% of the serine residues in said repetitive units are bonded to a polar group substituted alkyl group of from 2 to 8 carbon atoms, where the protein without said polar group substituted alkyl group has a water solubility of less than 1.0 mg/ml at ambient conditions.
Claim 10
Dependent Claim
26 words
The water soluble repetitive unit protein compound according to claim 7, wherein said polar group is selected from the group consisting of hydroxyl, sulfonato and ammonio.
Claim 11
Dependent Claim
17 words
The water soluble repetitive unit protein compound according to claim 7, wherein said polar group is hydroxyl.
Claim 12
Dependent Claim
24 words
The water soluble repetitive unit protein compound according to claim 7, wherein said repetitive units have an intervening sequence comprising a physiologically active sequence.
Claim 13
Dependent Claim
22 words
The water soluble repetitive unit protein compound according to claim 10, wherein said physiologically active sequence comprises the amino acid sequence RGDS.
Claim 14
Dependent Claim
18 words
The water soluble repetitive unit protein according to claim 7, wherein said protein is silk-like protein III (SLP3.0).
Claim 15
Dependent Claim
28 words
An aqueous solution having less than 1M salt concentration and at least 0.01 weight % of a protein compound according to any one of claims 1 or 7.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-01 | 2001-12-01 | 2002-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-01 | 2005-12-01 | 2006-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-01 | 2009-12-01 | 2010-06-02 | $7700.00 | Window Closed / Lapsed |