US5759769A
Expired / Lapsed
US
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Tandem synthetic HIV-1 peptides
Assignee: Sanofi Pasteur Ltd
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Filing: 1995-06-02
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Granted: 1998-06-02
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Expires: 2015-06-02
Abstract
Abstract Novel synthetic peptides are provided which are candidate vaccines against HIV-1 and which are useful in diagnostic application. The peptides comprise an amino acid sequence of a T-cell epitope of the gag protein of HIV-1, specifically p24E linked directly to an amino acid sequence of a B-cell epitope of the V3 loop protein of an HIV-1 isolate and containing the sequence GPGR, and/or the gp41 containing the sequence ELKDWA. Multimeric forms of the tandem synthetic peptides are provided.
Inventors
- 👤 Charles D. Y. Sia
- 👤 Pele Chong
- 👤 Michel H. Klein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:38
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/08; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; A61K39/12; A61K39/21; A61P; A61P31/00; A61P31/12; A61P31/14; A61P31/18; A61P37/00; A61P37/02; A61P37/04; C07K14/08; C07K14/15; C07K14/155; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; C07K2319/00; C12; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16022; C12N2740/16034; C12N2740/16111; C12N2740/16122; C12N2740/16211; C12N2740/16222; Y; Y10; Y10S; Y10S977/00; Y10S977/70; Y10S977/788; Y10S977/802; Y10S977/902; Y10S977/904; Y10S977/915; Y10S977/917; Y10S977/92
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
526 words
A diagnostic kit useful for detecting HIV specific antibodies in a test sample, the kit comprising: (a) a surface; (b) at least one peptide immobilized on the surface and having an amino acid sequence epitopically specific for the HIV-specific antibodies, wherein said peptide is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate and is selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70), EEMMTACOGVGGPGHK (SEQ ID NO:73), GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVQNIQGQMVHQAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which is a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequences each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequences; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate; and (c) reagent for detecting a complex formed between HIV specific antibodies in the test sample and the at least one immobilized peptide.
Claim 2
Dependent Claim
526 words
A diagnostic kit useful for detecting HIV specific antibodies in a test sample, the kit comprising: (a) a surface; (b) at least one peptide immobilized on the surface and having an amino acid sequence epitopically specific for the HIV-specific antibodies, wherein said peptide is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate and is selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70), EEMMTACOGVGGPGHK (SEQ ID NO:73), GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVQNIQGQMVHQAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which is a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequences each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequences; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate; and (c) reagent for detecting a complex formed between HIV specific antibodies in the test sample and the at least one immobilized peptide.
Claim 3
Dependent Claim
522 words
A diagnostic kit for detecting HIV antigens in a test sample, the kit comprising: (a) a surface; (b) an antibody immobilized on the surface and epitopically specific and noncross-reactive for distinct epitopes of the HIV antigen and raised to a peptide which is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70), EEMMTACOGVGGPGHK (SEQ ID NO:73), GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVONIOGOMVHOAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which contains a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence comprising a B-cell epitope which contains a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequence each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequence; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate; and (c) reagent for detecting a complex formed between HIV antigens in the test sample and the at least one immobilized antibody.
Claim 4
Dependent Claim
522 words
A diagnostic kit for detecting HIV antigens in a test sample, the kit comprising: (a) a surface; (b) an antibody immobilized on the surface and epitopically specific and noncross-reactive for distinct epitopes of the HIV antigen and raised to a peptide which is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70), EEMMTACOGVGGPGHK (SEQ ID NO:73), GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVONIOGOMVHOAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which contains a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence comprising a B-cell epitope which contains a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequence each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequence; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate; and (c) reagent for detecting a complex formed between HIV antigens in the test sample and the at least one immobilized antibody.
Claim 5
Dependent Claim
465 words
An antibody specific for a peptide which is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70) EEMMTACOGVGGPGHK (SEQ ID NO:73) GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVONIOGOMVHOAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which contains a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequences each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequence; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate.
Claim 6
Dependent Claim
465 words
An antibody specific for a peptide which is selected from the group consisting of: (i) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate selected from the group consisting of P24N, P24L, P24M and P24H having the respective amino acid sequences OMREPRGSDIAGTTSTL (SEQ ID NO:70) EEMMTACOGVGGPGHK (SEQ ID NO:73) GHKARVLAEAMSQVT (SEQ ID NO:76) and PIVONIOGOMVHOAI (SEQ ID NO:79) linked at the C-terminal end of said T-cell epitope, to at least one amino acid sequence which contains a B-cell epitope of the V3 loop of the envelope protein of an HIV isolate, (ii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a hybrid V3 loop sequence from at least two HIV-1 isolates; (iii) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof to at least one amino acid sequence which contains a B-cell epitope comprising a consensus sequence of the V3 loop of at least two HIV-1 primary isolates; (iv) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolates linked at the C-terminal end thereof to at least two amino acid sequences each containing a B-cell epitope, said B-cell epitope containing amino acid sequences each comprising a V3 loop sequence from a different HIV-1 isolate or HIV-isolate consensus sequence; (v) a synthetic peptide, which comprises at least one amino acid sequence which contains a T-cell epitope of the gag protein of a human immunodeficiency virus (HIV) isolate linked at the C-terminal end thereof, to at least one amino acid sequence which contains a B-cell epitope of the gp41 protein of an HIV isolate comprising the amino acid sequence X 1 LKDWX 2 wherein X 1 is E, A, G or Q and X 2 is A or T or an amino acid sequence capable of eliciting an HIV-specific antiserum and recognizing the amino acid sequence X 1 LKDWX 2 ; and (vi) a synthetic peptide, which comprises a plurality of individual synthetic peptides linked to form a multimeric molecule, each said individual synthetic peptide comprising an amino acid sequence which contains a T-cell epitope of a gag or envelope protein of a human immunodeficiency virus (HIV) isolate linked to an amino acid sequence which contains a B-cell epitope of a gag or envelope protein of an HIV isolate.
References Cited (Backward Citations)
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