Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
94 words
A method for treatment of solubilized collagen to inactivate prions and other infective agents, comprising: contacting solubilized collagen with sodium hydroxide in a liquid solution, whereby surface area of the solubilized collagen molecules is exposed, to expose prions and other infectious agents for treatment, wherein sodium hydroxide in said liquid solution ranges in concentration from about 0.1M to about 0.35M, for a time period sufficient to inactivate said prions and other infective agents, at a temperature of about 25° C. or less; wherein the collagen is capable of forming stable fibers after said inactivation.
Claim 2
Independent Claim
94 words
A method for treatment of solubilized collagen to inactivate prions and other infective agents, comprising: contacting solubilized collagen with sodium hydroxide in a liquid solution, whereby surface area of the solubilized collagen molecules is exposed, to expose prions and other infectious agents for treatment, wherein sodium hydroxide in said liquid solution ranges in concentration from about 0.1M to about 0.35M, for a time period sufficient to inactivate said prions and other infective agents, at a temperature of about 25° C. or less; wherein the collagen is capable of forming stable fibers after said inactivation.
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein said time period is about 80 minutes.
Claim 4
Dependent Claim
13 words
The method of claim 1, wherein said time period is about 100 minutes.
Claim 5
Dependent Claim
13 words
The method of claim 1, wherein said time period is about 150 minutes.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein the collagen is atelopeptide collagen.
Claim 7
Dependent Claim
12 words
The method of claim 1 wherein the temperature is about 20° C.
Claim 8
Dependent Claim
12 words
The method of claim 1 wherein the temperature is about 25° C.
Claim 9
Dependent Claim
18 words
The method of claim 1 wherein the method is effective to inactivate at least 5 logs of infectivity.
Claim 10
Dependent Claim
15 words
The method of claim 1 wherein the concentration is about 0.25M to 0.35M sodium hydroxide.
Claim 11
Dependent Claim
13 words
The method of claim 1 wherein the concentration is about 0.25M sodium hydroxide.
Claim 12
Dependent Claim
13 words
The method of claim 1, wherein said time period is about 90 minutes.
Claim 13
Dependent Claim
12 words
The method of claim 11 wherein the temperature is about 20° C.
Claim 14
Dependent Claim
12 words
The method of claim 11 wherein the temperature is about 25° C.
Claim 15
Dependent Claim
18 words
The method of claim 1, wherein the concentration of sodium hydroxide in said liquid solution is about 0.1M.
Claim 16
Dependent Claim
11 words
The method of claim 14 wherein the collagen is atelopeptide collagen.
Claim 17
Dependent Claim
18 words
The method of claim 14 wherein the method is effective to inactivate at least 5 logs of infectivity.
Claim 18
Independent Claim
91 words
A method of inactivating prions in a collagen material, comprising a) solubilizing collagen in a liquid solution, whereby surface area of the solubilized collagen molecules is exposed, to expose prions; and b) contacting the collagen in the liquid solution with sodium hydroxide, such that the concentration of sodium hydroxide in said liquid solution ranges from about 0.1M to about 0.35M, for a time period sufficient to inactivate said prions, at a temperature of about 25° C. or less, and wherein the collagen is capable of forming stable fibers after said inactivation.
Claim 19
Independent Claim
91 words
A method of inactivating prions in a collagen material, comprising a) solubilizing collagen in a liquid solution, whereby surface area of the solubilized collagen molecules is exposed, to expose prions; and b) contacting the collagen in the liquid solution with sodium hydroxide, such that the concentration of sodium hydroxide in said liquid solution ranges from about 0.1M to about 0.35M, for a time period sufficient to inactivate said prions, at a temperature of about 25° C. or less, and wherein the collagen is capable of forming stable fibers after said inactivation.
Claim 20
Dependent Claim
11 words
The method of claim 17 wherein the collagen is atelopeptide collagen.